Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3321

Experiment: LB_plus_SM_buffer with P22_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3320 and GFF3321 are separated by 754 nucleotidesGFF3321 and GFF3322 are separated by 41 nucleotidesGFF3322 and GFF3323 are separated by 61 nucleotides GFF3320 - Transcriptional regulator CsgD for 2nd curli operon, at 91,779 to 92,429 GFF3320 GFF3321 - Minor curlin subunit CsgB, nucleation component of curlin monomers, at 93,184 to 93,639 GFF3321 GFF3322 - Major curlin subunit precursor CsgA, at 93,681 to 94,136 GFF3322 GFF3323 - Putative curli production protein CsgC, at 94,198 to 94,524 GFF3323 Position (kb) 93 94Strain fitness (log2 ratio) -1 0 1 2at 92.392 kb on - strandat 92.392 kb on - strandat 92.409 kb on - strandat 93.508 kb on + strand, within GFF3321at 93.612 kb on + strandat 93.613 kb on - strandat 93.638 kb on - strandat 93.718 kb on + strandat 93.945 kb on - strand, within GFF3322at 93.945 kb on - strand, within GFF3322at 94.132 kb on - strandat 94.396 kb on - strand, within GFF3323at 94.464 kb on + strand, within GFF3323

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI
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92,392 - -0.7
92,392 - +0.1
92,409 - +0.1
93,508 + GFF3321 0.71 +0.9
93,612 + +0.6
93,613 - +1.9
93,638 - +0.8
93,718 + +0.6
93,945 - GFF3322 0.58 -0.2
93,945 - GFF3322 0.58 -0.1
94,132 - -0.5
94,396 - GFF3323 0.61 -0.9
94,464 + GFF3323 0.81 +0.1

Or see this region's nucleotide sequence