Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3177 and GFF3178 are separated by 123 nucleotides GFF3178 and GFF3215 are separated by 125 nucleotides GFF3215 and GFF3179 are separated by 79 nucleotides
GFF3177 - RNA polymerase sigma factor RpoD, at 181,536 to 183,383
GFF3177
GFF3178 - G:T/U mismatch-specific uracil/thymine DNA-glycosylase, at 183,507 to 184,013
GFF3178
GFF3215 - tRNA-Met-CAT, at 184,139 to 184,214
GFF3215
GFF3179 - iron-chelator utilization protein, at 184,294 to 185,061
GFF3179
Position (kb) 183
184
185 Strain fitness (log2 ratio) -2
-1
0
1
2 at 183.398 kb on - strand at 183.398 kb on - strand at 183.508 kb on + strand at 183.522 kb on - strand at 183.583 kb on - strand, within GFF3178 at 183.759 kb on + strand, within GFF3178 at 183.894 kb on - strand, within GFF3178 at 184.015 kb on - strand at 184.017 kb on + strand at 184.017 kb on + strand at 184.018 kb on - strand at 184.031 kb on - strand at 184.048 kb on + strand at 184.392 kb on + strand, within GFF3179 at 184.476 kb on - strand, within GFF3179 at 184.476 kb on - strand, within GFF3179 at 184.485 kb on + strand, within GFF3179 at 184.485 kb on + strand, within GFF3179 at 184.486 kb on - strand, within GFF3179 at 184.598 kb on - strand, within GFF3179 at 184.629 kb on - strand, within GFF3179 at 184.833 kb on + strand, within GFF3179 at 184.975 kb on - strand, within GFF3179 at 184.977 kb on + strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179 at 184.978 kb on - strand, within GFF3179
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 183,398 - +0.0 183,398 - -0.9 183,508 + +0.9 183,522 - +0.1 183,583 - GFF3178 0.15 -0.7 183,759 + GFF3178 0.50 -0.1 183,894 - GFF3178 0.76 -2.4 184,015 - -0.1 184,017 + -1.8 184,017 + -0.9 184,018 - -0.3 184,031 - +2.6 184,048 + +1.3 184,392 + GFF3179 0.13 -0.9 184,476 - GFF3179 0.24 -2.1 184,476 - GFF3179 0.24 +0.8 184,485 + GFF3179 0.25 +1.1 184,485 + GFF3179 0.25 +1.6 184,486 - GFF3179 0.25 +1.2 184,598 - GFF3179 0.40 +1.3 184,629 - GFF3179 0.44 +1.8 184,833 + GFF3179 0.70 -0.4 184,975 - GFF3179 0.89 -1.3 184,977 + GFF3179 0.89 +2.1 184,978 - GFF3179 0.89 -1.1 184,978 - GFF3179 0.89 +0.2 184,978 - GFF3179 0.89 -0.8
Or see this region's nucleotide sequence