Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2913 and GFF2914 are separated by 85 nucleotides
GFF2913 - Argininosuccinate synthase (EC 6.3.4.5), at 399 to 1,742
GFF2913
GFF2914 - putative cytoplasmic protein, at 1,828 to 2,892
GFF2914
Position (kb) 0
1
2 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 0.076 kb on - strand at 0.133 kb on + strand at 0.149 kb on + strand at 0.149 kb on + strand at 0.151 kb on + strand at 0.170 kb on - strand at 0.170 kb on - strand at 0.220 kb on + strand at 0.220 kb on + strand at 0.221 kb on - strand at 0.221 kb on - strand at 0.255 kb on - strand at 0.394 kb on - strand at 0.519 kb on + strand at 0.519 kb on + strand at 0.519 kb on + strand at 0.520 kb on - strand at 0.671 kb on + strand, within GFF2913 at 0.675 kb on - strand, within GFF2913 at 0.694 kb on - strand, within GFF2913 at 0.771 kb on - strand, within GFF2913 at 0.789 kb on - strand, within GFF2913 at 0.792 kb on + strand, within GFF2913 at 0.792 kb on + strand, within GFF2913 at 0.792 kb on + strand, within GFF2913 at 0.792 kb on + strand, within GFF2913 at 0.793 kb on - strand, within GFF2913 at 0.793 kb on - strand, within GFF2913 at 0.793 kb on - strand, within GFF2913 at 0.793 kb on - strand, within GFF2913 at 0.819 kb on + strand, within GFF2913 at 0.859 kb on - strand, within GFF2913 at 0.885 kb on - strand, within GFF2913 at 0.948 kb on - strand, within GFF2913 at 1.542 kb on + strand, within GFF2913 at 1.542 kb on + strand, within GFF2913 at 1.543 kb on - strand, within GFF2913 at 1.543 kb on - strand, within GFF2913 at 1.544 kb on + strand, within GFF2913 at 1.582 kb on + strand, within GFF2913 at 1.791 kb on + strand at 1.897 kb on + strand at 1.898 kb on - strand at 1.898 kb on - strand at 1.898 kb on - strand at 1.899 kb on + strand at 1.998 kb on + strand, within GFF2914 at 2.029 kb on - strand, within GFF2914 at 2.139 kb on - strand, within GFF2914 at 2.224 kb on - strand, within GFF2914 at 2.256 kb on + strand, within GFF2914 at 2.304 kb on + strand, within GFF2914 at 2.305 kb on - strand, within GFF2914 at 2.309 kb on - strand, within GFF2914 at 2.355 kb on + strand, within GFF2914 at 2.356 kb on - strand, within GFF2914 at 2.528 kb on + strand, within GFF2914 at 2.654 kb on - strand, within GFF2914 at 2.656 kb on + strand, within GFF2914
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 76 - +1.8 133 + +1.6 149 + +0.2 149 + -3.2 151 + +0.3 170 - -1.1 170 - -2.9 220 + +0.5 220 + +2.0 221 - -0.4 221 - +1.0 255 - +0.8 394 - -2.3 519 + -0.7 519 + +2.5 519 + -0.5 520 - -2.4 671 + GFF2913 0.20 -0.1 675 - GFF2913 0.21 -0.6 694 - GFF2913 0.22 +0.5 771 - GFF2913 0.28 -0.6 789 - GFF2913 0.29 -1.1 792 + GFF2913 0.29 -0.6 792 + GFF2913 0.29 -2.8 792 + GFF2913 0.29 +0.2 792 + GFF2913 0.29 +0.3 793 - GFF2913 0.29 -0.2 793 - GFF2913 0.29 -1.2 793 - GFF2913 0.29 +0.3 793 - GFF2913 0.29 +2.2 819 + GFF2913 0.31 +3.0 859 - GFF2913 0.34 +1.3 885 - GFF2913 0.36 -1.4 948 - GFF2913 0.41 -0.3 1,542 + GFF2913 0.85 +0.1 1,542 + GFF2913 0.85 +0.6 1,543 - GFF2913 0.85 -2.4 1,543 - GFF2913 0.85 -1.4 1,544 + GFF2913 0.85 -0.6 1,582 + GFF2913 0.88 -1.3 1,791 + +1.1 1,897 + -0.7 1,898 - +2.1 1,898 - +0.2 1,898 - +1.1 1,899 + +0.1 1,998 + GFF2914 0.16 +0.0 2,029 - GFF2914 0.19 -1.1 2,139 - GFF2914 0.29 -1.3 2,224 - GFF2914 0.37 -0.1 2,256 + GFF2914 0.40 +0.6 2,304 + GFF2914 0.45 +0.8 2,305 - GFF2914 0.45 +0.1 2,309 - GFF2914 0.45 +0.2 2,355 + GFF2914 0.49 +0.2 2,356 - GFF2914 0.50 +1.0 2,528 + GFF2914 0.66 +0.7 2,654 - GFF2914 0.78 -0.4 2,656 + GFF2914 0.78 +1.9
Or see this region's nucleotide sequence