Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF282 and GFF283 overlap by 2 nucleotides GFF283 and GFF284 are separated by 257 nucleotides GFF284 and GFF285 are separated by 109 nucleotides GFF285 and GFF286 are separated by 189 nucleotides
GFF282 - Outer membrane lipoprotein YidQ, at 2,107 to 2,367
GFF282
GFF283 - hypothetical protein, at 2,366 to 2,485
GFF283
GFF284 - 16 kDa heat shock protein A, at 2,743 to 3,162
GFF284
GFF285 - 16 kDa heat shock protein B, at 3,272 to 3,700
GFF285
GFF286 - Mediator of hyperadherence YidE, at 3,890 to 5,551
GFF286
Position (kb)
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2.336 kb on + strand, within GFF282 at 2.336 kb on + strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.341 kb on - strand, within GFF282 at 2.390 kb on - strand, within GFF283 at 2.390 kb on - strand, within GFF283 at 2.410 kb on + strand, within GFF283 at 2.481 kb on + strand at 2.484 kb on - strand at 2.613 kb on + strand at 2.624 kb on + strand at 2.628 kb on + strand at 2.642 kb on + strand at 2.644 kb on + strand at 2.651 kb on + strand at 2.652 kb on - strand at 2.742 kb on + strand at 2.823 kb on + strand, within GFF284 at 2.887 kb on + strand, within GFF284 at 2.997 kb on + strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 2.998 kb on - strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.001 kb on + strand, within GFF284 at 3.002 kb on - strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.039 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.100 kb on + strand, within GFF284 at 3.101 kb on - strand, within GFF284 at 3.102 kb on + strand, within GFF284 at 3.255 kb on + strand at 3.256 kb on - strand at 3.271 kb on + strand at 3.278 kb on - strand at 3.281 kb on + strand at 3.282 kb on - strand at 3.376 kb on + strand, within GFF285 at 3.413 kb on - strand, within GFF285 at 3.620 kb on - strand, within GFF285 at 3.690 kb on + strand at 3.695 kb on - strand at 3.698 kb on + strand at 3.699 kb on - strand at 3.699 kb on - strand at 3.824 kb on + strand at 3.824 kb on + strand at 3.825 kb on - strand at 3.825 kb on - strand at 3.826 kb on + strand at 3.826 kb on + strand at 3.827 kb on - strand at 3.827 kb on - strand at 3.827 kb on - strand at 3.828 kb on + strand at 3.828 kb on + strand at 3.828 kb on + strand at 3.829 kb on - strand at 3.961 kb on + strand at 4.012 kb on + strand at 4.195 kb on + strand, within GFF286 at 4.368 kb on - strand, within GFF286 at 4.368 kb on - strand, within GFF286 at 4.380 kb on - strand, within GFF286 at 4.497 kb on + strand, within GFF286 at 4.540 kb on + strand, within GFF286 at 4.540 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286 at 4.543 kb on + strand, within GFF286
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 2,336 + GFF282 0.88 +1.0 2,336 + GFF282 0.88 +0.6 2,341 - GFF282 0.90 +1.8 2,341 - GFF282 0.90 +0.1 2,341 - GFF282 0.90 -0.6 2,390 - GFF283 0.20 -0.7 2,390 - GFF283 0.20 +0.1 2,410 + GFF283 0.37 -0.6 2,481 + +0.2 2,484 - -1.4 2,613 + +0.5 2,624 + -1.8 2,628 + +0.0 2,642 + +0.5 2,644 + -0.6 2,651 + +1.8 2,652 - +2.6 2,742 + -1.2 2,823 + GFF284 0.19 +0.2 2,887 + GFF284 0.34 -0.8 2,997 + GFF284 0.60 -1.2 2,998 - GFF284 0.61 -0.1 2,998 - GFF284 0.61 -0.9 3,001 + GFF284 0.61 +0.2 3,001 + GFF284 0.61 +1.4 3,002 - GFF284 0.62 -0.1 3,039 + GFF284 0.70 -0.5 3,039 + GFF284 0.70 -0.8 3,100 + GFF284 0.85 +0.8 3,100 + GFF284 0.85 -0.8 3,101 - GFF284 0.85 +0.9 3,102 + GFF284 0.85 -0.4 3,255 + -1.1 3,256 - -1.6 3,271 + -0.8 3,278 - -0.1 3,281 + -0.0 3,282 - -0.1 3,376 + GFF285 0.24 -3.4 3,413 - GFF285 0.33 -0.7 3,620 - GFF285 0.81 -0.7 3,690 + +0.3 3,695 - -0.3 3,698 + -0.9 3,699 - +0.7 3,699 - +0.8 3,824 + -0.0 3,824 + -1.6 3,825 - -1.5 3,825 - -0.1 3,826 + -0.2 3,826 + -0.1 3,827 - -0.3 3,827 - +0.6 3,827 - -0.8 3,828 + -1.1 3,828 + +0.2 3,828 + -0.4 3,829 - -0.8 3,961 + -1.0 4,012 + -1.6 4,195 + GFF286 0.18 -0.9 4,368 - GFF286 0.29 -0.5 4,368 - GFF286 0.29 -0.4 4,380 - GFF286 0.29 -0.6 4,497 + GFF286 0.37 -1.4 4,540 + GFF286 0.39 +0.2 4,540 + GFF286 0.39 -3.7 4,543 + GFF286 0.39 -1.7 4,543 + GFF286 0.39 +0.1 4,543 + GFF286 0.39 -0.7 4,543 + GFF286 0.39 +0.3
Or see this region's nucleotide sequence