Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2835 and GFF2836 overlap by 4 nucleotides GFF2836 and GFF2837 are separated by 65 nucleotides GFF2837 and GFF2838 are separated by 219 nucleotides
GFF2835 - Putative membrane protein, at 357,416 to 357,982
GFF2835
GFF2836 - Ribosomal RNA large subunit methyltransferase A (EC 2.1.1.51), at 357,979 to 358,788
GFF2836
GFF2837 - Cell division protein FtsI [Peptidoglycan synthetase] (EC 2.4.1.129), at 358,854 to 360,599
GFF2837
GFF2838 - Cold shock protein CspC, at 360,819 to 361,028
GFF2838
Position (kb) 358
359
360
361 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 357.864 kb on + strand, within GFF2835 at 358.137 kb on - strand, within GFF2836 at 358.229 kb on - strand, within GFF2836 at 358.311 kb on + strand, within GFF2836 at 358.434 kb on + strand, within GFF2836 at 358.497 kb on - strand, within GFF2836 at 358.564 kb on - strand, within GFF2836 at 358.663 kb on - strand, within GFF2836 at 358.729 kb on + strand at 358.730 kb on - strand at 358.730 kb on - strand at 358.731 kb on + strand at 358.732 kb on - strand at 358.740 kb on + strand at 358.780 kb on - strand at 358.919 kb on + strand at 359.025 kb on - strand at 359.079 kb on - strand, within GFF2837 at 359.081 kb on - strand, within GFF2837 at 359.090 kb on - strand, within GFF2837 at 359.090 kb on - strand, within GFF2837 at 359.090 kb on - strand, within GFF2837 at 359.150 kb on + strand, within GFF2837 at 359.213 kb on + strand, within GFF2837 at 359.214 kb on - strand, within GFF2837 at 359.481 kb on - strand, within GFF2837 at 359.482 kb on + strand, within GFF2837 at 359.493 kb on + strand, within GFF2837 at 360.047 kb on + strand, within GFF2837 at 360.304 kb on + strand, within GFF2837 at 360.325 kb on - strand, within GFF2837 at 360.446 kb on + strand at 360.446 kb on + strand at 360.446 kb on + strand at 360.556 kb on - strand at 360.832 kb on - strand at 360.937 kb on - strand, within GFF2838 at 361.017 kb on - strand at 361.107 kb on - strand at 361.110 kb on - strand at 361.197 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 357,864 + GFF2835 0.79 +1.2 358,137 - GFF2836 0.20 +1.0 358,229 - GFF2836 0.31 +1.2 358,311 + GFF2836 0.41 +3.2 358,434 + GFF2836 0.56 +0.9 358,497 - GFF2836 0.64 +0.9 358,564 - GFF2836 0.72 -0.1 358,663 - GFF2836 0.84 +0.4 358,729 + -0.1 358,730 - -0.1 358,730 - -0.8 358,731 + +1.2 358,732 - -0.3 358,740 + +1.0 358,780 - -0.0 358,919 + +1.1 359,025 - +0.1 359,079 - GFF2837 0.13 +2.1 359,081 - GFF2837 0.13 +1.4 359,090 - GFF2837 0.14 +0.1 359,090 - GFF2837 0.14 +1.1 359,090 - GFF2837 0.14 +0.4 359,150 + GFF2837 0.17 +0.7 359,213 + GFF2837 0.21 +2.0 359,214 - GFF2837 0.21 +2.4 359,481 - GFF2837 0.36 -1.0 359,482 + GFF2837 0.36 +0.7 359,493 + GFF2837 0.37 +0.9 360,047 + GFF2837 0.68 +2.0 360,304 + GFF2837 0.83 +1.0 360,325 - GFF2837 0.84 +0.9 360,446 + +1.1 360,446 + +1.2 360,446 + +0.1 360,556 - -1.8 360,832 - +0.3 360,937 - GFF2838 0.56 +0.6 361,017 - +1.7 361,107 - +1.3 361,110 - -1.5 361,197 - +1.1
Or see this region's nucleotide sequence