Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2819 and GFF2820 are separated by 84 nucleotides GFF2820 and GFF2821 are separated by 204 nucleotides GFF2821 and GFF2822 are separated by 71 nucleotides
GFF2819 - Ribonuclease D (EC 3.1.26.3), at 339,291 to 340,406
GFF2819
GFF2820 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 340,491 to 342,176
GFF2820
GFF2821 - Starvation lipoprotein Slp paralog, at 342,381 to 342,962
GFF2821
GFF2822 - TsaB protein, required for threonylcarbamoyladenosine (t(6)A) formation in tRNA, at 343,034 to 343,729
GFF2822
Position (kb)
340
341
342
343 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 339.669 kb on - strand, within GFF2819 at 339.947 kb on - strand, within GFF2819 at 340.045 kb on + strand, within GFF2819 at 340.046 kb on - strand, within GFF2819 at 340.054 kb on - strand, within GFF2819 at 340.156 kb on - strand, within GFF2819 at 340.310 kb on - strand at 340.604 kb on + strand at 340.604 kb on + strand at 340.605 kb on - strand at 341.108 kb on - strand, within GFF2820 at 341.256 kb on - strand, within GFF2820 at 341.486 kb on + strand, within GFF2820 at 341.537 kb on + strand, within GFF2820 at 341.539 kb on + strand, within GFF2820 at 341.694 kb on - strand, within GFF2820 at 341.736 kb on - strand, within GFF2820 at 341.764 kb on - strand, within GFF2820 at 341.789 kb on + strand, within GFF2820 at 341.905 kb on - strand, within GFF2820 at 342.033 kb on + strand at 342.424 kb on + strand at 342.424 kb on + strand at 342.561 kb on - strand, within GFF2821 at 342.563 kb on - strand, within GFF2821 at 342.621 kb on + strand, within GFF2821 at 342.622 kb on - strand, within GFF2821 at 342.622 kb on - strand, within GFF2821 at 342.812 kb on - strand, within GFF2821 at 342.939 kb on + strand at 342.940 kb on - strand at 343.026 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 339,669 - GFF2819 0.34 +2.1 339,947 - GFF2819 0.59 +1.6 340,045 + GFF2819 0.68 +2.7 340,046 - GFF2819 0.68 +1.2 340,054 - GFF2819 0.68 +1.6 340,156 - GFF2819 0.78 +1.7 340,310 - +1.0 340,604 + +1.0 340,604 + +0.8 340,605 - +2.6 341,108 - GFF2820 0.37 +2.3 341,256 - GFF2820 0.45 +1.2 341,486 + GFF2820 0.59 +1.0 341,537 + GFF2820 0.62 +0.7 341,539 + GFF2820 0.62 +3.0 341,694 - GFF2820 0.71 +1.9 341,736 - GFF2820 0.74 +1.2 341,764 - GFF2820 0.76 +0.9 341,789 + GFF2820 0.77 +1.3 341,905 - GFF2820 0.84 +0.6 342,033 + -0.5 342,424 + +1.2 342,424 + +1.3 342,561 - GFF2821 0.31 -1.3 342,563 - GFF2821 0.31 +0.7 342,621 + GFF2821 0.41 -1.9 342,622 - GFF2821 0.41 -0.7 342,622 - GFF2821 0.41 +0.9 342,812 - GFF2821 0.74 +0.2 342,939 + +1.1 342,940 - +1.7 343,026 - -0.9
Or see this region's nucleotide sequence