Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2733 and GFF2734 are separated by 56 nucleotides GFF2734 and GFF2735 are separated by 169 nucleotides GFF2735 and GFF2736 are separated by 59 nucleotides
GFF2733 - Membrane protein YciC, linked to IspA, at 254,917 to 255,660
GFF2733
GFF2734 - Intracellular septation protein IspA, at 255,717 to 256,256
GFF2734
GFF2735 - Acyl-CoA thioesterase YciA, involved in membrane biogenesis, at 256,426 to 256,818
GFF2735
GFF2736 - Ferric siderophore transport system, periplasmic binding protein TonB, at 256,878 to 257,606
GFF2736
Position (kb) 256
257 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 255.625 kb on + strand at 255.640 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.642 kb on + strand at 255.643 kb on - strand at 255.658 kb on + strand at 255.783 kb on + strand, within GFF2734 at 255.876 kb on - strand, within GFF2734 at 255.988 kb on + strand, within GFF2734 at 256.254 kb on + strand at 256.271 kb on - strand at 256.434 kb on + strand at 256.458 kb on - strand at 256.461 kb on + strand at 256.581 kb on - strand, within GFF2735 at 256.638 kb on - strand, within GFF2735 at 256.678 kb on - strand, within GFF2735 at 256.686 kb on - strand, within GFF2735 at 256.853 kb on + strand at 257.088 kb on + strand, within GFF2736 at 257.593 kb on - strand at 257.700 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 255,625 + -0.0 255,640 + -0.2 255,642 + -0.3 255,642 + -0.1 255,642 + -0.5 255,642 + -0.1 255,642 + +0.2 255,643 - +1.3 255,658 + +0.5 255,783 + GFF2734 0.12 -1.8 255,876 - GFF2734 0.29 +2.7 255,988 + GFF2734 0.50 +1.0 256,254 + -3.8 256,271 - +0.3 256,434 + +0.2 256,458 - -1.5 256,461 + +0.2 256,581 - GFF2735 0.39 +2.0 256,638 - GFF2735 0.54 -0.6 256,678 - GFF2735 0.64 +2.4 256,686 - GFF2735 0.66 +0.5 256,853 + +1.2 257,088 + GFF2736 0.29 +0.3 257,593 - +0.1 257,700 - +0.7
Or see this region's nucleotide sequence