Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2674 and GFF2675 are separated by 96 nucleotides GFF2675 and GFF2676 are separated by 189 nucleotides GFF2676 and GFF2677 overlap by 26 nucleotides
GFF2674 - FIG00553873: hypothetical protein, at 196,589 to 197,518
GFF2674
GFF2675 - Periplasmic Murein Peptide-Binding Protein MppA, at 197,615 to 199,228
GFF2675
GFF2676 - Gamma-D-Glutamyl-meso-Diaminopimelate Amidase, at 199,418 to 200,146
GFF2676
GFF2677 - L-alanine-DL-glutamate epimerase, at 200,121 to 201,086
GFF2677
Position (kb) 197
198
199
200 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 196.874 kb on - strand, within GFF2674 at 197.086 kb on - strand, within GFF2674 at 197.090 kb on - strand, within GFF2674 at 197.329 kb on + strand, within GFF2674 at 197.341 kb on - strand, within GFF2674 at 197.432 kb on - strand at 197.632 kb on - strand at 197.911 kb on - strand, within GFF2675 at 198.286 kb on + strand, within GFF2675 at 198.941 kb on - strand, within GFF2675 at 198.941 kb on - strand, within GFF2675 at 198.953 kb on - strand, within GFF2675 at 199.085 kb on + strand at 199.377 kb on + strand at 199.476 kb on - strand at 199.504 kb on - strand, within GFF2676 at 199.614 kb on + strand, within GFF2676 at 199.614 kb on + strand, within GFF2676 at 199.645 kb on - strand, within GFF2676 at 199.681 kb on - strand, within GFF2676 at 199.708 kb on - strand, within GFF2676 at 199.848 kb on + strand, within GFF2676 at 199.978 kb on - strand, within GFF2676 at 199.978 kb on - strand, within GFF2676
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 196,874 - GFF2674 0.31 -0.4 197,086 - GFF2674 0.53 +0.1 197,090 - GFF2674 0.54 -0.3 197,329 + GFF2674 0.80 +1.5 197,341 - GFF2674 0.81 -2.0 197,432 - +3.0 197,632 - -1.3 197,911 - GFF2675 0.18 -3.0 198,286 + GFF2675 0.42 +0.4 198,941 - GFF2675 0.82 -3.4 198,941 - GFF2675 0.82 -3.1 198,953 - GFF2675 0.83 -0.3 199,085 + -2.6 199,377 + -0.5 199,476 - -3.0 199,504 - GFF2676 0.12 +0.6 199,614 + GFF2676 0.27 -0.3 199,614 + GFF2676 0.27 +0.0 199,645 - GFF2676 0.31 -0.8 199,681 - GFF2676 0.36 -1.8 199,708 - GFF2676 0.40 +0.3 199,848 + GFF2676 0.59 -2.1 199,978 - GFF2676 0.77 -0.1 199,978 - GFF2676 0.77 -0.5
Or see this region's nucleotide sequence