Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2494 and GFF2495 are separated by 66 nucleotides GFF2495 and GFF2496 are separated by 180 nucleotides GFF2496 and GFF2497 are separated by 129 nucleotides
GFF2494 - D-mannonate oxidoreductase (EC 1.1.1.57), at 11,686 to 13,152
GFF2494
GFF2495 - FIG00638357: hypothetical protein, at 13,219 to 13,422
GFF2495
GFF2496 - Transcriptional regulator, GntR family, at 13,603 to 14,289
GFF2496
GFF2497 - 3-hydroxypropionate dehydrogenase (EC 1.1.1.298), at 14,419 to 15,165
GFF2497
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 12.615 kb on + strand, within GFF2494 at 12.616 kb on - strand, within GFF2494 at 12.616 kb on - strand, within GFF2494 at 12.618 kb on + strand, within GFF2494 at 12.708 kb on - strand, within GFF2494 at 12.713 kb on + strand, within GFF2494 at 12.721 kb on + strand, within GFF2494 at 12.771 kb on + strand, within GFF2494 at 13.413 kb on - strand at 13.486 kb on - strand at 13.487 kb on + strand at 13.731 kb on + strand, within GFF2496 at 13.785 kb on + strand, within GFF2496 at 13.785 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.926 kb on + strand, within GFF2496 at 13.927 kb on - strand, within GFF2496 at 13.958 kb on + strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.070 kb on - strand, within GFF2496 at 14.121 kb on + strand, within GFF2496 at 14.347 kb on + strand at 14.374 kb on + strand at 14.413 kb on - strand at 14.420 kb on + strand at 14.557 kb on - strand, within GFF2497 at 14.727 kb on - strand, within GFF2497 at 15.090 kb on + strand, within GFF2497 at 15.152 kb on - strand at 15.195 kb on + strand at 15.195 kb on + strand at 15.199 kb on - strand at 15.203 kb on + strand at 15.228 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 12,615 + GFF2494 0.63 -0.3 12,616 - GFF2494 0.63 -0.1 12,616 - GFF2494 0.63 +0.7 12,618 + GFF2494 0.64 +0.6 12,708 - GFF2494 0.70 +2.3 12,713 + GFF2494 0.70 +0.9 12,721 + GFF2494 0.71 -0.1 12,771 + GFF2494 0.74 +0.0 13,413 - -2.7 13,486 - -1.2 13,487 + +0.5 13,731 + GFF2496 0.19 +1.3 13,785 + GFF2496 0.26 +0.8 13,785 + GFF2496 0.26 +3.4 13,926 + GFF2496 0.47 +2.0 13,926 + GFF2496 0.47 +0.1 13,926 + GFF2496 0.47 -1.8 13,926 + GFF2496 0.47 +1.0 13,927 - GFF2496 0.47 +0.7 13,958 + GFF2496 0.52 -0.1 14,070 - GFF2496 0.68 -1.6 14,070 - GFF2496 0.68 +1.3 14,070 - GFF2496 0.68 +0.5 14,121 + GFF2496 0.75 -0.3 14,347 + +1.2 14,374 + +0.6 14,413 - -0.4 14,420 + +0.6 14,557 - GFF2497 0.18 -0.0 14,727 - GFF2497 0.41 +0.3 15,090 + GFF2497 0.90 -0.2 15,152 - -2.0 15,195 + +0.1 15,195 + -0.4 15,199 - -0.4 15,203 + -0.5 15,228 - +1.2
Or see this region's nucleotide sequence