Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2431 and GFF2432 are separated by 12 nucleotides GFF2432 and GFF2433 are separated by 195 nucleotides GFF2433 and GFF2434 are separated by 28 nucleotides GFF2434 and GFF2435 are separated by 27 nucleotides
GFF2431 - Chaperone protein HscA, at 46,681 to 48,531
GFF2431
GFF2432 - Chaperone protein HscB, at 48,544 to 49,059
GFF2432
GFF2433 - Iron binding protein IscA for iron-sulfur cluster assembly, at 49,255 to 49,578
GFF2433
GFF2434 - Iron-sulfur cluster assembly scaffold protein IscU, at 49,607 to 49,993
GFF2434
GFF2435 - Cysteine desulfurase (EC 2.8.1.7), IscS subfamily, at 50,021 to 51,235
GFF2435
Position (kb) 49
50 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 48.448 kb on - strand at 48.535 kb on - strand at 48.808 kb on - strand, within GFF2432 at 48.808 kb on - strand, within GFF2432 at 49.121 kb on + strand at 49.122 kb on - strand at 49.237 kb on + strand at 49.257 kb on - strand at 49.460 kb on + strand, within GFF2433 at 49.460 kb on + strand, within GFF2433 at 49.460 kb on + strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.461 kb on - strand, within GFF2433 at 49.579 kb on - strand at 49.633 kb on - strand at 49.919 kb on - strand, within GFF2434 at 50.068 kb on - strand at 50.113 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 48,448 - +0.3 48,535 - -1.5 48,808 - GFF2432 0.51 -0.7 48,808 - GFF2432 0.51 -1.5 49,121 + -2.5 49,122 - +0.9 49,237 + -0.2 49,257 - -2.9 49,460 + GFF2433 0.63 -1.1 49,460 + GFF2433 0.63 +1.9 49,460 + GFF2433 0.63 -0.3 49,461 - GFF2433 0.64 -0.7 49,461 - GFF2433 0.64 -1.1 49,461 - GFF2433 0.64 +2.5 49,461 - GFF2433 0.64 -0.7 49,461 - GFF2433 0.64 -3.1 49,579 - +0.7 49,633 - -1.9 49,919 - GFF2434 0.81 +0.1 50,068 - -2.6 50,113 + +1.3
Or see this region's nucleotide sequence