Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2420 and GFF2421 are separated by 0 nucleotides GFF2421 and GFF2422 are separated by 208 nucleotides
GFF2420 - Penicillin-insensitive transglycosylase (EC 2.4.2.-) & transpeptidase PBP-1C, at 33,931 to 36,246
GFF2420
GFF2421 - Alpha-2-macroglobulin, at 36,247 to 41,181
GFF2421
GFF2422 - Thiosulfate sulfurtransferase, rhodanese (EC 2.8.1.1), at 41,390 to 42,232
GFF2422
Position (kb) 36
37
38
39
40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 35.281 kb on - strand, within GFF2420 at 35.317 kb on - strand, within GFF2420 at 35.317 kb on - strand, within GFF2420 at 35.587 kb on - strand, within GFF2420 at 35.709 kb on - strand, within GFF2420 at 35.738 kb on - strand, within GFF2420 at 35.886 kb on + strand, within GFF2420 at 36.229 kb on - strand at 36.248 kb on + strand at 36.249 kb on - strand at 36.305 kb on + strand at 36.306 kb on - strand at 36.339 kb on - strand at 36.440 kb on + strand at 36.441 kb on - strand at 37.045 kb on - strand, within GFF2421 at 37.204 kb on - strand, within GFF2421 at 37.294 kb on - strand, within GFF2421 at 37.294 kb on - strand, within GFF2421 at 37.305 kb on + strand, within GFF2421 at 37.398 kb on + strand, within GFF2421 at 37.399 kb on - strand, within GFF2421 at 37.423 kb on - strand, within GFF2421 at 37.607 kb on + strand, within GFF2421 at 37.659 kb on - strand, within GFF2421 at 37.659 kb on - strand, within GFF2421 at 37.768 kb on + strand, within GFF2421 at 37.857 kb on + strand, within GFF2421 at 37.857 kb on + strand, within GFF2421 at 37.957 kb on + strand, within GFF2421 at 37.958 kb on - strand, within GFF2421 at 38.043 kb on + strand, within GFF2421 at 38.252 kb on - strand, within GFF2421 at 38.420 kb on + strand, within GFF2421 at 38.424 kb on + strand, within GFF2421 at 38.497 kb on + strand, within GFF2421 at 38.662 kb on + strand, within GFF2421 at 38.662 kb on + strand, within GFF2421 at 38.662 kb on + strand, within GFF2421 at 38.663 kb on - strand, within GFF2421 at 38.663 kb on - strand, within GFF2421 at 38.727 kb on + strand, within GFF2421 at 38.727 kb on + strand, within GFF2421 at 38.761 kb on - strand, within GFF2421 at 38.907 kb on - strand, within GFF2421 at 38.983 kb on - strand, within GFF2421 at 39.177 kb on - strand, within GFF2421 at 39.448 kb on + strand, within GFF2421 at 39.448 kb on + strand, within GFF2421 at 39.759 kb on + strand, within GFF2421 at 40.228 kb on - strand, within GFF2421 at 40.293 kb on + strand, within GFF2421 at 40.363 kb on - strand, within GFF2421 at 40.365 kb on + strand, within GFF2421 at 40.367 kb on + strand, within GFF2421 at 40.368 kb on - strand, within GFF2421 at 40.368 kb on - strand, within GFF2421 at 40.368 kb on - strand, within GFF2421 at 40.373 kb on + strand, within GFF2421 at 40.374 kb on - strand, within GFF2421 at 40.403 kb on + strand, within GFF2421 at 40.403 kb on + strand, within GFF2421 at 40.404 kb on - strand, within GFF2421 at 40.432 kb on - strand, within GFF2421 at 40.499 kb on + strand, within GFF2421 at 40.554 kb on + strand, within GFF2421 at 40.728 kb on + strand at 40.829 kb on - strand at 40.997 kb on - strand at 40.999 kb on + strand at 41.041 kb on + strand at 41.253 kb on + strand at 41.326 kb on + strand at 41.326 kb on + strand at 41.434 kb on + strand at 41.435 kb on - strand at 41.435 kb on - strand at 41.591 kb on - strand, within GFF2422 at 41.666 kb on + strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.667 kb on - strand, within GFF2422 at 41.989 kb on - strand, within GFF2422
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 35,281 - GFF2420 0.58 +0.7 35,317 - GFF2420 0.60 -2.8 35,317 - GFF2420 0.60 -0.3 35,587 - GFF2420 0.72 +0.3 35,709 - GFF2420 0.77 -0.3 35,738 - GFF2420 0.78 +0.8 35,886 + GFF2420 0.84 -3.2 36,229 - +0.1 36,248 + -0.7 36,249 - -0.1 36,305 + -0.1 36,306 - -2.9 36,339 - -1.3 36,440 + +0.2 36,441 - +0.2 37,045 - GFF2421 0.16 -1.1 37,204 - GFF2421 0.19 -3.3 37,294 - GFF2421 0.21 +0.3 37,294 - GFF2421 0.21 -1.4 37,305 + GFF2421 0.21 -0.9 37,398 + GFF2421 0.23 -2.1 37,399 - GFF2421 0.23 +2.6 37,423 - GFF2421 0.24 +0.4 37,607 + GFF2421 0.28 +1.3 37,659 - GFF2421 0.29 -1.8 37,659 - GFF2421 0.29 -0.1 37,768 + GFF2421 0.31 +0.5 37,857 + GFF2421 0.33 +2.0 37,857 + GFF2421 0.33 -0.7 37,957 + GFF2421 0.35 -1.9 37,958 - GFF2421 0.35 +0.2 38,043 + GFF2421 0.36 -2.5 38,252 - GFF2421 0.41 +0.9 38,420 + GFF2421 0.44 +1.9 38,424 + GFF2421 0.44 -0.4 38,497 + GFF2421 0.46 -0.3 38,662 + GFF2421 0.49 +0.0 38,662 + GFF2421 0.49 +0.8 38,662 + GFF2421 0.49 +2.0 38,663 - GFF2421 0.49 -0.7 38,663 - GFF2421 0.49 +0.4 38,727 + GFF2421 0.50 -1.3 38,727 + GFF2421 0.50 -1.1 38,761 - GFF2421 0.51 -1.5 38,907 - GFF2421 0.54 +1.3 38,983 - GFF2421 0.55 -2.5 39,177 - GFF2421 0.59 -1.9 39,448 + GFF2421 0.65 -0.7 39,448 + GFF2421 0.65 -2.4 39,759 + GFF2421 0.71 +0.8 40,228 - GFF2421 0.81 +0.2 40,293 + GFF2421 0.82 -0.2 40,363 - GFF2421 0.83 -3.1 40,365 + GFF2421 0.83 -1.9 40,367 + GFF2421 0.83 -0.3 40,368 - GFF2421 0.84 -1.7 40,368 - GFF2421 0.84 +0.3 40,368 - GFF2421 0.84 +0.1 40,373 + GFF2421 0.84 -1.5 40,374 - GFF2421 0.84 -0.6 40,403 + GFF2421 0.84 -1.1 40,403 + GFF2421 0.84 +2.2 40,404 - GFF2421 0.84 +0.0 40,432 - GFF2421 0.85 +0.5 40,499 + GFF2421 0.86 -1.1 40,554 + GFF2421 0.87 -0.5 40,728 + +0.9 40,829 - -2.2 40,997 - +0.8 40,999 + -0.5 41,041 + +0.4 41,253 + +0.2 41,326 + -2.3 41,326 + -0.2 41,434 + -3.1 41,435 - -3.0 41,435 - -3.0 41,591 - GFF2422 0.24 +0.8 41,666 + GFF2422 0.33 -0.4 41,667 - GFF2422 0.33 -0.9 41,667 - GFF2422 0.33 -0.3 41,667 - GFF2422 0.33 -2.0 41,989 - GFF2422 0.71 +4.0
Or see this region's nucleotide sequence