Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2344 and GFF2345 are separated by 10 nucleotides GFF2345 and GFF2346 are separated by 117 nucleotides
GFF2344 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 138,437 to 139,441
GFF2344
GFF2345 - Acetylglutamate kinase (EC 2.7.2.8), at 139,452 to 140,225
GFF2345
GFF2346 - Argininosuccinate lyase (EC 4.3.2.1), at 140,343 to 141,719
GFF2346
Position (kb)
139
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 138.489 kb on + strand at 138.495 kb on - strand at 138.514 kb on + strand at 138.514 kb on + strand at 138.514 kb on + strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.515 kb on - strand at 138.579 kb on + strand, within GFF2344 at 138.634 kb on - strand, within GFF2344 at 138.777 kb on - strand, within GFF2344 at 138.777 kb on - strand, within GFF2344 at 138.834 kb on - strand, within GFF2344 at 138.972 kb on + strand, within GFF2344 at 139.055 kb on + strand, within GFF2344 at 139.165 kb on + strand, within GFF2344 at 139.165 kb on + strand, within GFF2344 at 139.188 kb on + strand, within GFF2344 at 139.227 kb on - strand, within GFF2344 at 139.254 kb on - strand, within GFF2344 at 139.400 kb on - strand at 139.435 kb on + strand at 139.436 kb on - strand at 139.466 kb on + strand at 139.483 kb on + strand at 139.486 kb on + strand at 139.486 kb on + strand at 139.537 kb on - strand, within GFF2345 at 139.700 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.075 kb on + strand, within GFF2345 at 140.079 kb on - strand, within GFF2345 at 140.224 kb on - strand at 140.224 kb on - strand at 140.228 kb on - strand at 140.228 kb on - strand at 140.266 kb on - strand at 140.266 kb on - strand at 140.339 kb on + strand at 140.340 kb on - strand at 140.366 kb on + strand at 140.442 kb on - strand at 140.591 kb on + strand, within GFF2346 at 140.597 kb on + strand, within GFF2346 at 140.802 kb on + strand, within GFF2346 at 140.804 kb on + strand, within GFF2346 at 140.804 kb on + strand, within GFF2346 at 140.805 kb on - strand, within GFF2346 at 140.856 kb on + strand, within GFF2346 at 140.904 kb on - strand, within GFF2346 at 140.996 kb on + strand, within GFF2346 at 140.997 kb on - strand, within GFF2346 at 141.143 kb on - strand, within GFF2346
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 138,489 + -0.2 138,495 - -1.8 138,514 + -0.1 138,514 + +1.5 138,514 + +1.0 138,515 - -0.1 138,515 - -2.4 138,515 - -1.9 138,515 - -0.9 138,579 + GFF2344 0.14 -2.2 138,634 - GFF2344 0.20 +0.4 138,777 - GFF2344 0.34 +0.1 138,777 - GFF2344 0.34 -1.8 138,834 - GFF2344 0.40 -0.9 138,972 + GFF2344 0.53 +0.9 139,055 + GFF2344 0.61 +0.0 139,165 + GFF2344 0.72 -0.4 139,165 + GFF2344 0.72 +0.3 139,188 + GFF2344 0.75 -0.0 139,227 - GFF2344 0.79 +0.2 139,254 - GFF2344 0.81 -0.6 139,400 - +0.4 139,435 + +1.2 139,436 - +0.5 139,466 + +0.0 139,483 + +0.3 139,486 + -1.7 139,486 + -1.5 139,537 - GFF2345 0.11 -1.9 139,700 + GFF2345 0.32 +0.0 140,075 + GFF2345 0.80 -0.7 140,075 + GFF2345 0.80 -1.9 140,075 + GFF2345 0.80 +0.1 140,079 - GFF2345 0.81 -0.5 140,224 - -1.3 140,224 - -0.2 140,228 - -1.1 140,228 - -1.1 140,266 - -0.5 140,266 - -0.7 140,339 + -1.3 140,340 - -1.2 140,366 + +2.2 140,442 - -1.9 140,591 + GFF2346 0.18 -1.5 140,597 + GFF2346 0.18 -0.8 140,802 + GFF2346 0.33 -0.9 140,804 + GFF2346 0.33 +0.3 140,804 + GFF2346 0.33 -2.7 140,805 - GFF2346 0.34 +0.5 140,856 + GFF2346 0.37 -0.0 140,904 - GFF2346 0.41 -0.4 140,996 + GFF2346 0.47 -0.1 140,997 - GFF2346 0.47 -0.0 141,143 - GFF2346 0.58 +0.5
Or see this region's nucleotide sequence