Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF222

Experiment: LB_plus_SM_buffer with P22_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF220 and GFF221 overlap by 4 nucleotidesGFF221 and GFF222 are separated by 29 nucleotidesGFF222 and GFF223 are separated by 256 nucleotides GFF220 - Citrate lyase beta chain (EC 4.1.3.6), at 218,221 to 219,090 GFF220 GFF221 - Citrate lyase beta chain (EC 4.1.3.6), at 219,087 to 219,380 GFF221 GFF222 - [Citrate [pro-3S]-lyase] ligase (EC 6.2.1.22), at 219,410 to 220,441 GFF222 GFF223 - Na(+)Citrate OH(-) antiporter, at 220,698 to 222,038 GFF223 Position (kb) 219 220 221Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 218.432 kb on - strand, within GFF220at 218.439 kb on + strand, within GFF220at 218.446 kb on - strand, within GFF220at 218.497 kb on - strand, within GFF220at 218.889 kb on + strand, within GFF220at 218.918 kb on + strand, within GFF220at 218.919 kb on - strand, within GFF220at 218.948 kb on - strand, within GFF220at 218.974 kb on + strand, within GFF220at 218.975 kb on - strand, within GFF220at 219.072 kb on + strandat 219.073 kb on - strandat 219.073 kb on - strandat 219.385 kb on + strandat 219.386 kb on - strandat 219.482 kb on + strandat 219.497 kb on - strandat 219.497 kb on - strandat 219.508 kb on - strandat 219.634 kb on - strand, within GFF222at 219.683 kb on - strand, within GFF222at 219.802 kb on + strand, within GFF222at 219.804 kb on + strand, within GFF222at 219.805 kb on - strand, within GFF222at 219.805 kb on - strand, within GFF222at 220.198 kb on + strand, within GFF222at 220.199 kb on - strand, within GFF222at 220.199 kb on - strand, within GFF222at 220.317 kb on + strand, within GFF222at 220.400 kb on + strandat 220.401 kb on - strandat 220.811 kb on - strandat 220.869 kb on - strand, within GFF223at 221.026 kb on + strand, within GFF223at 221.057 kb on + strand, within GFF223at 221.058 kb on - strand, within GFF223at 221.058 kb on - strand, within GFF223at 221.099 kb on + strand, within GFF223at 221.127 kb on + strand, within GFF223at 221.127 kb on + strand, within GFF223at 221.339 kb on + strand, within GFF223at 221.340 kb on - strand, within GFF223at 221.395 kb on + strand, within GFF223

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI
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218,432 - GFF220 0.24 -1.2
218,439 + GFF220 0.25 -0.7
218,446 - GFF220 0.26 -0.6
218,497 - GFF220 0.32 +0.1
218,889 + GFF220 0.77 -0.3
218,918 + GFF220 0.80 -0.4
218,919 - GFF220 0.80 +2.3
218,948 - GFF220 0.84 +0.1
218,974 + GFF220 0.87 -0.7
218,975 - GFF220 0.87 -0.8
219,072 + -0.5
219,073 - +0.4
219,073 - +0.4
219,385 + +0.7
219,386 - -0.8
219,482 + -3.2
219,497 - -0.5
219,497 - -0.9
219,508 - +0.2
219,634 - GFF222 0.22 +2.7
219,683 - GFF222 0.26 -1.5
219,802 + GFF222 0.38 +1.0
219,804 + GFF222 0.38 +1.8
219,805 - GFF222 0.38 +0.3
219,805 - GFF222 0.38 -0.7
220,198 + GFF222 0.76 +0.2
220,199 - GFF222 0.76 -0.8
220,199 - GFF222 0.76 -1.2
220,317 + GFF222 0.88 -0.9
220,400 + +1.1
220,401 - -0.1
220,811 - +0.8
220,869 - GFF223 0.13 -0.6
221,026 + GFF223 0.24 -2.6
221,057 + GFF223 0.27 +0.7
221,058 - GFF223 0.27 +1.4
221,058 - GFF223 0.27 +0.8
221,099 + GFF223 0.30 -0.3
221,127 + GFF223 0.32 -0.9
221,127 + GFF223 0.32 -0.3
221,339 + GFF223 0.48 +0.2
221,340 - GFF223 0.48 +1.1
221,395 + GFF223 0.52 -1.7

Or see this region's nucleotide sequence