Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2200 and GFF2201 are separated by 582 nucleotides GFF2201 and GFF2202 overlap by 10 nucleotides GFF2202 and GFF2203 are separated by 46 nucleotides GFF2203 and GFF2204 are separated by 2 nucleotides
GFF2200 - Phage CI-like repressor, at 617 to 823
GFF2200
GFF2201 - putative DNA-binding protein, at 1,406 to 1,726
GFF2201
GFF2202 - hypothetical protein, at 1,717 to 1,833
GFF2202
GFF2203 - Origin specific replication initiation factor #replication protein O, at 1,880 to 2,794
GFF2203
GFF2204 - DNA replication protein DnaC, at 2,797 to 3,546
GFF2204
Position (kb)
1
2 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 0.958 kb on + strand at 0.958 kb on + strand at 0.959 kb on - strand at 1.385 kb on - strand at 1.397 kb on + strand at 1.398 kb on - strand at 1.462 kb on - strand, within GFF2201 at 1.553 kb on - strand, within GFF2201 at 1.800 kb on + strand, within GFF2202 at 1.816 kb on + strand, within GFF2202 at 1.839 kb on - strand at 1.911 kb on + strand at 1.911 kb on + strand at 1.998 kb on - strand, within GFF2203 at 2.024 kb on - strand, within GFF2203 at 2.064 kb on + strand, within GFF2203 at 2.065 kb on - strand, within GFF2203 at 2.073 kb on + strand, within GFF2203 at 2.074 kb on - strand, within GFF2203 at 2.104 kb on + strand, within GFF2203 at 2.142 kb on - strand, within GFF2203 at 2.145 kb on + strand, within GFF2203 at 2.145 kb on + strand, within GFF2203 at 2.146 kb on - strand, within GFF2203 at 2.146 kb on - strand, within GFF2203 at 2.147 kb on - strand, within GFF2203 at 2.153 kb on - strand, within GFF2203 at 2.156 kb on - strand, within GFF2203 at 2.156 kb on - strand, within GFF2203 at 2.242 kb on + strand, within GFF2203 at 2.270 kb on + strand, within GFF2203 at 2.311 kb on - strand, within GFF2203 at 2.311 kb on - strand, within GFF2203 at 2.314 kb on + strand, within GFF2203 at 2.315 kb on - strand, within GFF2203 at 2.315 kb on - strand, within GFF2203 at 2.315 kb on - strand, within GFF2203 at 2.371 kb on + strand, within GFF2203 at 2.371 kb on + strand, within GFF2203 at 2.372 kb on - strand, within GFF2203 at 2.444 kb on - strand, within GFF2203 at 2.444 kb on - strand, within GFF2203 at 2.454 kb on + strand, within GFF2203 at 2.684 kb on + strand, within GFF2203 at 2.695 kb on - strand, within GFF2203
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 958 + +1.7 958 + -2.2 959 - -0.4 1,385 - -3.3 1,397 + -0.8 1,398 - +0.3 1,462 - GFF2201 0.17 +0.7 1,553 - GFF2201 0.46 +0.3 1,800 + GFF2202 0.71 -2.4 1,816 + GFF2202 0.85 -1.3 1,839 - +0.1 1,911 + +3.5 1,911 + +0.2 1,998 - GFF2203 0.13 -0.4 2,024 - GFF2203 0.16 +2.1 2,064 + GFF2203 0.20 -0.0 2,065 - GFF2203 0.20 +0.3 2,073 + GFF2203 0.21 -0.1 2,074 - GFF2203 0.21 -2.0 2,104 + GFF2203 0.24 +0.3 2,142 - GFF2203 0.29 -0.5 2,145 + GFF2203 0.29 +1.7 2,145 + GFF2203 0.29 +0.7 2,146 - GFF2203 0.29 +0.4 2,146 - GFF2203 0.29 -0.1 2,147 - GFF2203 0.29 +2.4 2,153 - GFF2203 0.30 +1.5 2,156 - GFF2203 0.30 +1.1 2,156 - GFF2203 0.30 -1.0 2,242 + GFF2203 0.40 -3.6 2,270 + GFF2203 0.43 +1.5 2,311 - GFF2203 0.47 -0.4 2,311 - GFF2203 0.47 -0.3 2,314 + GFF2203 0.47 +0.9 2,315 - GFF2203 0.48 -0.3 2,315 - GFF2203 0.48 -2.5 2,315 - GFF2203 0.48 +1.2 2,371 + GFF2203 0.54 -0.6 2,371 + GFF2203 0.54 +0.9 2,372 - GFF2203 0.54 -0.2 2,444 - GFF2203 0.62 +0.1 2,444 - GFF2203 0.62 +1.8 2,454 + GFF2203 0.63 +0.8 2,684 + GFF2203 0.88 -0.1 2,695 - GFF2203 0.89 -0.9
Or see this region's nucleotide sequence