Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2124 and GFF2125 are separated by 780 nucleotides GFF2125 and GFF2126 are separated by 26 nucleotides
GFF2124 - tRNA dihydrouridine synthase B (EC 1.-.-.-), at 10,683 to 11,528
GFF2124
GFF2125 - Ribosomal protein L11 methyltransferase (EC 2.1.1.-), at 12,309 to 13,175
GFF2125
GFF2126 - Pantothenate:Na+ symporter (TC 2.A.21.1.1), at 13,202 to 14,653
GFF2126
Position (kb)
12
13
14 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 11.327 kb on + strand, within GFF2124 at 11.369 kb on + strand, within GFF2124 at 11.396 kb on + strand, within GFF2124 at 11.397 kb on - strand, within GFF2124 at 11.493 kb on - strand at 11.561 kb on - strand at 11.667 kb on - strand at 11.689 kb on + strand at 11.693 kb on - strand at 11.907 kb on + strand at 11.913 kb on - strand at 12.106 kb on + strand at 12.109 kb on + strand at 12.109 kb on + strand at 12.110 kb on - strand at 12.110 kb on - strand at 12.110 kb on - strand at 12.113 kb on + strand at 12.149 kb on + strand at 12.150 kb on - strand at 12.310 kb on + strand at 12.419 kb on + strand, within GFF2125 at 12.420 kb on - strand, within GFF2125 at 12.504 kb on - strand, within GFF2125 at 12.553 kb on + strand, within GFF2125 at 12.612 kb on - strand, within GFF2125 at 12.751 kb on - strand, within GFF2125 at 12.887 kb on + strand, within GFF2125 at 13.317 kb on + strand at 13.328 kb on - strand at 13.328 kb on - strand at 13.348 kb on - strand, within GFF2126 at 13.349 kb on + strand, within GFF2126 at 13.349 kb on + strand, within GFF2126 at 13.529 kb on + strand, within GFF2126 at 13.534 kb on + strand, within GFF2126 at 13.535 kb on - strand, within GFF2126 at 13.549 kb on + strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.550 kb on - strand, within GFF2126 at 13.826 kb on - strand, within GFF2126 at 13.832 kb on - strand, within GFF2126 at 13.834 kb on + strand, within GFF2126 at 13.835 kb on - strand, within GFF2126 at 13.855 kb on + strand, within GFF2126 at 13.865 kb on - strand, within GFF2126 at 13.939 kb on + strand, within GFF2126 at 13.955 kb on + strand, within GFF2126 at 13.956 kb on - strand, within GFF2126 at 13.956 kb on - strand, within GFF2126 at 14.138 kb on - strand, within GFF2126 at 14.139 kb on + strand, within GFF2126
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 11,327 + GFF2124 0.76 -1.4 11,369 + GFF2124 0.81 -0.7 11,396 + GFF2124 0.84 -0.2 11,397 - GFF2124 0.84 -2.6 11,493 - +1.7 11,561 - +0.3 11,667 - +0.4 11,689 + +1.2 11,693 - -1.5 11,907 + +1.6 11,913 - +0.2 12,106 + +0.1 12,109 + -2.0 12,109 + +0.3 12,110 - +0.2 12,110 - +0.5 12,110 - +1.5 12,113 + -1.2 12,149 + -0.7 12,150 - +1.1 12,310 + -0.4 12,419 + GFF2125 0.13 -0.6 12,420 - GFF2125 0.13 +0.7 12,504 - GFF2125 0.22 +0.8 12,553 + GFF2125 0.28 +0.8 12,612 - GFF2125 0.35 -0.6 12,751 - GFF2125 0.51 -2.2 12,887 + GFF2125 0.67 -0.3 13,317 + +1.1 13,328 - +0.5 13,328 - -0.0 13,348 - GFF2126 0.10 +1.8 13,349 + GFF2126 0.10 -0.1 13,349 + GFF2126 0.10 -1.4 13,529 + GFF2126 0.23 -0.3 13,534 + GFF2126 0.23 -0.1 13,535 - GFF2126 0.23 -0.2 13,549 + GFF2126 0.24 +0.4 13,550 - GFF2126 0.24 -0.2 13,550 - GFF2126 0.24 +0.5 13,550 - GFF2126 0.24 -0.5 13,826 - GFF2126 0.43 -0.4 13,832 - GFF2126 0.43 +0.1 13,834 + GFF2126 0.44 -0.4 13,835 - GFF2126 0.44 -0.1 13,855 + GFF2126 0.45 -0.5 13,865 - GFF2126 0.46 -0.5 13,939 + GFF2126 0.51 +3.5 13,955 + GFF2126 0.52 -3.6 13,956 - GFF2126 0.52 +1.3 13,956 - GFF2126 0.52 -1.2 14,138 - GFF2126 0.64 -1.3 14,139 + GFF2126 0.65 -0.4
Or see this region's nucleotide sequence