Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2078 and GFF2079 are separated by 11 nucleotides GFF2079 and GFF2080 are separated by 100 nucleotides GFF2080 and GFF2081 are separated by 40 nucleotides
GFF2078 - Acetaldehyde dehydrogenase, ethanolamine utilization cluster (EC 1.2.1.10), at 41,416 to 42,819
GFF2078
GFF2079 - Ethanolamine utilization polyhedral-body-like protein EutN, at 42,831 to 43,130
GFF2079
GFF2080 - Ethanolamine utilization polyhedral-body-like protein EutM, at 43,231 to 43,521
GFF2080
GFF2081 - Phosphate acetyltransferase (EC 2.3.1.8), ethanolamine utilization-specific, at 43,562 to 44,578
GFF2081
Position (kb) 43
44 Strain fitness (log2 ratio) -2
-1
0
1
2 at 42.833 kb on - strand at 42.833 kb on - strand at 42.837 kb on - strand at 43.086 kb on + strand, within GFF2079 at 43.086 kb on + strand, within GFF2079 at 43.398 kb on + strand, within GFF2080 at 43.398 kb on + strand, within GFF2080 at 43.454 kb on - strand, within GFF2080 at 43.580 kb on + strand at 43.643 kb on - strand at 44.073 kb on - strand, within GFF2081 at 44.093 kb on + strand, within GFF2081 at 44.110 kb on - strand, within GFF2081 at 44.222 kb on - strand, within GFF2081 at 44.222 kb on - strand, within GFF2081 at 44.401 kb on + strand, within GFF2081 at 44.401 kb on + strand, within GFF2081
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 42,833 - +0.2 42,833 - +1.2 42,837 - +1.1 43,086 + GFF2079 0.85 -0.9 43,086 + GFF2079 0.85 +0.6 43,398 + GFF2080 0.57 -0.9 43,398 + GFF2080 0.57 -0.2 43,454 - GFF2080 0.77 -1.6 43,580 + -1.1 43,643 - -0.4 44,073 - GFF2081 0.50 -1.0 44,093 + GFF2081 0.52 -2.4 44,110 - GFF2081 0.54 -0.8 44,222 - GFF2081 0.65 +1.0 44,222 - GFF2081 0.65 -0.4 44,401 + GFF2081 0.82 -0.7 44,401 + GFF2081 0.82 +1.8
Or see this region's nucleotide sequence