Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF203 and GFF204 are separated by 15 nucleotides GFF204 and GFF205 are separated by 67 nucleotides GFF205 and GFF206 are separated by 227 nucleotides
GFF203 - Electron transfer flavoprotein, alpha subunit FixB, at 198,679 to 199,620
GFF203
GFF204 - Electron transfer flavoprotein, beta subunit FixA, at 199,636 to 200,406
GFF204
GFF205 - FIG01045916: hypothetical protein, at 200,474 to 200,659
GFF205
GFF206 - L-carnitine/gamma-butyrobetaine antiporter, at 200,887 to 202,404
GFF206
Position (kb) 199
200
201 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 198.653 kb on + strand at 198.654 kb on - strand at 198.854 kb on + strand, within GFF203 at 198.855 kb on - strand, within GFF203 at 199.245 kb on + strand, within GFF203 at 199.421 kb on + strand, within GFF203 at 199.540 kb on + strand at 199.541 kb on - strand at 199.638 kb on - strand at 199.760 kb on - strand, within GFF204 at 199.855 kb on - strand, within GFF204 at 199.931 kb on - strand, within GFF204 at 200.058 kb on + strand, within GFF204 at 200.059 kb on - strand, within GFF204 at 200.063 kb on - strand, within GFF204 at 200.770 kb on + strand at 201.022 kb on + strand at 201.022 kb on + strand at 201.022 kb on + strand at 201.058 kb on + strand, within GFF206 at 201.058 kb on + strand, within GFF206 at 201.060 kb on + strand, within GFF206 at 201.224 kb on - strand, within GFF206 at 201.224 kb on - strand, within GFF206 at 201.226 kb on + strand, within GFF206 at 201.227 kb on - strand, within GFF206 at 201.229 kb on + strand, within GFF206 at 201.230 kb on - strand, within GFF206 at 201.230 kb on - strand, within GFF206 at 201.231 kb on + strand, within GFF206 at 201.232 kb on - strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.334 kb on + strand, within GFF206 at 201.335 kb on - strand, within GFF206 at 201.337 kb on - strand, within GFF206
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 198,653 + -0.4 198,654 - +2.3 198,854 + GFF203 0.19 +0.4 198,855 - GFF203 0.19 +0.6 199,245 + GFF203 0.60 -3.9 199,421 + GFF203 0.79 +1.7 199,540 + -2.6 199,541 - -2.6 199,638 - +0.1 199,760 - GFF204 0.16 +0.1 199,855 - GFF204 0.28 -1.0 199,931 - GFF204 0.38 -1.5 200,058 + GFF204 0.55 -1.2 200,059 - GFF204 0.55 -0.5 200,063 - GFF204 0.55 -0.8 200,770 + +0.3 201,022 + +1.2 201,022 + -1.4 201,022 + -0.1 201,058 + GFF206 0.11 -0.5 201,058 + GFF206 0.11 -0.3 201,060 + GFF206 0.11 -1.4 201,224 - GFF206 0.22 -2.1 201,224 - GFF206 0.22 -0.4 201,226 + GFF206 0.22 -1.0 201,227 - GFF206 0.22 -2.2 201,229 + GFF206 0.23 -0.5 201,230 - GFF206 0.23 +0.5 201,230 - GFF206 0.23 +0.5 201,231 + GFF206 0.23 +0.5 201,232 - GFF206 0.23 +0.4 201,334 + GFF206 0.29 -1.0 201,334 + GFF206 0.29 +1.0 201,334 + GFF206 0.29 +1.1 201,335 - GFF206 0.30 +0.1 201,337 - GFF206 0.30 -3.0
Or see this region's nucleotide sequence