Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1984 and GFF1985 are separated by 42 nucleotides GFF1985 and GFF1986 are separated by 42 nucleotides GFF1986 and GFF1987 are separated by 42 nucleotides GFF1987 and GFF1980 are separated by 236 nucleotides GFF1980 and GFF1981 are separated by 1 nucleotides GFF1981 and GFF1982 are separated by 53 nucleotides
GFF1984 - tRNA-Ala-GGC, at 175,328 to 175,403
GFF1984
GFF1985 - tRNA-Ala-GGC, at 175,446 to 175,521
GFF1985
GFF1986 - tRNA-Ala-GGC, at 175,564 to 175,639
GFF1986
GFF1987 - tRNA-Ala-GGC, at 175,682 to 175,757
GFF1987
GFF1980 - Putative negative regulator, at 175,994 to 176,341
GFF1980
GFF1981 - FIG00638165: hypothetical protein, at 176,343 to 176,735
GFF1981
GFF1982 - Glutamyl-tRNA synthetase (EC 6.1.1.17), at 176,789 to 178,204
GFF1982
Position (kb) 176
177 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 175.456 kb on - strand, within GFF1985 at 175.456 kb on - strand, within GFF1985 at 175.979 kb on - strand at 176.145 kb on - strand, within GFF1980 at 176.319 kb on + strand at 176.340 kb on - strand at 176.492 kb on + strand, within GFF1981 at 176.718 kb on - strand at 176.741 kb on - strand at 176.770 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 175,456 - GFF1985 0.13 -0.4 175,456 - GFF1985 0.13 -2.6 175,979 - -1.9 176,145 - GFF1980 0.43 -1.8 176,319 + -2.1 176,340 - -0.0 176,492 + GFF1981 0.38 -1.9 176,718 - -3.1 176,741 - +0.0 176,770 + -0.9
Or see this region's nucleotide sequence