Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1972 and GFF1973 are separated by 203 nucleotides GFF1973 and GFF1974 are separated by 19 nucleotides
GFF1972 - Glucokinase (EC 2.7.1.2), at 164,354 to 165,319
GFF1972
GFF1973 - Chloride channel protein, at 165,523 to 166,758
GFF1973
GFF1974 - Pyruvate decarboxylase (EC 4.1.1.1); Alpha-keto-acid decarboxylase (EC 4.1.1.-), at 166,778 to 168,430
GFF1974
Position (kb) 165
166
167 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 164.542 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972 at 164.544 kb on + strand, within GFF1972 at 164.824 kb on + strand, within GFF1972 at 164.825 kb on - strand, within GFF1972 at 165.132 kb on - strand, within GFF1972 at 165.290 kb on + strand at 165.291 kb on - strand at 165.303 kb on - strand at 165.303 kb on - strand at 165.309 kb on + strand at 165.310 kb on - strand at 165.416 kb on + strand at 165.419 kb on - strand at 165.493 kb on - strand at 165.493 kb on - strand at 165.498 kb on + strand at 165.498 kb on + strand at 165.522 kb on + strand at 165.523 kb on - strand at 165.698 kb on + strand, within GFF1973 at 165.705 kb on + strand, within GFF1973 at 165.706 kb on - strand, within GFF1973 at 165.842 kb on + strand, within GFF1973 at 165.867 kb on - strand, within GFF1973 at 165.900 kb on - strand, within GFF1973 at 166.320 kb on + strand, within GFF1973 at 166.322 kb on + strand, within GFF1973 at 166.428 kb on + strand, within GFF1973 at 166.429 kb on - strand, within GFF1973 at 166.970 kb on + strand, within GFF1974 at 166.971 kb on - strand, within GFF1974 at 167.002 kb on + strand, within GFF1974 at 167.003 kb on - strand, within GFF1974 at 167.037 kb on - strand, within GFF1974 at 167.041 kb on + strand, within GFF1974 at 167.044 kb on - strand, within GFF1974 at 167.228 kb on + strand, within GFF1974 at 167.322 kb on + strand, within GFF1974 at 167.330 kb on + strand, within GFF1974 at 167.446 kb on + strand, within GFF1974 at 167.590 kb on + strand, within GFF1974 at 167.609 kb on + strand, within GFF1974 at 167.610 kb on - strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.702 kb on + strand, within GFF1974
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 164,542 + GFF1972 0.19 -1.8 164,544 + GFF1972 0.20 -1.2 164,544 + GFF1972 0.20 -2.0 164,824 + GFF1972 0.49 +0.4 164,825 - GFF1972 0.49 -1.0 165,132 - GFF1972 0.81 +0.6 165,290 + -1.0 165,291 - -1.3 165,303 - -1.5 165,303 - -1.4 165,309 + -0.4 165,310 - -0.5 165,416 + -1.1 165,419 - -0.7 165,493 - -2.8 165,493 - -2.8 165,498 + -2.4 165,498 + +0.0 165,522 + -1.3 165,523 - -0.4 165,698 + GFF1973 0.14 -4.2 165,705 + GFF1973 0.15 +1.3 165,706 - GFF1973 0.15 -0.4 165,842 + GFF1973 0.26 -1.0 165,867 - GFF1973 0.28 -1.7 165,900 - GFF1973 0.31 -0.8 166,320 + GFF1973 0.64 +1.0 166,322 + GFF1973 0.65 -1.2 166,428 + GFF1973 0.73 +0.2 166,429 - GFF1973 0.73 -1.2 166,970 + GFF1974 0.12 +2.1 166,971 - GFF1974 0.12 -1.5 167,002 + GFF1974 0.14 -1.1 167,003 - GFF1974 0.14 -1.0 167,037 - GFF1974 0.16 +0.3 167,041 + GFF1974 0.16 -1.4 167,044 - GFF1974 0.16 -2.8 167,228 + GFF1974 0.27 -3.0 167,322 + GFF1974 0.33 -1.1 167,330 + GFF1974 0.33 +0.3 167,446 + GFF1974 0.40 -3.0 167,590 + GFF1974 0.49 +2.1 167,609 + GFF1974 0.50 +2.1 167,610 - GFF1974 0.50 -0.7 167,649 + GFF1974 0.53 +2.4 167,649 + GFF1974 0.53 -0.0 167,702 + GFF1974 0.56 -1.0
Or see this region's nucleotide sequence