Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1966 and GFF1967 are separated by 160 nucleotides GFF1967 and GFF1968 are separated by 247 nucleotides GFF1968 and GFF1969 are separated by 114 nucleotides GFF1969 and GFF1970 are separated by 286 nucleotides
GFF1966 - FIG00638164: hypothetical protein, at 160,187 to 160,429
GFF1966
GFF1967 - hypothetical protein, at 160,590 to 160,703
GFF1967
GFF1968 - Lipid A biosynthesis lauroyl acyltransferase (EC 2.3.1.-), at 160,951 to 161,871
GFF1968
GFF1969 - hypothetical protein, at 161,986 to 162,108
GFF1969
GFF1970 - Uncharacterized PLP-dependent aminotransferase YfdZ, at 162,395 to 163,633
GFF1970
Position (kb)
160
161
162 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 159.962 kb on - strand at 159.977 kb on - strand at 159.977 kb on - strand at 160.177 kb on + strand at 160.181 kb on + strand at 160.182 kb on - strand at 160.289 kb on + strand, within GFF1966 at 160.377 kb on + strand, within GFF1966 at 160.419 kb on - strand at 160.495 kb on - strand at 160.689 kb on + strand, within GFF1967 at 160.738 kb on + strand at 160.791 kb on - strand at 160.794 kb on + strand at 160.795 kb on - strand at 160.836 kb on + strand at 160.836 kb on + strand at 160.837 kb on - strand at 161.627 kb on - strand, within GFF1968 at 161.630 kb on + strand, within GFF1968 at 161.707 kb on + strand, within GFF1968 at 161.708 kb on - strand, within GFF1968 at 161.710 kb on - strand, within GFF1968 at 161.710 kb on - strand, within GFF1968 at 161.917 kb on + strand at 162.113 kb on - strand at 162.216 kb on - strand at 162.225 kb on + strand at 162.248 kb on - strand at 162.343 kb on + strand at 162.519 kb on - strand, within GFF1970 at 162.519 kb on - strand, within GFF1970 at 162.519 kb on - strand, within GFF1970 at 162.621 kb on + strand, within GFF1970 at 162.778 kb on + strand, within GFF1970 at 162.811 kb on - strand, within GFF1970 at 162.827 kb on - strand, within GFF1970
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 159,962 - -0.2 159,977 - -0.7 159,977 - -2.1 160,177 + -1.0 160,181 + -0.2 160,182 - -1.0 160,289 + GFF1966 0.42 -1.1 160,377 + GFF1966 0.78 -0.4 160,419 - -0.4 160,495 - -0.1 160,689 + GFF1967 0.87 -1.4 160,738 + -2.4 160,791 - -0.1 160,794 + +0.3 160,795 - +1.1 160,836 + -0.5 160,836 + +0.3 160,837 - -1.2 161,627 - GFF1968 0.73 -2.5 161,630 + GFF1968 0.74 -1.6 161,707 + GFF1968 0.82 -1.0 161,708 - GFF1968 0.82 -0.2 161,710 - GFF1968 0.82 -0.3 161,710 - GFF1968 0.82 -1.0 161,917 + -0.9 162,113 - +0.5 162,216 - -0.2 162,225 + -0.7 162,248 - -1.3 162,343 + -1.2 162,519 - GFF1970 0.10 -0.2 162,519 - GFF1970 0.10 -0.8 162,519 - GFF1970 0.10 -0.5 162,621 + GFF1970 0.18 -3.1 162,778 + GFF1970 0.31 -0.3 162,811 - GFF1970 0.34 +0.2 162,827 - GFF1970 0.35 -0.7
Or see this region's nucleotide sequence