Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1963 and GFF1964 are separated by 13 nucleotides GFF1964 and GFF1965 are separated by 131 nucleotides GFF1965 and GFF1966 are separated by 275 nucleotides GFF1966 and GFF1967 are separated by 160 nucleotides
GFF1963 - Phosphoglycerate transport regulatory protein PgtC, at 156,891 to 158,114
GFF1963
GFF1964 - FIG01047539: hypothetical protein, at 158,128 to 158,388
GFF1964
GFF1965 - Phosphoglycerate transporter protein PgtP, at 158,520 to 159,911
GFF1965
GFF1966 - FIG00638164: hypothetical protein, at 160,187 to 160,429
GFF1966
GFF1967 - hypothetical protein, at 160,590 to 160,703
GFF1967
Position (kb) 158
159
160 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 157.930 kb on - strand, within GFF1963 at 157.953 kb on - strand, within GFF1963 at 158.063 kb on - strand at 158.103 kb on - strand at 158.586 kb on + strand at 158.602 kb on + strand at 158.735 kb on + strand, within GFF1965 at 158.736 kb on - strand, within GFF1965 at 158.742 kb on - strand, within GFF1965 at 158.767 kb on + strand, within GFF1965 at 158.942 kb on + strand, within GFF1965 at 159.005 kb on + strand, within GFF1965 at 159.087 kb on + strand, within GFF1965 at 159.089 kb on + strand, within GFF1965 at 159.270 kb on - strand, within GFF1965 at 159.270 kb on - strand, within GFF1965 at 159.273 kb on + strand, within GFF1965 at 159.300 kb on + strand, within GFF1965 at 159.327 kb on + strand, within GFF1965 at 159.350 kb on + strand, within GFF1965 at 159.350 kb on + strand, within GFF1965 at 159.362 kb on + strand, within GFF1965 at 159.366 kb on + strand, within GFF1965 at 159.595 kb on + strand, within GFF1965 at 159.595 kb on + strand, within GFF1965 at 159.704 kb on + strand, within GFF1965 at 159.871 kb on + strand at 159.875 kb on + strand at 159.876 kb on - strand at 159.962 kb on - strand at 159.977 kb on - strand at 159.977 kb on - strand at 160.177 kb on + strand at 160.181 kb on + strand at 160.182 kb on - strand at 160.289 kb on + strand, within GFF1966 at 160.377 kb on + strand, within GFF1966 at 160.419 kb on - strand at 160.495 kb on - strand at 160.689 kb on + strand, within GFF1967 at 160.738 kb on + strand at 160.791 kb on - strand at 160.794 kb on + strand at 160.795 kb on - strand at 160.836 kb on + strand at 160.836 kb on + strand at 160.837 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 157,930 - GFF1963 0.85 -1.0 157,953 - GFF1963 0.87 +1.4 158,063 - -1.3 158,103 - -2.4 158,586 + -1.1 158,602 + -0.9 158,735 + GFF1965 0.15 -1.7 158,736 - GFF1965 0.16 +0.4 158,742 - GFF1965 0.16 -2.8 158,767 + GFF1965 0.18 -0.2 158,942 + GFF1965 0.30 -0.1 159,005 + GFF1965 0.35 -3.5 159,087 + GFF1965 0.41 -0.3 159,089 + GFF1965 0.41 -0.7 159,270 - GFF1965 0.54 -0.8 159,270 - GFF1965 0.54 +0.0 159,273 + GFF1965 0.54 -1.2 159,300 + GFF1965 0.56 -1.0 159,327 + GFF1965 0.58 -0.4 159,350 + GFF1965 0.60 -0.9 159,350 + GFF1965 0.60 -0.1 159,362 + GFF1965 0.60 -2.8 159,366 + GFF1965 0.61 -0.8 159,595 + GFF1965 0.77 +0.3 159,595 + GFF1965 0.77 -2.0 159,704 + GFF1965 0.85 -1.1 159,871 + -2.9 159,875 + -2.8 159,876 - +0.2 159,962 - -0.2 159,977 - -0.7 159,977 - -2.1 160,177 + -1.0 160,181 + -0.2 160,182 - -1.0 160,289 + GFF1966 0.42 -1.1 160,377 + GFF1966 0.78 -0.4 160,419 - -0.4 160,495 - -0.1 160,689 + GFF1967 0.87 -1.4 160,738 + -2.4 160,791 - -0.1 160,794 + +0.3 160,795 - +1.1 160,836 + -0.5 160,836 + +0.3 160,837 - -1.2
Or see this region's nucleotide sequence