Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1961 and GFF1962 overlap by 11 nucleotides GFF1962 and GFF1963 overlap by 4 nucleotides
GFF1961 - Phosphoglycerate transport system transcriptional regulatory protein PgtA, at 153,651 to 154,898
GFF1961
GFF1962 - Phosphoglycerate transport system sensor protein PgtB (EC 2.7.3.-), at 154,888 to 156,894
GFF1962
GFF1963 - Phosphoglycerate transport regulatory protein PgtC, at 156,891 to 158,114
GFF1963
Position (kb) 154
155
156
157 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 154.088 kb on - strand, within GFF1961 at 154.302 kb on - strand, within GFF1961 at 154.315 kb on - strand, within GFF1961 at 154.315 kb on - strand, within GFF1961 at 154.370 kb on + strand, within GFF1961 at 154.371 kb on - strand, within GFF1961 at 154.371 kb on - strand, within GFF1961 at 154.650 kb on - strand, within GFF1961 at 154.724 kb on + strand, within GFF1961 at 154.794 kb on - strand at 154.849 kb on - strand at 155.057 kb on - strand at 155.106 kb on - strand, within GFF1962 at 155.229 kb on + strand, within GFF1962 at 155.336 kb on + strand, within GFF1962 at 155.485 kb on + strand, within GFF1962 at 155.555 kb on - strand, within GFF1962 at 155.755 kb on + strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.756 kb on - strand, within GFF1962 at 155.761 kb on + strand, within GFF1962 at 155.868 kb on + strand, within GFF1962 at 155.935 kb on - strand, within GFF1962 at 155.941 kb on + strand, within GFF1962 at 155.942 kb on - strand, within GFF1962 at 155.980 kb on - strand, within GFF1962 at 156.112 kb on - strand, within GFF1962 at 156.279 kb on + strand, within GFF1962 at 156.280 kb on - strand, within GFF1962 at 156.281 kb on + strand, within GFF1962 at 156.282 kb on - strand, within GFF1962 at 156.283 kb on + strand, within GFF1962 at 156.284 kb on - strand, within GFF1962 at 156.284 kb on - strand, within GFF1962 at 156.492 kb on - strand, within GFF1962 at 156.613 kb on - strand, within GFF1962 at 156.625 kb on + strand, within GFF1962 at 156.637 kb on + strand, within GFF1962 at 156.842 kb on - strand at 156.984 kb on - strand at 157.050 kb on + strand, within GFF1963 at 157.051 kb on - strand, within GFF1963 at 157.167 kb on + strand, within GFF1963 at 157.323 kb on + strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.324 kb on - strand, within GFF1963 at 157.327 kb on - strand, within GFF1963 at 157.421 kb on + strand, within GFF1963 at 157.457 kb on + strand, within GFF1963 at 157.459 kb on + strand, within GFF1963 at 157.460 kb on - strand, within GFF1963
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 154,088 - GFF1961 0.35 -2.4 154,302 - GFF1961 0.52 -3.6 154,315 - GFF1961 0.53 -0.2 154,315 - GFF1961 0.53 +0.0 154,370 + GFF1961 0.58 -1.7 154,371 - GFF1961 0.58 -0.8 154,371 - GFF1961 0.58 -2.6 154,650 - GFF1961 0.80 +0.2 154,724 + GFF1961 0.86 -1.2 154,794 - -0.2 154,849 - -0.1 155,057 - -2.0 155,106 - GFF1962 0.11 +0.3 155,229 + GFF1962 0.17 -0.6 155,336 + GFF1962 0.22 -0.9 155,485 + GFF1962 0.30 -2.4 155,555 - GFF1962 0.33 -3.9 155,755 + GFF1962 0.43 -2.4 155,756 - GFF1962 0.43 +0.0 155,756 - GFF1962 0.43 -1.7 155,756 - GFF1962 0.43 -1.4 155,761 + GFF1962 0.43 -0.2 155,868 + GFF1962 0.49 -1.9 155,935 - GFF1962 0.52 -2.4 155,941 + GFF1962 0.52 -2.6 155,942 - GFF1962 0.53 -0.6 155,980 - GFF1962 0.54 -0.9 156,112 - GFF1962 0.61 -3.8 156,279 + GFF1962 0.69 -1.1 156,280 - GFF1962 0.69 -2.4 156,281 + GFF1962 0.69 -2.4 156,282 - GFF1962 0.69 -1.7 156,283 + GFF1962 0.70 +0.6 156,284 - GFF1962 0.70 -0.9 156,284 - GFF1962 0.70 +0.1 156,492 - GFF1962 0.80 +0.0 156,613 - GFF1962 0.86 +1.2 156,625 + GFF1962 0.87 -0.4 156,637 + GFF1962 0.87 -1.4 156,842 - -2.6 156,984 - -0.4 157,050 + GFF1963 0.13 +1.0 157,051 - GFF1963 0.13 +0.8 157,167 + GFF1963 0.23 -0.1 157,323 + GFF1963 0.35 -0.4 157,324 - GFF1963 0.35 -0.0 157,324 - GFF1963 0.35 -0.3 157,324 - GFF1963 0.35 -2.6 157,327 - GFF1963 0.36 -1.5 157,421 + GFF1963 0.43 +0.5 157,457 + GFF1963 0.46 -2.2 157,459 + GFF1963 0.46 +0.0 157,460 - GFF1963 0.46 -0.5
Or see this region's nucleotide sequence