Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF196

Experiment: LB_plus_SM_buffer with P22_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF194 and GFF195 overlap by 8 nucleotidesGFF195 and GFF196 are separated by 406 nucleotidesGFF196 and GFF197 are separated by 897 nucleotides GFF194 - Glutathione-regulated potassium-efflux system protein KefC, at 188,450 to 190,312 GFF194 GFF195 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 190,305 to 190,835 GFF195 GFF196 - Choline-sulfatase (EC 3.1.6.6), at 191,242 to 193,131 GFF196 GFF197 - Probable secreted protein, at 194,029 to 194,319 GFF197 Position (kb) 191 192 193 194Strain fitness (log2 ratio) -2 -1 0 1 2at 190.300 kb on + strandat 190.300 kb on + strandat 190.300 kb on + strandat 190.301 kb on - strandat 190.336 kb on - strandat 190.346 kb on + strandat 190.347 kb on - strandat 190.740 kb on + strand, within GFF195at 190.741 kb on - strand, within GFF195at 190.819 kb on + strandat 190.820 kb on - strandat 190.897 kb on - strandat 191.041 kb on + strandat 191.042 kb on - strandat 191.113 kb on - strandat 191.122 kb on - strandat 191.145 kb on + strandat 191.998 kb on + strand, within GFF196at 192.501 kb on + strand, within GFF196at 192.653 kb on + strand, within GFF196at 192.665 kb on + strand, within GFF196at 192.684 kb on + strand, within GFF196at 192.783 kb on - strand, within GFF196at 192.933 kb on + strand, within GFF196at 192.935 kb on + strand, within GFF196at 193.495 kb on - strandat 193.710 kb on + strandat 193.823 kb on + strandat 193.827 kb on + strandat 193.967 kb on - strandat 194.058 kb on - strand, within GFF197at 194.058 kb on - strand, within GFF197

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI
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190,300 + -0.4
190,300 + +0.1
190,300 + -0.3
190,301 - -0.2
190,336 - +0.6
190,346 + -1.5
190,347 - -1.7
190,740 + GFF195 0.82 +0.3
190,741 - GFF195 0.82 -0.5
190,819 + +1.7
190,820 - +1.1
190,897 - -1.2
191,041 + -0.9
191,042 - -1.3
191,113 - -2.2
191,122 - +0.8
191,145 + -0.3
191,998 + GFF196 0.40 -0.1
192,501 + GFF196 0.67 -0.8
192,653 + GFF196 0.75 -0.1
192,665 + GFF196 0.75 -2.2
192,684 + GFF196 0.76 -2.5
192,783 - GFF196 0.82 -0.3
192,933 + GFF196 0.89 -0.6
192,935 + GFF196 0.90 -0.8
193,495 - +0.4
193,710 + +1.2
193,823 + -0.3
193,827 + -0.1
193,967 - -0.4
194,058 - GFF197 0.10 -0.7
194,058 - GFF197 0.10 +1.9

Or see this region's nucleotide sequence