Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF194 and GFF195 overlap by 8 nucleotides GFF195 and GFF196 are separated by 406 nucleotides GFF196 and GFF197 are separated by 897 nucleotides
GFF194 - Glutathione-regulated potassium-efflux system protein KefC, at 188,450 to 190,312
GFF194
GFF195 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 190,305 to 190,835
GFF195
GFF196 - Choline-sulfatase (EC 3.1.6.6), at 191,242 to 193,131
GFF196
GFF197 - Probable secreted protein, at 194,029 to 194,319
GFF197
Position (kb)
191
192
193
194 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 190.300 kb on + strand at 190.300 kb on + strand at 190.300 kb on + strand at 190.301 kb on - strand at 190.336 kb on - strand at 190.346 kb on + strand at 190.347 kb on - strand at 190.740 kb on + strand, within GFF195 at 190.741 kb on - strand, within GFF195 at 190.819 kb on + strand at 190.820 kb on - strand at 190.897 kb on - strand at 191.041 kb on + strand at 191.042 kb on - strand at 191.113 kb on - strand at 191.122 kb on - strand at 191.145 kb on + strand at 191.998 kb on + strand, within GFF196 at 192.501 kb on + strand, within GFF196 at 192.653 kb on + strand, within GFF196 at 192.665 kb on + strand, within GFF196 at 192.684 kb on + strand, within GFF196 at 192.783 kb on - strand, within GFF196 at 192.933 kb on + strand, within GFF196 at 192.935 kb on + strand, within GFF196 at 193.495 kb on - strand at 193.710 kb on + strand at 193.823 kb on + strand at 193.827 kb on + strand at 193.967 kb on - strand at 194.058 kb on - strand, within GFF197 at 194.058 kb on - strand, within GFF197
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 190,300 + -0.4 190,300 + +0.1 190,300 + -0.3 190,301 - -0.2 190,336 - +0.6 190,346 + -1.5 190,347 - -1.7 190,740 + GFF195 0.82 +0.3 190,741 - GFF195 0.82 -0.5 190,819 + +1.7 190,820 - +1.1 190,897 - -1.2 191,041 + -0.9 191,042 - -1.3 191,113 - -2.2 191,122 - +0.8 191,145 + -0.3 191,998 + GFF196 0.40 -0.1 192,501 + GFF196 0.67 -0.8 192,653 + GFF196 0.75 -0.1 192,665 + GFF196 0.75 -2.2 192,684 + GFF196 0.76 -2.5 192,783 - GFF196 0.82 -0.3 192,933 + GFF196 0.89 -0.6 192,935 + GFF196 0.90 -0.8 193,495 - +0.4 193,710 + +1.2 193,823 + -0.3 193,827 + -0.1 193,967 - -0.4 194,058 - GFF197 0.10 -0.7 194,058 - GFF197 0.10 +1.9
Or see this region's nucleotide sequence