Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1950 and GFF1951 are separated by 35 nucleotides GFF1951 and GFF1952 are separated by 165 nucleotides GFF1952 and GFF1953 are separated by 99 nucleotides
GFF1950 - Chorismate synthase (EC 4.2.3.5), at 140,931 to 142,016
GFF1950
GFF1951 - Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p, at 142,052 to 142,984
GFF1951
GFF1952 - FIG001674: hypothetical protein, at 143,150 to 143,701
GFF1952
GFF1953 - Phosphohistidine phosphatase SixA, at 143,801 to 144,187
GFF1953
Position (kb) 142
143 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 141.135 kb on - strand, within GFF1950 at 141.685 kb on + strand, within GFF1950 at 141.686 kb on - strand, within GFF1950 at 141.686 kb on - strand, within GFF1950 at 141.851 kb on + strand, within GFF1950 at 141.942 kb on - strand at 142.063 kb on - strand at 142.185 kb on - strand, within GFF1951 at 142.234 kb on - strand, within GFF1951 at 142.356 kb on + strand, within GFF1951 at 142.367 kb on - strand, within GFF1951 at 142.384 kb on - strand, within GFF1951 at 142.441 kb on + strand, within GFF1951 at 142.462 kb on + strand, within GFF1951 at 142.530 kb on + strand, within GFF1951 at 142.531 kb on - strand, within GFF1951 at 142.824 kb on + strand, within GFF1951 at 142.942 kb on + strand at 143.045 kb on + strand at 143.045 kb on + strand at 143.289 kb on + strand, within GFF1952 at 143.351 kb on - strand, within GFF1952 at 143.369 kb on + strand, within GFF1952 at 143.369 kb on + strand, within GFF1952 at 143.558 kb on - strand, within GFF1952 at 143.694 kb on + strand at 143.879 kb on - strand, within GFF1953
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 141,135 - GFF1950 0.19 -1.0 141,685 + GFF1950 0.69 +0.1 141,686 - GFF1950 0.70 +1.2 141,686 - GFF1950 0.70 -0.7 141,851 + GFF1950 0.85 -2.5 141,942 - -2.6 142,063 - -1.5 142,185 - GFF1951 0.14 -1.4 142,234 - GFF1951 0.20 -0.7 142,356 + GFF1951 0.33 -1.3 142,367 - GFF1951 0.34 -0.6 142,384 - GFF1951 0.36 -0.4 142,441 + GFF1951 0.42 -0.9 142,462 + GFF1951 0.44 -1.2 142,530 + GFF1951 0.51 +0.0 142,531 - GFF1951 0.51 -1.4 142,824 + GFF1951 0.83 -3.1 142,942 + -0.2 143,045 + -1.3 143,045 + +0.8 143,289 + GFF1952 0.25 -0.1 143,351 - GFF1952 0.36 -1.4 143,369 + GFF1952 0.40 -0.9 143,369 + GFF1952 0.40 -2.0 143,558 - GFF1952 0.74 -1.8 143,694 + -2.0 143,879 - GFF1953 0.20 +0.8
Or see this region's nucleotide sequence