Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF192 and GFF193 are separated by 105 nucleotides GFF193 and GFF194 are separated by 198 nucleotides GFF194 and GFF195 overlap by 8 nucleotides GFF195 and GFF196 are separated by 406 nucleotides
GFF192 - 'Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41)' transl_table=11, at 186,818 to 187,666
GFF192
GFF193 - Dihydrofolate reductase (EC 1.5.1.3), at 187,772 to 188,251
GFF193
GFF194 - Glutathione-regulated potassium-efflux system protein KefC, at 188,450 to 190,312
GFF194
GFF195 - Glutathione-regulated potassium-efflux system ancillary protein KefF, at 190,305 to 190,835
GFF195
GFF196 - Choline-sulfatase (EC 3.1.6.6), at 191,242 to 193,131
GFF196
Position (kb) 188
189
190
191 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 187.478 kb on + strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.479 kb on - strand, within GFF192 at 187.605 kb on - strand at 187.605 kb on - strand at 188.288 kb on - strand at 188.391 kb on - strand at 188.391 kb on - strand at 188.414 kb on + strand at 188.415 kb on - strand at 188.805 kb on + strand, within GFF194 at 188.996 kb on - strand, within GFF194 at 189.011 kb on - strand, within GFF194 at 189.011 kb on - strand, within GFF194 at 189.261 kb on - strand, within GFF194 at 189.317 kb on + strand, within GFF194 at 189.318 kb on - strand, within GFF194 at 189.997 kb on + strand, within GFF194 at 189.998 kb on - strand, within GFF194 at 190.055 kb on + strand, within GFF194 at 190.241 kb on + strand at 190.300 kb on + strand at 190.300 kb on + strand at 190.300 kb on + strand at 190.301 kb on - strand at 190.336 kb on - strand at 190.346 kb on + strand at 190.347 kb on - strand at 190.740 kb on + strand, within GFF195 at 190.741 kb on - strand, within GFF195 at 190.819 kb on + strand at 190.820 kb on - strand at 190.897 kb on - strand at 191.041 kb on + strand at 191.042 kb on - strand at 191.113 kb on - strand at 191.122 kb on - strand at 191.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 187,478 + GFF192 0.78 -1.1 187,479 - GFF192 0.78 -0.0 187,479 - GFF192 0.78 +1.9 187,605 - +0.8 187,605 - +0.8 188,288 - -1.0 188,391 - -0.4 188,391 - +0.6 188,414 + -0.5 188,415 - +0.4 188,805 + GFF194 0.19 +4.6 188,996 - GFF194 0.29 -2.3 189,011 - GFF194 0.30 -0.5 189,011 - GFF194 0.30 -1.9 189,261 - GFF194 0.44 -1.0 189,317 + GFF194 0.47 -0.9 189,318 - GFF194 0.47 +1.5 189,997 + GFF194 0.83 -0.9 189,998 - GFF194 0.83 -1.3 190,055 + GFF194 0.86 -1.9 190,241 + -0.9 190,300 + -0.4 190,300 + +0.1 190,300 + -0.3 190,301 - -0.2 190,336 - +0.6 190,346 + -1.5 190,347 - -1.7 190,740 + GFF195 0.82 +0.3 190,741 - GFF195 0.82 -0.5 190,819 + +1.7 190,820 - +1.1 190,897 - -1.2 191,041 + -0.9 191,042 - -1.3 191,113 - -2.2 191,122 - +0.8 191,145 + -0.3
Or see this region's nucleotide sequence