Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1914 and GFF1915 are separated by 51 nucleotides GFF1915 and GFF1916 are separated by 10 nucleotides GFF1916 and GFF1917 overlap by 4 nucleotides GFF1917 and GFF1918 are separated by 182 nucleotides
GFF1914 - Cell division inhibitor, at 107,576 to 108,469
GFF1914
GFF1915 - Histidine ABC transporter, ATP-binding protein HisP (TC 3.A.1.3.1), at 108,521 to 109,297
GFF1915
GFF1916 - Histidine ABC transporter, permease protein HisM (TC 3.A.1.3.1), at 109,308 to 110,015
GFF1916
GFF1917 - Histidine ABC transporter, permease protein HisQ (TC 3.A.1.3.1), at 110,012 to 110,698
GFF1917
GFF1918 - Histidine ABC transporter, histidine-binding periplasmic protein precursor HisJ (TC 3.A.1.3.1), at 110,881 to 111,663
GFF1918
Position (kb) 109
110
111 Strain fitness (log2 ratio) -1
0
1
2 at 108.892 kb on - strand, within GFF1915 at 109.034 kb on + strand, within GFF1915 at 109.035 kb on - strand, within GFF1915 at 109.142 kb on - strand, within GFF1915 at 109.192 kb on + strand, within GFF1915 at 109.278 kb on - strand at 109.372 kb on + strand at 109.383 kb on + strand, within GFF1916 at 109.383 kb on + strand, within GFF1916 at 109.388 kb on - strand, within GFF1916 at 109.388 kb on - strand, within GFF1916 at 109.440 kb on - strand, within GFF1916 at 109.548 kb on + strand, within GFF1916 at 109.565 kb on + strand, within GFF1916 at 109.583 kb on + strand, within GFF1916 at 109.698 kb on + strand, within GFF1916 at 109.767 kb on - strand, within GFF1916 at 109.769 kb on - strand, within GFF1916 at 109.781 kb on - strand, within GFF1916 at 109.792 kb on - strand, within GFF1916 at 109.798 kb on - strand, within GFF1916 at 110.057 kb on + strand at 110.087 kb on - strand, within GFF1917 at 110.101 kb on + strand, within GFF1917 at 110.102 kb on - strand, within GFF1917 at 110.102 kb on - strand, within GFF1917 at 110.102 kb on - strand, within GFF1917 at 110.253 kb on + strand, within GFF1917 at 110.275 kb on + strand, within GFF1917 at 110.408 kb on - strand, within GFF1917 at 110.410 kb on + strand, within GFF1917 at 110.508 kb on - strand, within GFF1917 at 110.545 kb on - strand, within GFF1917 at 110.676 kb on - strand at 110.727 kb on + strand at 110.805 kb on + strand at 110.883 kb on - strand at 110.935 kb on - strand at 111.010 kb on - strand, within GFF1918
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 108,892 - GFF1915 0.48 +0.3 109,034 + GFF1915 0.66 +0.1 109,035 - GFF1915 0.66 +2.3 109,142 - GFF1915 0.80 +0.6 109,192 + GFF1915 0.86 +1.3 109,278 - -1.0 109,372 + -0.3 109,383 + GFF1916 0.11 -0.4 109,383 + GFF1916 0.11 +0.1 109,388 - GFF1916 0.11 -0.6 109,388 - GFF1916 0.11 +0.5 109,440 - GFF1916 0.19 -0.0 109,548 + GFF1916 0.34 -0.5 109,565 + GFF1916 0.36 +0.1 109,583 + GFF1916 0.39 -0.3 109,698 + GFF1916 0.55 +1.4 109,767 - GFF1916 0.65 +0.5 109,769 - GFF1916 0.65 +1.0 109,781 - GFF1916 0.67 +0.5 109,792 - GFF1916 0.68 +1.3 109,798 - GFF1916 0.69 +0.9 110,057 + +0.7 110,087 - GFF1917 0.11 +0.7 110,101 + GFF1917 0.13 +0.5 110,102 - GFF1917 0.13 +0.0 110,102 - GFF1917 0.13 -0.7 110,102 - GFF1917 0.13 -0.2 110,253 + GFF1917 0.35 +0.3 110,275 + GFF1917 0.38 +0.5 110,408 - GFF1917 0.58 -0.1 110,410 + GFF1917 0.58 +0.6 110,508 - GFF1917 0.72 -0.7 110,545 - GFF1917 0.78 -0.2 110,676 - +1.3 110,727 + +0.3 110,805 + +0.4 110,883 - +0.4 110,935 - -0.1 111,010 - GFF1918 0.16 +0.6
Or see this region's nucleotide sequence