Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1854 and GFF1855 are separated by 114 nucleotides GFF1855 and GFF1856 are separated by 146 nucleotides GFF1856 and GFF1857 are separated by 44 nucleotides GFF1857 and GFF1858 are separated by 280 nucleotides
GFF1854 - Molybdopterin binding motif, CinA N-terminal domain / C-terminal domain of CinA type E, at 46,858 to 48,054
GFF1854
GFF1855 - Uncharacterized protein YfaZ precursor, at 48,169 to 48,843
GFF1855
GFF1856 - Pyrimidine deoxynucleoside triphosphate (dYTP) pyrophosphohydrolase YfoO, at 48,990 to 49,415
GFF1856
GFF1857 - Polymyxin resistance protein PmrG; Ais protein, at 49,460 to 50,065
GFF1857
GFF1858 - UDP-4-amino-4-deoxy-L-arabinose--oxoglutarate aminotransferase (EC 2.6.1.-), at 50,346 to 51,503
GFF1858
Position (kb) 48
49
50 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 48.049 kb on + strand at 48.050 kb on - strand at 48.050 kb on - strand at 48.071 kb on + strand at 48.072 kb on - strand at 48.086 kb on - strand at 48.086 kb on - strand at 48.195 kb on - strand at 48.195 kb on - strand at 48.195 kb on - strand at 48.320 kb on + strand, within GFF1855 at 48.456 kb on + strand, within GFF1855 at 48.458 kb on + strand, within GFF1855 at 48.459 kb on - strand, within GFF1855 at 48.462 kb on + strand, within GFF1855 at 48.816 kb on + strand at 49.224 kb on - strand, within GFF1856 at 49.231 kb on - strand, within GFF1856 at 49.266 kb on + strand, within GFF1856 at 49.302 kb on - strand, within GFF1856 at 49.362 kb on + strand, within GFF1856 at 49.411 kb on + strand at 49.412 kb on - strand at 49.742 kb on - strand, within GFF1857 at 50.101 kb on + strand at 50.102 kb on - strand at 50.108 kb on - strand at 50.114 kb on - strand at 50.175 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 48,049 + +1.2 48,050 - -0.1 48,050 - -2.4 48,071 + -3.1 48,072 - -1.1 48,086 - +1.4 48,086 - +0.6 48,195 - -0.1 48,195 - +0.3 48,195 - +0.9 48,320 + GFF1855 0.22 -0.2 48,456 + GFF1855 0.43 -0.4 48,458 + GFF1855 0.43 +0.7 48,459 - GFF1855 0.43 -1.0 48,462 + GFF1855 0.43 -0.0 48,816 + -2.8 49,224 - GFF1856 0.55 +0.6 49,231 - GFF1856 0.57 +1.0 49,266 + GFF1856 0.65 +0.3 49,302 - GFF1856 0.73 +0.4 49,362 + GFF1856 0.87 +0.7 49,411 + +0.4 49,412 - +0.8 49,742 - GFF1857 0.47 -1.4 50,101 + +0.7 50,102 - +1.1 50,108 - +0.8 50,114 - +1.1 50,175 + +0.8
Or see this region's nucleotide sequence