Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1740 and GFF1741 are separated by 44 nucleotides GFF1741 and GFF1742 overlap by 1 nucleotides GFF1742 and GFF1743 are separated by 157 nucleotides
GFF1740 - Xanthine/uracil/thiamine/ascorbate permease family protein, at 40,768 to 42,105
GFF1740
GFF1741 - FIG00553873: hypothetical protein, at 42,150 to 42,734
GFF1741
GFF1742 - FIG004212: hypothetical protein, at 42,734 to 43,489
GFF1742
GFF1743 - Putative LysR-family transcriptional regulator YidZ, at 43,647 to 44,606
GFF1743
Position (kb) 42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 41.151 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.439 kb on - strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.444 kb on - strand, within GFF1740 at 41.452 kb on - strand, within GFF1740 at 41.661 kb on + strand, within GFF1740 at 41.661 kb on + strand, within GFF1740 at 41.662 kb on - strand, within GFF1740 at 41.662 kb on - strand, within GFF1740 at 41.713 kb on + strand, within GFF1740 at 41.715 kb on + strand, within GFF1740 at 41.796 kb on + strand, within GFF1740 at 41.797 kb on - strand, within GFF1740 at 41.833 kb on + strand, within GFF1740 at 41.895 kb on - strand, within GFF1740 at 41.999 kb on - strand at 42.068 kb on + strand at 42.143 kb on - strand at 42.464 kb on - strand, within GFF1741 at 42.468 kb on - strand, within GFF1741 at 42.469 kb on + strand, within GFF1741 at 42.470 kb on - strand, within GFF1741 at 42.528 kb on + strand, within GFF1741 at 42.582 kb on - strand, within GFF1741 at 42.693 kb on - strand at 42.716 kb on + strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.736 kb on - strand at 42.754 kb on - strand at 42.908 kb on + strand, within GFF1742 at 43.035 kb on - strand, within GFF1742 at 43.103 kb on + strand, within GFF1742 at 43.176 kb on - strand, within GFF1742 at 43.239 kb on + strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.240 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.330 kb on - strand, within GFF1742 at 43.336 kb on + strand, within GFF1742 at 43.459 kb on + strand at 43.460 kb on - strand at 43.460 kb on - strand at 43.531 kb on - strand at 43.615 kb on + strand at 43.686 kb on + strand at 43.687 kb on - strand at 43.706 kb on + strand at 43.715 kb on + strand at 43.716 kb on - strand at 43.728 kb on + strand at 43.731 kb on + strand at 43.731 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 41,151 + GFF1740 0.29 -1.1 41,438 + GFF1740 0.50 +0.4 41,438 + GFF1740 0.50 +0.5 41,438 + GFF1740 0.50 +1.0 41,439 - GFF1740 0.50 -0.1 41,443 + GFF1740 0.50 +1.7 41,443 + GFF1740 0.50 +0.1 41,444 - GFF1740 0.51 -2.3 41,452 - GFF1740 0.51 +1.8 41,661 + GFF1740 0.67 -0.6 41,661 + GFF1740 0.67 -0.9 41,662 - GFF1740 0.67 -1.1 41,662 - GFF1740 0.67 +1.8 41,713 + GFF1740 0.71 -0.6 41,715 + GFF1740 0.71 +0.6 41,796 + GFF1740 0.77 +0.2 41,797 - GFF1740 0.77 -2.3 41,833 + GFF1740 0.80 +0.7 41,895 - GFF1740 0.84 +0.2 41,999 - -0.2 42,068 + -3.4 42,143 - -0.3 42,464 - GFF1741 0.54 -3.3 42,468 - GFF1741 0.54 -1.2 42,469 + GFF1741 0.55 +1.8 42,470 - GFF1741 0.55 -1.3 42,528 + GFF1741 0.65 +1.1 42,582 - GFF1741 0.74 -0.8 42,693 - +1.8 42,716 + -0.4 42,717 - -1.8 42,717 - +0.6 42,717 - +0.9 42,736 - -1.9 42,754 - -0.3 42,908 + GFF1742 0.23 +1.2 43,035 - GFF1742 0.40 +0.5 43,103 + GFF1742 0.49 -2.3 43,176 - GFF1742 0.58 +0.9 43,239 + GFF1742 0.67 +0.4 43,240 - GFF1742 0.67 -3.4 43,240 - GFF1742 0.67 +0.8 43,330 - GFF1742 0.79 -0.5 43,330 - GFF1742 0.79 -2.1 43,336 + GFF1742 0.80 +0.9 43,459 + -0.5 43,460 - -0.6 43,460 - -0.7 43,531 - +0.5 43,615 + -0.3 43,686 + +1.8 43,687 - -1.2 43,706 + -1.6 43,715 + +1.1 43,716 - -0.3 43,728 + +1.7 43,731 + -0.3 43,731 + +0.7
Or see this region's nucleotide sequence