Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1681 and GFF1682 are separated by 45 nucleotides GFF1682 and GFF1683 are separated by 169 nucleotides GFF1683 and GFF1684 are separated by 74 nucleotides GFF1684 and GFF1685 are separated by 237 nucleotides
GFF1681 - Ferritin-like protein 2, at 65,812 to 66,309
GFF1681
GFF1682 - FIG00638619: hypothetical protein, at 66,355 to 66,594
GFF1682
GFF1683 - Tyrosine-specific transport protein, at 66,764 to 68,032
GFF1683
GFF1684 - FIG00637942: hypothetical protein, at 68,107 to 68,772
GFF1684
GFF1685 - FIG01045540: hypothetical protein, at 69,010 to 69,336
GFF1685
Position (kb) 66
67
68
69 Strain fitness (log2 ratio) -1
0
1
2 at 65.908 kb on + strand, within GFF1681 at 65.970 kb on + strand, within GFF1681 at 66.307 kb on + strand at 66.635 kb on + strand at 66.763 kb on - strand at 66.813 kb on + strand at 66.844 kb on + strand at 66.965 kb on + strand, within GFF1683 at 66.966 kb on - strand, within GFF1683 at 66.967 kb on + strand, within GFF1683 at 66.978 kb on + strand, within GFF1683 at 67.094 kb on + strand, within GFF1683 at 67.094 kb on + strand, within GFF1683 at 67.095 kb on - strand, within GFF1683 at 67.150 kb on + strand, within GFF1683 at 67.506 kb on - strand, within GFF1683 at 67.771 kb on + strand, within GFF1683 at 68.200 kb on + strand, within GFF1684 at 68.495 kb on + strand, within GFF1684 at 68.588 kb on + strand, within GFF1684 at 68.712 kb on - strand at 68.804 kb on + strand at 68.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 65,908 + GFF1681 0.19 +0.2 65,970 + GFF1681 0.32 +1.3 66,307 + +0.9 66,635 + +0.9 66,763 - +0.1 66,813 + +0.0 66,844 + -0.4 66,965 + GFF1683 0.16 -0.8 66,966 - GFF1683 0.16 +2.2 66,967 + GFF1683 0.16 +0.4 66,978 + GFF1683 0.17 -0.4 67,094 + GFF1683 0.26 +1.0 67,094 + GFF1683 0.26 -0.7 67,095 - GFF1683 0.26 +1.7 67,150 + GFF1683 0.30 -0.5 67,506 - GFF1683 0.58 +0.8 67,771 + GFF1683 0.79 +1.9 68,200 + GFF1684 0.14 -1.0 68,495 + GFF1684 0.58 +0.3 68,588 + GFF1684 0.72 +0.4 68,712 - -1.7 68,804 + +0.0 68,993 - +1.4
Or see this region's nucleotide sequence