Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1616 and GFF1617 are separated by 59 nucleotides GFF1617 and GFF1618 are separated by 373 nucleotides GFF1618 and GFF1619 are separated by 16 nucleotides
GFF1616 - FIG01047716: hypothetical protein, at 2,776 to 3,429
GFF1616
GFF1617 - Mobile element protein, at 3,489 to 4,343
GFF1617
GFF1618 - FIG00923183: possible membrane protein YebY, at 4,717 to 5,070
GFF1618
GFF1619 - Copper resistance protein D, at 5,087 to 5,962
GFF1619
Position (kb) 3
4
5 Strain fitness (log2 ratio) -2
-1
0
1
2 at 2.517 kb on - strand at 2.546 kb on - strand at 2.682 kb on - strand at 2.773 kb on + strand at 2.773 kb on + strand at 2.774 kb on - strand at 2.850 kb on + strand, within GFF1616 at 3.087 kb on + strand, within GFF1616 at 3.166 kb on + strand, within GFF1616 at 3.272 kb on + strand, within GFF1616 at 3.273 kb on - strand, within GFF1616 at 3.521 kb on - strand at 3.549 kb on - strand at 3.783 kb on - strand, within GFF1617 at 3.914 kb on - strand, within GFF1617 at 3.914 kb on - strand, within GFF1617 at 4.057 kb on + strand, within GFF1617 at 4.184 kb on + strand, within GFF1617 at 4.850 kb on - strand, within GFF1618 at 4.893 kb on + strand, within GFF1618 at 4.893 kb on + strand, within GFF1618 at 4.910 kb on + strand, within GFF1618 at 4.910 kb on + strand, within GFF1618 at 4.911 kb on - strand, within GFF1618 at 4.994 kb on - strand, within GFF1618 at 4.994 kb on - strand, within GFF1618 at 5.132 kb on + strand at 5.132 kb on + strand at 5.206 kb on + strand, within GFF1619 at 5.207 kb on - strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.251 kb on + strand, within GFF1619 at 5.252 kb on - strand, within GFF1619 at 5.252 kb on - strand, within GFF1619 at 5.293 kb on - strand, within GFF1619 at 5.331 kb on - strand, within GFF1619
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 2,517 - +0.9 2,546 - +0.2 2,682 - +1.4 2,773 + -0.4 2,773 + +2.0 2,774 - -0.0 2,850 + GFF1616 0.11 +0.3 3,087 + GFF1616 0.48 +0.2 3,166 + GFF1616 0.60 +1.4 3,272 + GFF1616 0.76 -0.2 3,273 - GFF1616 0.76 +0.3 3,521 - +0.5 3,549 - +0.6 3,783 - GFF1617 0.34 +0.9 3,914 - GFF1617 0.50 +0.8 3,914 - GFF1617 0.50 +1.0 4,057 + GFF1617 0.66 +0.6 4,184 + GFF1617 0.81 -1.4 4,850 - GFF1618 0.38 +0.8 4,893 + GFF1618 0.50 -0.6 4,893 + GFF1618 0.50 +0.4 4,910 + GFF1618 0.55 -2.3 4,910 + GFF1618 0.55 -0.1 4,911 - GFF1618 0.55 -0.1 4,994 - GFF1618 0.78 +1.0 4,994 - GFF1618 0.78 -1.3 5,132 + +0.9 5,132 + +1.5 5,206 + GFF1619 0.14 +0.9 5,207 - GFF1619 0.14 +0.5 5,251 + GFF1619 0.19 -0.7 5,251 + GFF1619 0.19 +0.6 5,251 + GFF1619 0.19 +0.4 5,252 - GFF1619 0.19 -0.3 5,252 - GFF1619 0.19 +1.2 5,293 - GFF1619 0.24 +0.3 5,331 - GFF1619 0.28 -0.4
Or see this region's nucleotide sequence