Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1509 and GFF1510 are separated by 8 nucleotides GFF1510 and GFF1511 are separated by 216 nucleotides GFF1511 and GFF1512 overlap by 7 nucleotides
GFF1509 - FIG139928: Putative protease, at 16,403 to 17,281
GFF1509
GFF1510 - FIG139552: Putative protease, at 17,290 to 18,285
GFF1510
GFF1511 - FIG138517: Putative lipid carrier protein, at 18,502 to 19,026
GFF1511
GFF1512 - FIG002208: Acetyltransferase (EC 2.3.1.-), at 19,020 to 19,523
GFF1512
Position (kb)
17
18
19 Strain fitness (log2 ratio)
-2
-1
0
1 at 16.303 kb on - strand at 16.370 kb on + strand at 16.385 kb on - strand at 16.385 kb on - strand at 16.599 kb on + strand, within GFF1509 at 16.599 kb on + strand, within GFF1509 at 16.690 kb on + strand, within GFF1509 at 16.690 kb on + strand, within GFF1509 at 16.873 kb on + strand, within GFF1509 at 16.884 kb on - strand, within GFF1509 at 17.032 kb on - strand, within GFF1509 at 17.048 kb on + strand, within GFF1509 at 17.253 kb on + strand at 17.253 kb on + strand at 17.275 kb on - strand at 17.275 kb on - strand at 17.302 kb on - strand at 17.309 kb on + strand at 17.579 kb on + strand, within GFF1510 at 17.580 kb on - strand, within GFF1510 at 17.775 kb on + strand, within GFF1510 at 17.831 kb on - strand, within GFF1510 at 17.869 kb on + strand, within GFF1510 at 18.344 kb on + strand at 18.344 kb on + strand at 18.387 kb on + strand at 18.527 kb on + strand at 18.527 kb on + strand at 18.880 kb on + strand, within GFF1511 at 18.880 kb on + strand, within GFF1511 at 18.881 kb on - strand, within GFF1511 at 19.024 kb on + strand at 19.024 kb on + strand at 19.058 kb on + strand at 19.225 kb on + strand, within GFF1512
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 16,303 - -1.5 16,370 + -0.1 16,385 - +0.4 16,385 - -1.1 16,599 + GFF1509 0.22 -2.2 16,599 + GFF1509 0.22 -0.1 16,690 + GFF1509 0.33 -0.1 16,690 + GFF1509 0.33 +1.7 16,873 + GFF1509 0.53 -0.7 16,884 - GFF1509 0.55 -1.9 17,032 - GFF1509 0.72 -0.9 17,048 + GFF1509 0.73 -2.2 17,253 + -1.3 17,253 + -0.9 17,275 - +0.8 17,275 - -0.8 17,302 - -1.4 17,309 + +0.4 17,579 + GFF1510 0.29 +0.4 17,580 - GFF1510 0.29 +0.5 17,775 + GFF1510 0.49 +0.2 17,831 - GFF1510 0.54 +0.1 17,869 + GFF1510 0.58 -1.5 18,344 + -1.4 18,344 + -0.3 18,387 + -1.6 18,527 + +0.6 18,527 + -0.6 18,880 + GFF1511 0.72 +0.1 18,880 + GFF1511 0.72 +0.2 18,881 - GFF1511 0.72 +1.1 19,024 + -1.8 19,024 + +0.1 19,058 + -1.5 19,225 + GFF1512 0.41 +0.2
Or see this region's nucleotide sequence