Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1508 and GFF1509 are separated by 177 nucleotides GFF1509 and GFF1510 are separated by 8 nucleotides
GFF1508 - Luciferase-like monooxygenase (EC 1.14.-.-), at 15,218 to 16,225
GFF1508
GFF1509 - FIG139928: Putative protease, at 16,403 to 17,281
GFF1509
GFF1510 - FIG139552: Putative protease, at 17,290 to 18,285
GFF1510
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-2
-1
0
1 at 15.436 kb on + strand, within GFF1508 at 15.556 kb on + strand, within GFF1508 at 15.700 kb on + strand, within GFF1508 at 15.702 kb on + strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.703 kb on - strand, within GFF1508 at 15.902 kb on + strand, within GFF1508 at 15.903 kb on - strand, within GFF1508 at 16.004 kb on + strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.005 kb on - strand, within GFF1508 at 16.030 kb on - strand, within GFF1508 at 16.211 kb on - strand at 16.211 kb on - strand at 16.256 kb on + strand at 16.303 kb on - strand at 16.370 kb on + strand at 16.385 kb on - strand at 16.385 kb on - strand at 16.599 kb on + strand, within GFF1509 at 16.599 kb on + strand, within GFF1509 at 16.690 kb on + strand, within GFF1509 at 16.690 kb on + strand, within GFF1509 at 16.873 kb on + strand, within GFF1509 at 16.884 kb on - strand, within GFF1509 at 17.032 kb on - strand, within GFF1509 at 17.048 kb on + strand, within GFF1509 at 17.253 kb on + strand at 17.253 kb on + strand at 17.275 kb on - strand at 17.275 kb on - strand at 17.302 kb on - strand at 17.309 kb on + strand at 17.579 kb on + strand, within GFF1510 at 17.580 kb on - strand, within GFF1510 at 17.775 kb on + strand, within GFF1510 at 17.831 kb on - strand, within GFF1510 at 17.869 kb on + strand, within GFF1510
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 15,436 + GFF1508 0.22 -0.2 15,556 + GFF1508 0.34 +0.7 15,700 + GFF1508 0.48 +1.2 15,702 + GFF1508 0.48 +0.5 15,703 - GFF1508 0.48 -0.3 15,703 - GFF1508 0.48 -0.3 15,703 - GFF1508 0.48 -0.2 15,703 - GFF1508 0.48 +0.5 15,902 + GFF1508 0.68 -1.7 15,903 - GFF1508 0.68 -0.5 16,004 + GFF1508 0.78 -1.5 16,005 - GFF1508 0.78 -0.3 16,005 - GFF1508 0.78 -1.5 16,005 - GFF1508 0.78 -1.7 16,005 - GFF1508 0.78 -0.1 16,005 - GFF1508 0.78 -1.6 16,030 - GFF1508 0.81 -0.7 16,211 - +0.2 16,211 - -1.9 16,256 + -0.3 16,303 - -1.5 16,370 + -0.1 16,385 - +0.4 16,385 - -1.1 16,599 + GFF1509 0.22 -2.2 16,599 + GFF1509 0.22 -0.1 16,690 + GFF1509 0.33 -0.1 16,690 + GFF1509 0.33 +1.7 16,873 + GFF1509 0.53 -0.7 16,884 - GFF1509 0.55 -1.9 17,032 - GFF1509 0.72 -0.9 17,048 + GFF1509 0.73 -2.2 17,253 + -1.3 17,253 + -0.9 17,275 - +0.8 17,275 - -0.8 17,302 - -1.4 17,309 + +0.4 17,579 + GFF1510 0.29 +0.4 17,580 - GFF1510 0.29 +0.5 17,775 + GFF1510 0.49 +0.2 17,831 - GFF1510 0.54 +0.1 17,869 + GFF1510 0.58 -1.5
Or see this region's nucleotide sequence