Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1467 and GFF1468 are separated by 166 nucleotides GFF1468 and GFF1469 are separated by 0 nucleotides GFF1469 and GFF1470 overlap by 4 nucleotides
GFF1467 - L-Proline/Glycine betaine transporter ProP, at 131,101 to 132,603
GFF1467
GFF1468 - Sensor protein basS/pmrB (EC 2.7.3.-), at 132,770 to 133,849
GFF1468
GFF1469 - Transcriptional regulatory protein basR/pmrA, at 133,850 to 134,518
GFF1469
GFF1470 - Phosphoethanolamine transferase EptA specific for the 1 phosphate group of core-lipid A, at 134,515 to 136,158
GFF1470
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 131.926 kb on + strand, within GFF1467 at 131.926 kb on + strand, within GFF1467 at 131.927 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.942 kb on - strand, within GFF1467 at 131.953 kb on + strand, within GFF1467 at 131.954 kb on - strand, within GFF1467 at 131.964 kb on + strand, within GFF1467 at 131.964 kb on + strand, within GFF1467 at 131.965 kb on - strand, within GFF1467 at 131.974 kb on + strand, within GFF1467 at 132.145 kb on - strand, within GFF1467 at 132.205 kb on - strand, within GFF1467 at 132.406 kb on - strand, within GFF1467 at 132.507 kb on + strand at 132.508 kb on - strand at 132.641 kb on + strand at 132.683 kb on - strand at 132.711 kb on + strand at 132.711 kb on + strand at 132.739 kb on + strand at 132.740 kb on - strand at 132.757 kb on + strand at 132.761 kb on - strand at 132.771 kb on + strand at 132.848 kb on + strand at 132.848 kb on + strand at 132.930 kb on - strand, within GFF1468 at 132.930 kb on - strand, within GFF1468 at 133.038 kb on + strand, within GFF1468 at 133.038 kb on + strand, within GFF1468 at 133.039 kb on - strand, within GFF1468 at 133.083 kb on - strand, within GFF1468 at 133.083 kb on - strand, within GFF1468 at 133.176 kb on + strand, within GFF1468 at 133.176 kb on + strand, within GFF1468 at 133.177 kb on - strand, within GFF1468 at 133.219 kb on - strand, within GFF1468 at 133.340 kb on + strand, within GFF1468 at 133.379 kb on + strand, within GFF1468 at 133.517 kb on - strand, within GFF1468 at 133.615 kb on + strand, within GFF1468 at 133.616 kb on - strand, within GFF1468 at 133.669 kb on + strand, within GFF1468 at 133.754 kb on - strand at 133.754 kb on - strand at 133.780 kb on + strand at 133.851 kb on + strand at 133.878 kb on + strand at 133.933 kb on + strand, within GFF1469 at 133.933 kb on + strand, within GFF1469 at 133.937 kb on + strand, within GFF1469 at 133.937 kb on + strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.938 kb on - strand, within GFF1469 at 133.939 kb on + strand, within GFF1469 at 133.939 kb on + strand, within GFF1469 at 133.940 kb on - strand, within GFF1469 at 133.984 kb on - strand, within GFF1469 at 133.984 kb on - strand, within GFF1469 at 133.993 kb on + strand, within GFF1469 at 133.994 kb on - strand, within GFF1469 at 133.995 kb on + strand, within GFF1469 at 134.056 kb on - strand, within GFF1469 at 134.111 kb on + strand, within GFF1469 at 134.112 kb on - strand, within GFF1469 at 134.114 kb on - strand, within GFF1469 at 134.114 kb on - strand, within GFF1469 at 134.162 kb on - strand, within GFF1469 at 134.307 kb on + strand, within GFF1469 at 134.404 kb on + strand, within GFF1469 at 134.469 kb on - strand at 134.483 kb on + strand at 134.484 kb on - strand at 134.567 kb on + strand at 134.568 kb on - strand at 134.579 kb on - strand at 134.673 kb on - strand at 134.805 kb on - strand, within GFF1470
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 131,926 + GFF1467 0.55 +0.4 131,926 + GFF1467 0.55 -2.5 131,927 - GFF1467 0.55 +0.2 131,942 - GFF1467 0.56 +0.7 131,942 - GFF1467 0.56 -0.6 131,942 - GFF1467 0.56 -2.7 131,942 - GFF1467 0.56 -0.7 131,953 + GFF1467 0.57 +0.2 131,954 - GFF1467 0.57 +0.4 131,964 + GFF1467 0.57 -0.8 131,964 + GFF1467 0.57 +2.8 131,965 - GFF1467 0.57 +0.2 131,974 + GFF1467 0.58 +1.9 132,145 - GFF1467 0.69 -0.6 132,205 - GFF1467 0.73 +1.3 132,406 - GFF1467 0.87 +0.9 132,507 + -1.0 132,508 - +1.3 132,641 + -0.1 132,683 - +1.6 132,711 + +0.1 132,711 + -1.0 132,739 + +3.2 132,740 - -0.3 132,757 + +0.9 132,761 - +0.3 132,771 + +0.7 132,848 + -0.0 132,848 + -1.3 132,930 - GFF1468 0.15 +1.8 132,930 - GFF1468 0.15 +2.4 133,038 + GFF1468 0.25 +0.2 133,038 + GFF1468 0.25 -2.0 133,039 - GFF1468 0.25 +1.0 133,083 - GFF1468 0.29 -0.4 133,083 - GFF1468 0.29 +2.7 133,176 + GFF1468 0.38 +0.3 133,176 + GFF1468 0.38 -2.3 133,177 - GFF1468 0.38 +0.1 133,219 - GFF1468 0.42 -0.6 133,340 + GFF1468 0.53 -1.0 133,379 + GFF1468 0.56 +1.1 133,517 - GFF1468 0.69 -0.2 133,615 + GFF1468 0.78 +3.6 133,616 - GFF1468 0.78 -0.5 133,669 + GFF1468 0.83 -1.0 133,754 - -1.6 133,754 - +0.6 133,780 + +1.2 133,851 + +1.2 133,878 + +0.7 133,933 + GFF1469 0.12 +0.5 133,933 + GFF1469 0.12 +0.5 133,937 + GFF1469 0.13 -0.1 133,937 + GFF1469 0.13 +0.7 133,938 - GFF1469 0.13 -1.9 133,938 - GFF1469 0.13 -2.8 133,938 - GFF1469 0.13 -0.1 133,939 + GFF1469 0.13 +0.0 133,939 + GFF1469 0.13 -1.2 133,940 - GFF1469 0.13 +0.3 133,984 - GFF1469 0.20 -1.4 133,984 - GFF1469 0.20 -1.7 133,993 + GFF1469 0.21 -1.0 133,994 - GFF1469 0.22 -0.6 133,995 + GFF1469 0.22 -2.1 134,056 - GFF1469 0.31 -1.6 134,111 + GFF1469 0.39 -1.2 134,112 - GFF1469 0.39 +3.0 134,114 - GFF1469 0.39 -1.2 134,114 - GFF1469 0.39 -1.0 134,162 - GFF1469 0.47 -1.6 134,307 + GFF1469 0.68 -1.3 134,404 + GFF1469 0.83 -3.4 134,469 - +0.3 134,483 + +1.1 134,484 - -0.4 134,567 + +0.5 134,568 - -1.0 134,579 - -1.6 134,673 - -1.4 134,805 - GFF1470 0.18 -0.0
Or see this region's nucleotide sequence