Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1464 and GFF1465 are separated by 151 nucleotides GFF1465 and GFF1466 are separated by 119 nucleotides GFF1466 and GFF1467 are separated by 467 nucleotides
GFF1464 - PhnO protein, at 129,149 to 129,583
GFF1464
GFF1465 - PhnB protein; putative DNA binding 3-demethylubiquinone-9 3-methyltransferase domain protein, at 129,735 to 130,178
GFF1465
GFF1466 - Alkylphosphonate utilization operon protein PhnA, at 130,298 to 130,633
GFF1466
GFF1467 - L-Proline/Glycine betaine transporter ProP, at 131,101 to 132,603
GFF1467
Position (kb) 130
131 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 129.388 kb on + strand, within GFF1464 at 129.389 kb on - strand, within GFF1464 at 129.512 kb on + strand, within GFF1464 at 129.523 kb on + strand, within GFF1464 at 129.640 kb on + strand at 129.654 kb on - strand at 129.654 kb on - strand at 129.659 kb on - strand at 129.659 kb on - strand at 129.736 kb on + strand at 129.764 kb on + strand at 130.089 kb on - strand, within GFF1465 at 130.089 kb on - strand, within GFF1465 at 130.090 kb on + strand, within GFF1465 at 130.090 kb on + strand, within GFF1465 at 130.091 kb on - strand, within GFF1465 at 130.121 kb on - strand, within GFF1465 at 130.123 kb on + strand, within GFF1465 at 130.185 kb on + strand at 130.208 kb on + strand at 130.367 kb on - strand, within GFF1466 at 130.430 kb on - strand, within GFF1466 at 130.590 kb on + strand, within GFF1466 at 130.591 kb on - strand, within GFF1466 at 130.825 kb on + strand at 130.825 kb on + strand at 130.840 kb on + strand at 130.841 kb on - strand at 130.863 kb on + strand at 130.863 kb on + strand at 130.863 kb on + strand at 130.864 kb on - strand at 130.864 kb on - strand at 130.864 kb on - strand at 130.865 kb on + strand at 130.865 kb on + strand at 130.865 kb on + strand at 130.866 kb on - strand at 130.866 kb on - strand at 130.916 kb on + strand at 130.950 kb on + strand at 130.950 kb on + strand at 130.950 kb on + strand at 130.972 kb on + strand at 130.972 kb on + strand at 130.972 kb on + strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.973 kb on - strand at 130.993 kb on + strand at 131.003 kb on - strand at 131.047 kb on + strand at 131.065 kb on - strand at 131.066 kb on + strand at 131.066 kb on + strand at 131.067 kb on - strand at 131.069 kb on - strand at 131.131 kb on - strand at 131.163 kb on + strand at 131.257 kb on - strand, within GFF1467 at 131.377 kb on + strand, within GFF1467 at 131.378 kb on - strand, within GFF1467 at 131.416 kb on - strand, within GFF1467
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 129,388 + GFF1464 0.55 +0.4 129,389 - GFF1464 0.55 +1.2 129,512 + GFF1464 0.83 +1.0 129,523 + GFF1464 0.86 -2.4 129,640 + +1.1 129,654 - +0.1 129,654 - +1.1 129,659 - -0.6 129,659 - -2.4 129,736 + -1.7 129,764 + +0.2 130,089 - GFF1465 0.80 -1.1 130,089 - GFF1465 0.80 -0.3 130,090 + GFF1465 0.80 -0.3 130,090 + GFF1465 0.80 -1.0 130,091 - GFF1465 0.80 -0.8 130,121 - GFF1465 0.87 -0.8 130,123 + GFF1465 0.87 +0.3 130,185 + -1.2 130,208 + -0.1 130,367 - GFF1466 0.21 +3.7 130,430 - GFF1466 0.39 -0.1 130,590 + GFF1466 0.87 -0.2 130,591 - GFF1466 0.87 -1.4 130,825 + +0.2 130,825 + +0.5 130,840 + -0.1 130,841 - -0.2 130,863 + +0.6 130,863 + -2.2 130,863 + +0.5 130,864 - -1.4 130,864 - -1.7 130,864 - -0.0 130,865 + +0.1 130,865 + -2.5 130,865 + -1.2 130,866 - -0.4 130,866 - +0.9 130,916 + +0.9 130,950 + +0.1 130,950 + -2.7 130,950 + -1.1 130,972 + -0.1 130,972 + -0.5 130,972 + +2.1 130,973 - +0.5 130,973 - +0.3 130,973 - +0.9 130,973 - -0.8 130,973 - -0.3 130,973 - +1.4 130,993 + -1.0 131,003 - +0.8 131,047 + +0.6 131,065 - +1.2 131,066 + -0.3 131,066 + -1.1 131,067 - -1.0 131,069 - -3.4 131,131 - -0.2 131,163 + +0.4 131,257 - GFF1467 0.10 +0.2 131,377 + GFF1467 0.18 -0.3 131,378 - GFF1467 0.18 -1.2 131,416 - GFF1467 0.21 +0.5
Or see this region's nucleotide sequence