Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1447 and GFF1448 are separated by 103 nucleotides GFF1448 and GFF1449 overlap by 8 nucleotides GFF1449 and GFF1450 are separated by 38 nucleotides
GFF1447 - LysR family regulatory protein CidR, at 109,517 to 110,404
GFF1447
GFF1448 - Holin-like protein CidA, at 110,508 to 110,918
GFF1448
GFF1449 - CidA-associated membrane protein CidB, at 110,911 to 111,600
GFF1449
GFF1450 - Acetate permease ActP (cation/acetate symporter), at 111,639 to 113,288
GFF1450
Position (kb)
110
111 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 109.582 kb on - strand at 109.766 kb on + strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.767 kb on - strand, within GFF1447 at 109.784 kb on + strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.785 kb on - strand, within GFF1447 at 109.825 kb on - strand, within GFF1447 at 109.828 kb on + strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 109.829 kb on - strand, within GFF1447 at 110.013 kb on - strand, within GFF1447 at 110.152 kb on + strand, within GFF1447 at 110.240 kb on + strand, within GFF1447 at 110.241 kb on - strand, within GFF1447 at 110.264 kb on + strand, within GFF1447 at 110.270 kb on - strand, within GFF1447 at 110.275 kb on + strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.276 kb on - strand, within GFF1447 at 110.330 kb on + strand at 110.382 kb on + strand at 110.438 kb on + strand at 110.470 kb on - strand at 110.470 kb on - strand at 110.531 kb on + strand at 110.591 kb on - strand, within GFF1448 at 110.663 kb on + strand, within GFF1448 at 110.663 kb on + strand, within GFF1448 at 110.664 kb on - strand, within GFF1448 at 110.699 kb on + strand, within GFF1448 at 110.782 kb on - strand, within GFF1448 at 110.842 kb on + strand, within GFF1448 at 110.842 kb on + strand, within GFF1448 at 110.849 kb on - strand, within GFF1448 at 110.890 kb on - strand at 110.890 kb on - strand at 110.934 kb on - strand at 110.984 kb on - strand, within GFF1449 at 111.017 kb on + strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.064 kb on - strand, within GFF1449 at 111.079 kb on + strand, within GFF1449 at 111.291 kb on - strand, within GFF1449 at 111.537 kb on - strand at 111.640 kb on + strand at 111.641 kb on - strand at 111.779 kb on + strand at 111.905 kb on + strand, within GFF1450
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 109,582 - -1.6 109,766 + GFF1447 0.28 -0.6 109,767 - GFF1447 0.28 +0.1 109,767 - GFF1447 0.28 +0.7 109,784 + GFF1447 0.30 -1.1 109,785 - GFF1447 0.30 -0.8 109,785 - GFF1447 0.30 -1.8 109,825 - GFF1447 0.35 -0.7 109,828 + GFF1447 0.35 -0.2 109,829 - GFF1447 0.35 -2.2 109,829 - GFF1447 0.35 +0.6 109,829 - GFF1447 0.35 +0.7 109,829 - GFF1447 0.35 +1.2 110,013 - GFF1447 0.56 +0.0 110,152 + GFF1447 0.72 -0.2 110,240 + GFF1447 0.81 +0.2 110,241 - GFF1447 0.82 +0.6 110,264 + GFF1447 0.84 -0.4 110,270 - GFF1447 0.85 -0.7 110,275 + GFF1447 0.85 +0.2 110,276 - GFF1447 0.85 -0.4 110,276 - GFF1447 0.85 +0.0 110,330 + -1.6 110,382 + -0.3 110,438 + -1.0 110,470 - -2.2 110,470 - -0.6 110,531 + +0.7 110,591 - GFF1448 0.20 -0.2 110,663 + GFF1448 0.38 +0.4 110,663 + GFF1448 0.38 -0.8 110,664 - GFF1448 0.38 -1.8 110,699 + GFF1448 0.46 -1.9 110,782 - GFF1448 0.67 -0.0 110,842 + GFF1448 0.81 -1.4 110,842 + GFF1448 0.81 +0.3 110,849 - GFF1448 0.83 -0.8 110,890 - +0.6 110,890 - +0.7 110,934 - +0.8 110,984 - GFF1449 0.11 +1.5 111,017 + GFF1449 0.15 -0.6 111,064 - GFF1449 0.22 +0.7 111,064 - GFF1449 0.22 +3.3 111,064 - GFF1449 0.22 +2.3 111,079 + GFF1449 0.24 +0.9 111,291 - GFF1449 0.55 +0.1 111,537 - -0.1 111,640 + -0.0 111,641 - +0.8 111,779 + -0.0 111,905 + GFF1450 0.16 +2.7
Or see this region's nucleotide sequence