Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1415 and GFF1416 are separated by 98 nucleotides GFF1416 and GFF1417 are separated by 246 nucleotides GFF1417 and GFF1418 are separated by 87 nucleotides
GFF1415 - UPF0337 protein yjbJ, at 61,620 to 61,832
GFF1415
GFF1416 - Zinc uptake regulation protein ZUR, at 61,931 to 62,446
GFF1416
GFF1417 - FIG01200701: possible membrane protein, at 62,693 to 64,003
GFF1417
GFF1418 - tRNA dihydrouridine synthase A (EC 1.-.-.-), at 64,091 to 65,089
GFF1418
Position (kb)
62
63
64
65 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 61.742 kb on - strand, within GFF1415 at 61.834 kb on - strand at 61.854 kb on + strand at 61.854 kb on + strand at 61.855 kb on - strand at 61.882 kb on + strand at 61.882 kb on + strand at 61.907 kb on - strand at 62.000 kb on + strand, within GFF1416 at 62.000 kb on + strand, within GFF1416 at 62.001 kb on - strand, within GFF1416 at 62.001 kb on - strand, within GFF1416 at 62.007 kb on - strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.044 kb on + strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.045 kb on - strand, within GFF1416 at 62.193 kb on + strand, within GFF1416 at 62.194 kb on - strand, within GFF1416 at 62.197 kb on + strand, within GFF1416 at 62.198 kb on - strand, within GFF1416 at 62.198 kb on - strand, within GFF1416 at 62.257 kb on - strand, within GFF1416 at 62.448 kb on + strand at 62.448 kb on + strand at 62.449 kb on - strand at 62.449 kb on - strand at 62.449 kb on - strand at 62.481 kb on + strand at 62.482 kb on - strand at 62.487 kb on - strand at 62.500 kb on + strand at 62.567 kb on + strand at 62.567 kb on + strand at 62.569 kb on + strand at 62.569 kb on + strand at 62.570 kb on - strand at 62.574 kb on - strand at 62.590 kb on - strand at 62.592 kb on - strand at 62.676 kb on - strand at 62.678 kb on + strand at 62.679 kb on - strand at 62.847 kb on + strand, within GFF1417 at 62.848 kb on - strand, within GFF1417 at 62.887 kb on + strand, within GFF1417 at 62.979 kb on - strand, within GFF1417 at 62.981 kb on + strand, within GFF1417 at 62.981 kb on + strand, within GFF1417 at 62.982 kb on - strand, within GFF1417 at 62.982 kb on - strand, within GFF1417 at 63.089 kb on + strand, within GFF1417 at 63.186 kb on - strand, within GFF1417 at 63.357 kb on + strand, within GFF1417 at 63.358 kb on - strand, within GFF1417 at 63.455 kb on - strand, within GFF1417 at 63.458 kb on + strand, within GFF1417 at 63.500 kb on - strand, within GFF1417 at 63.629 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.633 kb on - strand, within GFF1417 at 63.646 kb on + strand, within GFF1417 at 63.646 kb on + strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.677 kb on - strand, within GFF1417 at 63.681 kb on - strand, within GFF1417 at 63.775 kb on + strand, within GFF1417 at 63.797 kb on + strand, within GFF1417 at 63.797 kb on + strand, within GFF1417 at 63.940 kb on - strand at 63.989 kb on - strand at 63.989 kb on - strand at 63.989 kb on - strand at 64.049 kb on - strand at 64.127 kb on + strand at 64.147 kb on + strand at 64.253 kb on - strand, within GFF1418 at 64.274 kb on - strand, within GFF1418 at 64.365 kb on - strand, within GFF1418 at 64.506 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.541 kb on + strand, within GFF1418 at 64.542 kb on - strand, within GFF1418 at 64.542 kb on - strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.898 kb on + strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.899 kb on - strand, within GFF1418 at 64.907 kb on - strand, within GFF1418 at 64.934 kb on + strand, within GFF1418
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 61,742 - GFF1415 0.57 +0.0 61,834 - +1.8 61,854 + +1.8 61,854 + -1.2 61,855 - -0.2 61,882 + -0.6 61,882 + +0.5 61,907 - +0.2 62,000 + GFF1416 0.13 -1.0 62,000 + GFF1416 0.13 +1.1 62,001 - GFF1416 0.14 +0.6 62,001 - GFF1416 0.14 -1.7 62,007 - GFF1416 0.15 +1.3 62,044 + GFF1416 0.22 +0.4 62,044 + GFF1416 0.22 -3.6 62,044 + GFF1416 0.22 +0.3 62,045 - GFF1416 0.22 +1.4 62,045 - GFF1416 0.22 +1.2 62,045 - GFF1416 0.22 -0.3 62,193 + GFF1416 0.51 -0.1 62,194 - GFF1416 0.51 +0.8 62,197 + GFF1416 0.52 -2.9 62,198 - GFF1416 0.52 +0.1 62,198 - GFF1416 0.52 -2.1 62,257 - GFF1416 0.63 +0.5 62,448 + +0.8 62,448 + +0.2 62,449 - +0.2 62,449 - -0.5 62,449 - -0.4 62,481 + -1.6 62,482 - +1.0 62,487 - -0.6 62,500 + -3.7 62,567 + +0.1 62,567 + +0.7 62,569 + -0.3 62,569 + -0.3 62,570 - +2.2 62,574 - +0.5 62,590 - +0.2 62,592 - -0.3 62,676 - -1.7 62,678 + +0.9 62,679 - +0.4 62,847 + GFF1417 0.12 +0.9 62,848 - GFF1417 0.12 -1.0 62,887 + GFF1417 0.15 +1.0 62,979 - GFF1417 0.22 +0.3 62,981 + GFF1417 0.22 -0.7 62,981 + GFF1417 0.22 +0.2 62,982 - GFF1417 0.22 -0.9 62,982 - GFF1417 0.22 -3.7 63,089 + GFF1417 0.30 +0.1 63,186 - GFF1417 0.38 -1.7 63,357 + GFF1417 0.51 +0.9 63,358 - GFF1417 0.51 +2.0 63,455 - GFF1417 0.58 -2.9 63,458 + GFF1417 0.58 -0.4 63,500 - GFF1417 0.62 -2.3 63,629 - GFF1417 0.71 -0.6 63,633 - GFF1417 0.72 -0.9 63,633 - GFF1417 0.72 -1.2 63,633 - GFF1417 0.72 -0.4 63,633 - GFF1417 0.72 +1.7 63,646 + GFF1417 0.73 +0.4 63,646 + GFF1417 0.73 -2.3 63,677 - GFF1417 0.75 -0.6 63,677 - GFF1417 0.75 +0.5 63,677 - GFF1417 0.75 -0.7 63,681 - GFF1417 0.75 -0.1 63,775 + GFF1417 0.83 -0.8 63,797 + GFF1417 0.84 +0.8 63,797 + GFF1417 0.84 -0.1 63,940 - -0.5 63,989 - -0.6 63,989 - +0.4 63,989 - -2.1 64,049 - +0.2 64,127 + -0.4 64,147 + -0.7 64,253 - GFF1418 0.16 -0.2 64,274 - GFF1418 0.18 -3.4 64,365 - GFF1418 0.27 +2.2 64,506 + GFF1418 0.42 +0.3 64,541 + GFF1418 0.45 -0.8 64,541 + GFF1418 0.45 -3.0 64,541 + GFF1418 0.45 -0.0 64,541 + GFF1418 0.45 -0.2 64,542 - GFF1418 0.45 +0.1 64,542 - GFF1418 0.45 -0.0 64,898 + GFF1418 0.81 +0.8 64,898 + GFF1418 0.81 -0.2 64,898 + GFF1418 0.81 -0.2 64,899 - GFF1418 0.81 +0.8 64,899 - GFF1418 0.81 -0.6 64,907 - GFF1418 0.82 +0.9 64,934 + GFF1418 0.84 +0.9
Or see this region's nucleotide sequence