Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1373 and GFF1374 overlap by 10 nucleotides GFF1374 and GFF1375 are separated by 60 nucleotides GFF1375 and GFF1376 overlap by 20 nucleotides
GFF1373 - Phage baseplate, at 22,945 to 24,060
GFF1373
GFF1374 - putative bacteriophage baseplate protein, at 24,051 to 24,410
GFF1374
GFF1375 - hypothetical protein, at 24,471 to 24,593
GFF1375
GFF1376 - putative inner membrane protein, at 24,574 to 26,112
GFF1376
Position (kb) 24
25 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 23.065 kb on + strand, within GFF1373 at 23.185 kb on - strand, within GFF1373 at 23.236 kb on - strand, within GFF1373 at 23.442 kb on - strand, within GFF1373 at 23.488 kb on - strand, within GFF1373 at 23.488 kb on - strand, within GFF1373 at 23.488 kb on - strand, within GFF1373 at 23.488 kb on - strand, within GFF1373 at 23.558 kb on + strand, within GFF1373 at 23.644 kb on + strand, within GFF1373 at 23.645 kb on - strand, within GFF1373 at 23.990 kb on + strand at 24.036 kb on + strand at 24.061 kb on - strand at 24.313 kb on - strand, within GFF1374 at 24.346 kb on + strand, within GFF1374 at 24.445 kb on - strand at 24.445 kb on - strand at 24.465 kb on + strand at 24.570 kb on - strand, within GFF1375 at 24.607 kb on + strand at 24.618 kb on - strand at 24.756 kb on + strand, within GFF1376 at 24.847 kb on + strand, within GFF1376 at 25.143 kb on + strand, within GFF1376 at 25.144 kb on - strand, within GFF1376 at 25.233 kb on + strand, within GFF1376 at 25.233 kb on + strand, within GFF1376 at 25.234 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.238 kb on - strand, within GFF1376 at 25.257 kb on + strand, within GFF1376 at 25.334 kb on + strand, within GFF1376 at 25.334 kb on + strand, within GFF1376 at 25.335 kb on - strand, within GFF1376
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 23,065 + GFF1373 0.11 -1.9 23,185 - GFF1373 0.22 +1.0 23,236 - GFF1373 0.26 -0.5 23,442 - GFF1373 0.45 +0.8 23,488 - GFF1373 0.49 +1.8 23,488 - GFF1373 0.49 -1.0 23,488 - GFF1373 0.49 +2.5 23,488 - GFF1373 0.49 -0.3 23,558 + GFF1373 0.55 -1.3 23,644 + GFF1373 0.63 +0.8 23,645 - GFF1373 0.63 +1.2 23,990 + +0.0 24,036 + -1.1 24,061 - +0.5 24,313 - GFF1374 0.73 -3.8 24,346 + GFF1374 0.82 -1.5 24,445 - +0.9 24,445 - -3.1 24,465 + +2.5 24,570 - GFF1375 0.80 -0.8 24,607 + +1.6 24,618 - +0.5 24,756 + GFF1376 0.12 -0.8 24,847 + GFF1376 0.18 -2.0 25,143 + GFF1376 0.37 +0.7 25,144 - GFF1376 0.37 -0.6 25,233 + GFF1376 0.43 -0.1 25,233 + GFF1376 0.43 -0.5 25,234 - GFF1376 0.43 -0.2 25,238 - GFF1376 0.43 +1.8 25,238 - GFF1376 0.43 +3.2 25,238 - GFF1376 0.43 +1.0 25,257 + GFF1376 0.44 -0.2 25,334 + GFF1376 0.49 +0.7 25,334 + GFF1376 0.49 -1.1 25,335 - GFF1376 0.49 -3.7
Or see this region's nucleotide sequence