Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1327 and GFF1328 are separated by 56 nucleotides GFF1328 and GFF1329 overlap by 10 nucleotides GFF1329 and GFF1330 are separated by 268 nucleotides
GFF1327 - 2-deoxy-D-gluconate 3-dehydrogenase (EC 1.1.1.125), at 36,382 to 37,143
GFF1327
GFF1328 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 37,200 to 38,036
GFF1328
GFF1329 - 4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase (EC 5.3.1.17), at 38,027 to 38,197
GFF1329
GFF1330 - 3-ketoacyl-CoA thiolase (EC 2.3.1.16) @ Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 38,466 to 39,644
GFF1330
Position (kb) 37
38
39 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 36.247 kb on + strand at 36.279 kb on + strand at 36.406 kb on + strand at 36.407 kb on - strand at 36.407 kb on - strand at 36.407 kb on - strand at 36.418 kb on - strand at 36.420 kb on - strand at 36.509 kb on - strand, within GFF1327 at 36.529 kb on - strand, within GFF1327 at 36.573 kb on + strand, within GFF1327 at 36.573 kb on + strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.574 kb on - strand, within GFF1327 at 36.576 kb on - strand, within GFF1327 at 36.604 kb on - strand, within GFF1327 at 36.718 kb on - strand, within GFF1327 at 36.777 kb on + strand, within GFF1327 at 36.778 kb on - strand, within GFF1327 at 36.889 kb on - strand, within GFF1327 at 37.000 kb on - strand, within GFF1327 at 37.279 kb on + strand at 37.306 kb on - strand, within GFF1328 at 37.306 kb on - strand, within GFF1328 at 37.382 kb on + strand, within GFF1328 at 37.383 kb on - strand, within GFF1328 at 37.423 kb on + strand, within GFF1328 at 37.471 kb on - strand, within GFF1328 at 37.540 kb on + strand, within GFF1328 at 37.550 kb on + strand, within GFF1328 at 37.551 kb on - strand, within GFF1328 at 37.721 kb on + strand, within GFF1328 at 37.723 kb on + strand, within GFF1328 at 37.793 kb on + strand, within GFF1328 at 37.794 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.845 kb on - strand, within GFF1328 at 37.902 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.905 kb on - strand, within GFF1328 at 37.922 kb on - strand, within GFF1328 at 37.932 kb on - strand, within GFF1328 at 37.953 kb on - strand at 38.019 kb on - strand at 38.188 kb on + strand at 38.708 kb on + strand, within GFF1330 at 38.764 kb on + strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.765 kb on - strand, within GFF1330 at 38.767 kb on - strand, within GFF1330 at 38.913 kb on - strand, within GFF1330 at 38.931 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 38.936 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330 at 39.026 kb on + strand, within GFF1330
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 36,247 + +0.9 36,279 + -1.9 36,406 + +0.4 36,407 - -3.0 36,407 - -0.5 36,407 - +0.4 36,418 - +1.3 36,420 - +0.6 36,509 - GFF1327 0.17 -0.9 36,529 - GFF1327 0.19 -2.8 36,573 + GFF1327 0.25 +0.0 36,573 + GFF1327 0.25 +0.1 36,574 - GFF1327 0.25 +1.0 36,574 - GFF1327 0.25 +0.2 36,574 - GFF1327 0.25 +1.5 36,576 - GFF1327 0.25 +0.1 36,604 - GFF1327 0.29 -0.5 36,718 - GFF1327 0.44 -0.4 36,777 + GFF1327 0.52 -0.3 36,778 - GFF1327 0.52 +0.1 36,889 - GFF1327 0.67 +0.4 37,000 - GFF1327 0.81 -0.1 37,279 + -2.9 37,306 - GFF1328 0.13 -0.1 37,306 - GFF1328 0.13 +0.7 37,382 + GFF1328 0.22 +0.5 37,383 - GFF1328 0.22 -0.9 37,423 + GFF1328 0.27 -0.1 37,471 - GFF1328 0.32 +0.3 37,540 + GFF1328 0.41 -2.7 37,550 + GFF1328 0.42 -2.4 37,551 - GFF1328 0.42 +0.3 37,721 + GFF1328 0.62 -0.3 37,723 + GFF1328 0.62 +0.7 37,793 + GFF1328 0.71 +0.7 37,794 - GFF1328 0.71 +0.7 37,845 - GFF1328 0.77 -1.8 37,845 - GFF1328 0.77 -0.2 37,845 - GFF1328 0.77 -2.5 37,902 - GFF1328 0.84 -0.0 37,905 - GFF1328 0.84 +0.6 37,905 - GFF1328 0.84 -1.8 37,922 - GFF1328 0.86 +0.3 37,932 - GFF1328 0.87 +0.5 37,953 - +0.8 38,019 - +1.0 38,188 + +0.4 38,708 + GFF1330 0.21 +0.1 38,764 + GFF1330 0.25 -2.7 38,765 - GFF1330 0.25 +1.2 38,765 - GFF1330 0.25 -0.4 38,765 - GFF1330 0.25 +0.0 38,765 - GFF1330 0.25 +0.7 38,767 - GFF1330 0.26 -0.2 38,913 - GFF1330 0.38 +1.1 38,931 + GFF1330 0.39 +1.2 38,936 + GFF1330 0.40 +1.6 38,936 + GFF1330 0.40 -2.4 39,026 + GFF1330 0.47 -1.3 39,026 + GFF1330 0.47 -0.7
Or see this region's nucleotide sequence