Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1154 and GFF1155 are separated by 362 nucleotides GFF1155 and GFF1156 are separated by 14 nucleotides GFF1156 and GFF1157 are separated by 21 nucleotides GFF1157 and GFF1158 overlap by 14 nucleotides
GFF1154 - IncF plasmid conjugative transfer regulator TraJ, at 8,186 to 8,557
GFF1154
GFF1155 - IncF plasmid conjugative transfer pilin protein TraA, at 8,920 to 9,282
GFF1155
GFF1156 - IncF plasmid conjugative transfer pilus assembly protein TraL, at 9,297 to 9,608
GFF1156
GFF1157 - IncF plasmid conjugative transfer pilus assembly protein TraE, at 9,630 to 10,196
GFF1157
GFF1158 - IncF plasmid conjugative transfer pilus assembly protein TraK, at 10,183 to 10,923
GFF1158
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 7.946 kb on - strand at 8.021 kb on - strand at 8.063 kb on - strand at 8.067 kb on - strand at 8.068 kb on + strand at 8.877 kb on - strand at 8.919 kb on + strand at 8.982 kb on + strand, within GFF1155 at 8.982 kb on + strand, within GFF1155 at 8.982 kb on + strand, within GFF1155 at 9.194 kb on - strand, within GFF1155 at 9.194 kb on - strand, within GFF1155 at 9.261 kb on + strand at 9.290 kb on + strand at 9.327 kb on + strand at 9.389 kb on - strand, within GFF1156 at 9.537 kb on - strand, within GFF1156 at 9.537 kb on - strand, within GFF1156 at 9.602 kb on + strand at 9.740 kb on + strand, within GFF1157 at 9.783 kb on - strand, within GFF1157 at 9.783 kb on - strand, within GFF1157 at 9.791 kb on + strand, within GFF1157 at 9.834 kb on + strand, within GFF1157 at 9.835 kb on - strand, within GFF1157 at 9.838 kb on + strand, within GFF1157 at 9.924 kb on + strand, within GFF1157 at 9.925 kb on - strand, within GFF1157 at 9.925 kb on - strand, within GFF1157 at 10.001 kb on + strand, within GFF1157 at 10.045 kb on - strand, within GFF1157 at 10.120 kb on - strand, within GFF1157 at 10.201 kb on + strand at 10.202 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 7,946 - -1.6 8,021 - -3.4 8,063 - -0.7 8,067 - +1.1 8,068 + +0.1 8,877 - +0.4 8,919 + +0.1 8,982 + GFF1155 0.17 -0.4 8,982 + GFF1155 0.17 -0.6 8,982 + GFF1155 0.17 -3.5 9,194 - GFF1155 0.75 -2.1 9,194 - GFF1155 0.75 -0.9 9,261 + -0.3 9,290 + +1.1 9,327 + -0.8 9,389 - GFF1156 0.29 +0.6 9,537 - GFF1156 0.77 -1.9 9,537 - GFF1156 0.77 +1.1 9,602 + -0.0 9,740 + GFF1157 0.19 -1.9 9,783 - GFF1157 0.27 -0.1 9,783 - GFF1157 0.27 +0.9 9,791 + GFF1157 0.28 -0.5 9,834 + GFF1157 0.36 +1.2 9,835 - GFF1157 0.36 -0.5 9,838 + GFF1157 0.37 -2.4 9,924 + GFF1157 0.52 +0.2 9,925 - GFF1157 0.52 -0.9 9,925 - GFF1157 0.52 +0.4 10,001 + GFF1157 0.65 +0.3 10,045 - GFF1157 0.73 -0.2 10,120 - GFF1157 0.86 -1.9 10,201 + -0.6 10,202 - +0.6
Or see this region's nucleotide sequence