Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1129 and GFF1130 are separated by 199 nucleotides GFF1130 and GFF1131 are separated by 15 nucleotides GFF1131 and GFF1132 are separated by 21 nucleotides
GFF1129 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.1.2), at 318,580 to 319,530
GFF1129
GFF1130 - Putative nitrite reductase, at 319,730 to 320,932
GFF1130
GFF1131 - Inosine-uridine preferring nucleoside hydrolase (EC 3.2.2.1), at 320,948 to 321,868
GFF1131
GFF1132 - Transcriptional regulatory protein CitB, DpiA, at 321,890 to 322,576
GFF1132
Position (kb) 319
320
321 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 319.528 kb on + strand at 319.564 kb on + strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.619 kb on + strand at 319.643 kb on + strand at 319.707 kb on + strand at 319.843 kb on + strand at 319.871 kb on - strand, within GFF1130 at 319.895 kb on - strand, within GFF1130 at 319.904 kb on + strand, within GFF1130 at 319.905 kb on - strand, within GFF1130 at 319.910 kb on + strand, within GFF1130 at 320.042 kb on - strand, within GFF1130 at 320.097 kb on - strand, within GFF1130 at 320.159 kb on + strand, within GFF1130 at 320.239 kb on - strand, within GFF1130 at 320.247 kb on + strand, within GFF1130 at 320.247 kb on + strand, within GFF1130 at 320.282 kb on - strand, within GFF1130 at 320.335 kb on - strand, within GFF1130 at 320.382 kb on - strand, within GFF1130 at 320.402 kb on + strand, within GFF1130 at 320.402 kb on + strand, within GFF1130 at 320.403 kb on - strand, within GFF1130 at 320.403 kb on - strand, within GFF1130 at 320.516 kb on + strand, within GFF1130 at 320.516 kb on + strand, within GFF1130 at 320.597 kb on + strand, within GFF1130 at 320.597 kb on + strand, within GFF1130 at 320.606 kb on + strand, within GFF1130 at 320.638 kb on + strand, within GFF1130 at 320.638 kb on + strand, within GFF1130 at 320.642 kb on + strand, within GFF1130 at 320.650 kb on - strand, within GFF1130 at 320.652 kb on + strand, within GFF1130 at 320.657 kb on - strand, within GFF1130 at 320.848 kb on + strand at 320.848 kb on + strand at 320.854 kb on - strand at 320.920 kb on + strand at 320.930 kb on + strand at 320.962 kb on - strand at 321.189 kb on - strand, within GFF1131 at 321.265 kb on + strand, within GFF1131 at 321.312 kb on + strand, within GFF1131 at 321.337 kb on + strand, within GFF1131 at 321.348 kb on + strand, within GFF1131 at 321.349 kb on - strand, within GFF1131 at 321.427 kb on + strand, within GFF1131 at 321.625 kb on + strand, within GFF1131 at 321.870 kb on + strand at 321.871 kb on - strand at 321.907 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 319,528 + -2.2 319,564 + +0.4 319,619 + -0.5 319,619 + -0.6 319,619 + -1.0 319,643 + -1.2 319,707 + +0.2 319,843 + -1.1 319,871 - GFF1130 0.12 +0.4 319,895 - GFF1130 0.14 -1.0 319,904 + GFF1130 0.14 +2.5 319,905 - GFF1130 0.15 -0.3 319,910 + GFF1130 0.15 +1.1 320,042 - GFF1130 0.26 +1.3 320,097 - GFF1130 0.31 +0.5 320,159 + GFF1130 0.36 -1.1 320,239 - GFF1130 0.42 -0.7 320,247 + GFF1130 0.43 +1.3 320,247 + GFF1130 0.43 +0.7 320,282 - GFF1130 0.46 -0.4 320,335 - GFF1130 0.50 -0.5 320,382 - GFF1130 0.54 +0.6 320,402 + GFF1130 0.56 -0.3 320,402 + GFF1130 0.56 -0.4 320,403 - GFF1130 0.56 -3.4 320,403 - GFF1130 0.56 +0.1 320,516 + GFF1130 0.65 +0.4 320,516 + GFF1130 0.65 -0.9 320,597 + GFF1130 0.72 -0.6 320,597 + GFF1130 0.72 -0.3 320,606 + GFF1130 0.73 -0.7 320,638 + GFF1130 0.75 +0.4 320,638 + GFF1130 0.75 -0.1 320,642 + GFF1130 0.76 +0.3 320,650 - GFF1130 0.76 -0.7 320,652 + GFF1130 0.77 -0.2 320,657 - GFF1130 0.77 -1.0 320,848 + -0.5 320,848 + -1.8 320,854 - +0.3 320,920 + -0.3 320,930 + -1.5 320,962 - +0.2 321,189 - GFF1131 0.26 +0.1 321,265 + GFF1131 0.34 +0.0 321,312 + GFF1131 0.40 -2.3 321,337 + GFF1131 0.42 -0.2 321,348 + GFF1131 0.43 +2.0 321,349 - GFF1131 0.44 +0.6 321,427 + GFF1131 0.52 -1.9 321,625 + GFF1131 0.74 +1.5 321,870 + +0.3 321,871 - +0.4 321,907 - +1.0
Or see this region's nucleotide sequence