Experiment: LB_plus_SM_buffer with P22_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1060 and GFF1061 are separated by 57 nucleotides GFF1061 and GFF1062 are separated by 101 nucleotides GFF1062 and GFF1063 are separated by 48 nucleotides
GFF1060 - Soluble lytic murein transglycosylase precursor (EC 3.2.1.-), at 242,387 to 244,324
GFF1060
GFF1061 - Transcriptional repressor protein TrpR, at 244,382 to 244,708
GFF1061
GFF1062 - Inosine/xanthosine triphosphatase (EC 3.6.1.-), at 244,810 to 245,325
GFF1062
GFF1063 - Phosphoglycerate mutase (EC 5.4.2.1), at 245,374 to 246,021
GFF1063
Position (kb) 244
245 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 243.382 kb on + strand, within GFF1060 at 243.502 kb on - strand, within GFF1060 at 243.645 kb on - strand, within GFF1060 at 243.827 kb on - strand, within GFF1060 at 243.845 kb on + strand, within GFF1060 at 243.875 kb on + strand, within GFF1060 at 243.876 kb on - strand, within GFF1060 at 243.876 kb on - strand, within GFF1060 at 243.959 kb on - strand, within GFF1060 at 244.008 kb on - strand, within GFF1060 at 244.064 kb on + strand, within GFF1060 at 244.073 kb on + strand, within GFF1060 at 244.080 kb on - strand, within GFF1060 at 244.080 kb on - strand, within GFF1060 at 244.080 kb on - strand, within GFF1060 at 244.124 kb on - strand, within GFF1060 at 244.247 kb on + strand at 244.256 kb on + strand at 244.290 kb on + strand at 244.336 kb on + strand at 244.337 kb on - strand at 244.337 kb on - strand at 244.376 kb on + strand at 244.426 kb on + strand, within GFF1061 at 244.455 kb on + strand, within GFF1061 at 244.495 kb on - strand, within GFF1061 at 244.552 kb on + strand, within GFF1061 at 244.553 kb on - strand, within GFF1061 at 244.718 kb on - strand at 244.796 kb on + strand at 244.797 kb on - strand at 244.865 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 244.938 kb on + strand, within GFF1062 at 245.146 kb on - strand, within GFF1062 at 245.230 kb on - strand, within GFF1062 at 245.233 kb on - strand, within GFF1062 at 245.386 kb on + strand at 245.523 kb on + strand, within GFF1063 at 245.540 kb on + strand, within GFF1063
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with P22_phage 15 MOI remove 243,382 + GFF1060 0.51 -0.6 243,502 - GFF1060 0.58 +0.9 243,645 - GFF1060 0.65 -1.7 243,827 - GFF1060 0.74 +0.6 243,845 + GFF1060 0.75 +0.5 243,875 + GFF1060 0.77 +0.1 243,876 - GFF1060 0.77 +1.2 243,876 - GFF1060 0.77 +0.9 243,959 - GFF1060 0.81 -1.0 244,008 - GFF1060 0.84 -0.4 244,064 + GFF1060 0.87 +0.7 244,073 + GFF1060 0.87 +0.0 244,080 - GFF1060 0.87 +0.3 244,080 - GFF1060 0.87 -2.7 244,080 - GFF1060 0.87 +0.1 244,124 - GFF1060 0.90 +0.4 244,247 + +1.2 244,256 + -0.5 244,290 + +0.3 244,336 + +0.1 244,337 - +1.5 244,337 - +0.8 244,376 + +2.4 244,426 + GFF1061 0.13 +0.6 244,455 + GFF1061 0.22 +2.2 244,495 - GFF1061 0.35 -0.7 244,552 + GFF1061 0.52 +1.0 244,553 - GFF1061 0.52 -1.7 244,718 - +0.4 244,796 + -1.5 244,797 - +0.7 244,865 + GFF1062 0.11 -0.3 244,938 + GFF1062 0.25 +1.0 244,938 + GFF1062 0.25 +1.3 244,938 + GFF1062 0.25 -1.2 245,146 - GFF1062 0.65 +1.2 245,230 - GFF1062 0.81 +1.1 245,233 - GFF1062 0.82 +2.5 245,386 + +2.9 245,523 + GFF1063 0.23 -0.0 245,540 + GFF1063 0.26 +1.0
Or see this region's nucleotide sequence