Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF967 and GFF968 are separated by 186 nucleotides GFF968 and GFF969 are separated by 489 nucleotides GFF969 and GFF970 are separated by 97 nucleotides GFF970 and GFF971 are separated by 330 nucleotides
GFF967 - putative inner membrane protein, at 154,589 to 155,197
GFF967
GFF968 - hypothetical protein, at 155,384 to 155,527
GFF968
GFF969 - FIG007491: hypothetical protein YeeN, at 156,017 to 156,733
GFF969
GFF970 - hypothetical protein, at 156,831 to 156,962
GFF970
GFF971 - 3-demethylubiquinone-9 3-methyltransferase (EC 2.1.1.64), at 157,293 to 158,918
GFF971
Position (kb) 156
157 Strain fitness (log2 ratio) -1
0
1
2 at 155.033 kb on - strand, within GFF967 at 155.059 kb on + strand, within GFF967 at 155.060 kb on - strand, within GFF967 at 155.104 kb on + strand, within GFF967 at 155.197 kb on + strand at 155.201 kb on + strand at 155.219 kb on - strand at 155.251 kb on - strand at 155.458 kb on + strand, within GFF968 at 155.463 kb on - strand, within GFF968 at 155.544 kb on + strand at 155.604 kb on + strand at 155.604 kb on + strand at 155.699 kb on + strand at 155.754 kb on + strand at 155.754 kb on + strand at 155.783 kb on + strand at 155.838 kb on + strand at 155.853 kb on - strand at 156.484 kb on - strand, within GFF969 at 157.262 kb on - strand at 157.277 kb on + strand at 157.456 kb on + strand, within GFF971 at 157.456 kb on + strand, within GFF971 at 157.457 kb on - strand, within GFF971 at 157.457 kb on - strand, within GFF971 at 157.616 kb on - strand, within GFF971 at 157.616 kb on - strand, within GFF971 at 157.672 kb on - strand, within GFF971 at 157.672 kb on - strand, within GFF971
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 155,033 - GFF967 0.73 +0.2 155,059 + GFF967 0.77 +0.5 155,060 - GFF967 0.77 -1.0 155,104 + GFF967 0.85 -0.1 155,197 + -1.0 155,201 + -0.6 155,219 - -0.4 155,251 - -0.4 155,458 + GFF968 0.51 -1.0 155,463 - GFF968 0.55 +0.6 155,544 + -1.3 155,604 + +0.4 155,604 + -0.3 155,699 + +0.1 155,754 + -0.2 155,754 + -0.1 155,783 + +0.4 155,838 + +0.1 155,853 - -0.5 156,484 - GFF969 0.65 -0.7 157,262 - -0.5 157,277 + -0.5 157,456 + GFF971 0.10 -1.1 157,456 + GFF971 0.10 -1.3 157,457 - GFF971 0.10 -0.1 157,457 - GFF971 0.10 +0.3 157,616 - GFF971 0.20 +0.9 157,616 - GFF971 0.20 +2.0 157,672 - GFF971 0.23 +0.1 157,672 - GFF971 0.23 +0.3
Or see this region's nucleotide sequence