Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF830 and GFF831 are separated by 143 nucleotides GFF831 and GFF832 are separated by 37 nucleotides GFF832 and GFF833 are separated by 90 nucleotides GFF833 and GFF834 are separated by 131 nucleotides
GFF830 - Adenylosuccinate synthetase (EC 6.3.4.4), at 12,445 to 13,743
GFF830
GFF831 - Nitrite-sensitive transcriptional repressor NsrR, at 13,887 to 14,375
GFF831
GFF832 - '3'-to-5' exoribonuclease RNase R' transl_table=11, at 14,413 to 16,851
GFF832
GFF833 - '23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-)' transl_table=11, at 16,942 to 17,673
GFF833
GFF834 - FIG006163: hypothetical protein, at 17,805 to 18,209
GFF834
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -1
0
1
2 at 13.791 kb on + strand at 13.871 kb on - strand at 13.871 kb on - strand at 13.909 kb on - strand at 13.911 kb on - strand at 13.911 kb on - strand at 13.981 kb on + strand, within GFF831 at 13.982 kb on - strand, within GFF831 at 13.996 kb on - strand, within GFF831 at 13.996 kb on - strand, within GFF831 at 14.052 kb on + strand, within GFF831 at 14.223 kb on - strand, within GFF831 at 14.313 kb on + strand, within GFF831 at 14.548 kb on - strand at 14.638 kb on + strand at 14.708 kb on + strand, within GFF832 at 14.738 kb on - strand, within GFF832 at 14.766 kb on + strand, within GFF832 at 15.025 kb on - strand, within GFF832 at 15.063 kb on + strand, within GFF832 at 15.110 kb on + strand, within GFF832 at 15.116 kb on - strand, within GFF832 at 15.116 kb on - strand, within GFF832 at 15.322 kb on + strand, within GFF832 at 15.323 kb on - strand, within GFF832 at 15.325 kb on + strand, within GFF832 at 15.325 kb on + strand, within GFF832 at 15.325 kb on + strand, within GFF832 at 15.325 kb on + strand, within GFF832 at 15.326 kb on - strand, within GFF832 at 15.326 kb on - strand, within GFF832 at 15.330 kb on - strand, within GFF832 at 15.468 kb on + strand, within GFF832 at 15.469 kb on - strand, within GFF832 at 15.576 kb on + strand, within GFF832 at 15.576 kb on + strand, within GFF832 at 15.780 kb on + strand, within GFF832 at 15.787 kb on - strand, within GFF832 at 15.843 kb on + strand, within GFF832 at 15.857 kb on + strand, within GFF832 at 15.874 kb on - strand, within GFF832 at 15.911 kb on + strand, within GFF832 at 16.099 kb on + strand, within GFF832 at 16.099 kb on + strand, within GFF832 at 16.162 kb on + strand, within GFF832 at 16.163 kb on - strand, within GFF832 at 16.453 kb on + strand, within GFF832 at 16.609 kb on - strand at 16.665 kb on + strand at 17.303 kb on + strand, within GFF833 at 17.303 kb on + strand, within GFF833 at 17.304 kb on - strand, within GFF833 at 17.304 kb on - strand, within GFF833 at 17.481 kb on + strand, within GFF833 at 17.545 kb on - strand, within GFF833
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 13,791 + +0.2 13,871 - -0.8 13,871 - -0.1 13,909 - -0.9 13,911 - +0.4 13,911 - +0.4 13,981 + GFF831 0.19 +0.7 13,982 - GFF831 0.19 -0.1 13,996 - GFF831 0.22 -1.5 13,996 - GFF831 0.22 +0.2 14,052 + GFF831 0.34 +0.3 14,223 - GFF831 0.69 -0.1 14,313 + GFF831 0.87 -0.7 14,548 - +1.3 14,638 + +1.9 14,708 + GFF832 0.12 +1.4 14,738 - GFF832 0.13 +0.7 14,766 + GFF832 0.14 +2.0 15,025 - GFF832 0.25 +0.5 15,063 + GFF832 0.27 +1.6 15,110 + GFF832 0.29 +1.6 15,116 - GFF832 0.29 +1.1 15,116 - GFF832 0.29 +1.8 15,322 + GFF832 0.37 +1.9 15,323 - GFF832 0.37 +1.9 15,325 + GFF832 0.37 +0.5 15,325 + GFF832 0.37 +1.0 15,325 + GFF832 0.37 +1.1 15,325 + GFF832 0.37 +0.4 15,326 - GFF832 0.37 +1.2 15,326 - GFF832 0.37 +0.6 15,330 - GFF832 0.38 +1.4 15,468 + GFF832 0.43 +1.5 15,469 - GFF832 0.43 +0.8 15,576 + GFF832 0.48 +1.1 15,576 + GFF832 0.48 +1.7 15,780 + GFF832 0.56 +0.8 15,787 - GFF832 0.56 +2.1 15,843 + GFF832 0.59 +1.5 15,857 + GFF832 0.59 +0.8 15,874 - GFF832 0.60 -0.1 15,911 + GFF832 0.61 +1.3 16,099 + GFF832 0.69 +1.5 16,099 + GFF832 0.69 +1.8 16,162 + GFF832 0.72 +1.4 16,163 - GFF832 0.72 +0.2 16,453 + GFF832 0.84 +0.7 16,609 - +0.8 16,665 + +0.7 17,303 + GFF833 0.49 -0.6 17,303 + GFF833 0.49 +0.9 17,304 - GFF833 0.49 +2.0 17,304 - GFF833 0.49 +0.1 17,481 + GFF833 0.74 +0.0 17,545 - GFF833 0.82 +0.8
Or see this region's nucleotide sequence