Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF803 and GFF804 are separated by 88 nucleotides GFF804 and GFF805 overlap by 8 nucleotides GFF805 and GFF806 are separated by 3 nucleotides GFF806 and GFF807 overlap by 4 nucleotides GFF807 and GFF808 are separated by 10 nucleotides
GFF803 - Prophage Clp protease-like protein, at 150,438 to 152,513
GFF803
GFF804 - putative RecA/RadA recombinase, at 152,602 to 152,925
GFF804
GFF805 - Phage protein, at 152,918 to 153,193
GFF805
GFF806 - Phage tail completion protein, at 153,197 to 153,781
GFF806
GFF807 - Phage minor tail protein, at 153,778 to 154,179
GFF807
GFF808 - Phage major tail protein, at 154,190 to 154,933
GFF808
Position (kb) 152
153
154 Strain fitness (log2 ratio) -1
0
1 at 151.998 kb on + strand, within GFF803 at 152.265 kb on - strand, within GFF803 at 152.452 kb on + strand at 152.529 kb on - strand at 152.618 kb on + strand at 152.618 kb on + strand at 152.619 kb on - strand at 152.619 kb on - strand at 153.032 kb on - strand, within GFF805 at 153.092 kb on + strand, within GFF805 at 153.253 kb on + strand at 153.554 kb on + strand, within GFF806 at 153.574 kb on + strand, within GFF806 at 153.608 kb on - strand, within GFF806 at 153.706 kb on + strand, within GFF806 at 153.741 kb on - strand at 154.017 kb on + strand, within GFF807 at 154.078 kb on - strand, within GFF807 at 154.083 kb on + strand, within GFF807 at 154.109 kb on - strand, within GFF807 at 154.169 kb on - strand at 154.183 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 151,998 + GFF803 0.75 -1.7 152,265 - GFF803 0.88 -0.3 152,452 + -0.2 152,529 - -0.4 152,618 + +0.1 152,618 + -0.0 152,619 - -0.1 152,619 - -0.6 153,032 - GFF805 0.41 -0.3 153,092 + GFF805 0.63 -1.1 153,253 + -0.7 153,554 + GFF806 0.61 +0.7 153,574 + GFF806 0.64 -0.0 153,608 - GFF806 0.70 +1.0 153,706 + GFF806 0.87 +0.4 153,741 - +0.5 154,017 + GFF807 0.59 +0.2 154,078 - GFF807 0.75 +1.0 154,083 + GFF807 0.76 -1.0 154,109 - GFF807 0.82 +0.1 154,169 - +0.3 154,183 + -0.1
Or see this region's nucleotide sequence