Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF579 and GFF580 are separated by 177 nucleotides GFF580 and GFF581 are separated by 43 nucleotides GFF581 and GFF582 are separated by 43 nucleotides
GFF579 - Glycogen phosphorylase (EC 2.4.1.1), at 311,719 to 314,166
GFF579
GFF580 - Transcriptional regulator, ArsR family, at 314,344 to 315,099
GFF580
GFF581 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 315,143 to 316,048
GFF581
GFF582 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 316,092 to 317,807
GFF582
Position (kb) 315
316
317 Strain fitness (log2 ratio) -1
0
1
2 at 314.216 kb on + strand at 314.312 kb on - strand at 314.393 kb on + strand at 314.401 kb on + strand at 314.511 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.531 kb on - strand, within GFF580 at 314.562 kb on + strand, within GFF580 at 314.581 kb on + strand, within GFF580 at 314.582 kb on - strand, within GFF580 at 314.676 kb on + strand, within GFF580 at 314.677 kb on - strand, within GFF580 at 314.709 kb on + strand, within GFF580 at 314.747 kb on + strand, within GFF580 at 314.747 kb on + strand, within GFF580 at 314.748 kb on - strand, within GFF580 at 314.751 kb on + strand, within GFF580 at 314.751 kb on + strand, within GFF580 at 314.829 kb on - strand, within GFF580 at 314.915 kb on - strand, within GFF580 at 315.013 kb on - strand, within GFF580 at 315.051 kb on + strand at 315.076 kb on - strand at 315.162 kb on - strand at 315.218 kb on + strand at 315.219 kb on - strand at 315.294 kb on + strand, within GFF581 at 315.299 kb on - strand, within GFF581 at 315.369 kb on + strand, within GFF581 at 315.384 kb on - strand, within GFF581 at 315.432 kb on - strand, within GFF581 at 315.436 kb on + strand, within GFF581 at 315.436 kb on + strand, within GFF581 at 315.451 kb on + strand, within GFF581 at 315.452 kb on - strand, within GFF581 at 315.462 kb on + strand, within GFF581 at 315.506 kb on - strand, within GFF581 at 315.535 kb on + strand, within GFF581 at 315.590 kb on - strand, within GFF581 at 315.657 kb on - strand, within GFF581 at 315.691 kb on + strand, within GFF581 at 315.802 kb on + strand, within GFF581 at 315.802 kb on + strand, within GFF581 at 315.805 kb on + strand, within GFF581 at 315.806 kb on - strand, within GFF581 at 315.809 kb on + strand, within GFF581 at 315.813 kb on - strand, within GFF581 at 315.813 kb on - strand, within GFF581 at 315.824 kb on - strand, within GFF581 at 315.835 kb on + strand, within GFF581 at 315.915 kb on - strand, within GFF581 at 316.020 kb on - strand at 316.050 kb on + strand at 316.051 kb on - strand at 316.093 kb on + strand at 316.094 kb on - strand at 316.098 kb on - strand at 316.117 kb on - strand at 316.251 kb on + strand at 316.434 kb on + strand, within GFF582 at 316.583 kb on + strand, within GFF582 at 316.620 kb on - strand, within GFF582 at 316.656 kb on + strand, within GFF582 at 316.694 kb on + strand, within GFF582 at 316.752 kb on - strand, within GFF582 at 316.894 kb on + strand, within GFF582 at 316.894 kb on + strand, within GFF582 at 317.030 kb on + strand, within GFF582
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 314,216 + -0.4 314,312 - +0.2 314,393 + -0.7 314,401 + +0.1 314,511 - GFF580 0.22 -0.1 314,531 - GFF580 0.25 -0.5 314,531 - GFF580 0.25 -0.8 314,531 - GFF580 0.25 +0.5 314,531 - GFF580 0.25 -0.6 314,531 - GFF580 0.25 -0.1 314,531 - GFF580 0.25 -0.7 314,562 + GFF580 0.29 -0.3 314,581 + GFF580 0.31 -0.1 314,582 - GFF580 0.31 +0.1 314,676 + GFF580 0.44 -0.4 314,677 - GFF580 0.44 -0.4 314,709 + GFF580 0.48 +0.6 314,747 + GFF580 0.53 -0.0 314,747 + GFF580 0.53 -0.8 314,748 - GFF580 0.53 -0.2 314,751 + GFF580 0.54 +0.1 314,751 + GFF580 0.54 +1.2 314,829 - GFF580 0.64 -0.4 314,915 - GFF580 0.76 +2.5 315,013 - GFF580 0.88 -0.3 315,051 + +1.2 315,076 - -0.3 315,162 - -0.4 315,218 + -0.3 315,219 - +0.7 315,294 + GFF581 0.17 -0.2 315,299 - GFF581 0.17 -0.6 315,369 + GFF581 0.25 -0.5 315,384 - GFF581 0.27 +0.2 315,432 - GFF581 0.32 -0.1 315,436 + GFF581 0.32 +0.6 315,436 + GFF581 0.32 +0.2 315,451 + GFF581 0.34 -0.4 315,452 - GFF581 0.34 +0.3 315,462 + GFF581 0.35 -0.8 315,506 - GFF581 0.40 -0.8 315,535 + GFF581 0.43 +0.5 315,590 - GFF581 0.49 +0.6 315,657 - GFF581 0.57 -0.7 315,691 + GFF581 0.60 -1.0 315,802 + GFF581 0.73 +0.1 315,802 + GFF581 0.73 -1.4 315,805 + GFF581 0.73 +0.0 315,806 - GFF581 0.73 -1.1 315,809 + GFF581 0.74 -0.2 315,813 - GFF581 0.74 -0.1 315,813 - GFF581 0.74 +0.4 315,824 - GFF581 0.75 -0.4 315,835 + GFF581 0.76 +0.5 315,915 - GFF581 0.85 -0.7 316,020 - +0.1 316,050 + +0.5 316,051 - +0.5 316,093 + +0.0 316,094 - +1.2 316,098 - -0.5 316,117 - +0.2 316,251 + -0.4 316,434 + GFF582 0.20 -1.0 316,583 + GFF582 0.29 -0.7 316,620 - GFF582 0.31 -0.3 316,656 + GFF582 0.33 -0.8 316,694 + GFF582 0.35 -0.6 316,752 - GFF582 0.38 -0.3 316,894 + GFF582 0.47 -0.5 316,894 + GFF582 0.47 -0.5 317,030 + GFF582 0.55 -0.1
Or see this region's nucleotide sequence