Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF498 and GFF499 are separated by 58 nucleotides GFF499 and GFF500 are separated by 109 nucleotides GFF500 and GFF501 are separated by 39 nucleotides
GFF498 - hypothetical protein, at 225,552 to 225,668
GFF498
GFF499 - GGDEF/EAL domain protein YhjH, at 225,727 to 226,494
GFF499
GFF500 - Uncharacterized protein YhjG, at 226,604 to 228,664
GFF500
GFF501 - Inner membrane metabolite transport protein YhjE, at 228,704 to 230,026
GFF501
Position (kb) 226
227
228
229 Strain fitness (log2 ratio) -2
-1
0
1 at 225.732 kb on - strand at 225.732 kb on - strand at 225.752 kb on + strand at 225.771 kb on + strand at 225.820 kb on + strand, within GFF499 at 225.826 kb on + strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.839 kb on - strand, within GFF499 at 225.912 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.025 kb on + strand, within GFF499 at 226.107 kb on - strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.137 kb on + strand, within GFF499 at 226.261 kb on - strand, within GFF499 at 226.521 kb on + strand at 226.524 kb on + strand at 226.525 kb on - strand at 226.525 kb on - strand at 226.610 kb on - strand at 226.665 kb on + strand at 227.018 kb on - strand, within GFF500 at 227.051 kb on - strand, within GFF500 at 227.276 kb on + strand, within GFF500 at 227.311 kb on - strand, within GFF500 at 227.475 kb on + strand, within GFF500 at 227.477 kb on + strand, within GFF500 at 227.568 kb on + strand, within GFF500 at 227.632 kb on + strand, within GFF500 at 227.632 kb on + strand, within GFF500 at 227.633 kb on - strand, within GFF500 at 227.686 kb on - strand, within GFF500 at 227.940 kb on + strand, within GFF500 at 227.940 kb on + strand, within GFF500 at 227.946 kb on + strand, within GFF500 at 227.980 kb on + strand, within GFF500 at 227.980 kb on + strand, within GFF500 at 228.039 kb on + strand, within GFF500 at 228.039 kb on + strand, within GFF500 at 228.172 kb on + strand, within GFF500 at 228.254 kb on + strand, within GFF500 at 228.254 kb on + strand, within GFF500 at 228.255 kb on - strand, within GFF500 at 228.323 kb on - strand, within GFF500 at 228.423 kb on + strand, within GFF500 at 228.442 kb on + strand, within GFF500 at 228.457 kb on + strand, within GFF500 at 228.828 kb on + strand at 228.829 kb on - strand at 228.889 kb on - strand, within GFF501 at 228.909 kb on - strand, within GFF501 at 228.983 kb on + strand, within GFF501 at 229.157 kb on + strand, within GFF501 at 229.206 kb on + strand, within GFF501 at 229.207 kb on - strand, within GFF501 at 229.207 kb on - strand, within GFF501 at 229.258 kb on + strand, within GFF501 at 229.262 kb on - strand, within GFF501 at 229.322 kb on + strand, within GFF501 at 229.323 kb on - strand, within GFF501
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 225,732 - +0.5 225,732 - +0.3 225,752 + +0.4 225,771 + +1.6 225,820 + GFF499 0.12 -0.5 225,826 + GFF499 0.13 +0.3 225,839 - GFF499 0.15 +0.5 225,839 - GFF499 0.15 -0.4 225,912 + GFF499 0.24 -0.4 226,025 + GFF499 0.39 +1.0 226,025 + GFF499 0.39 +1.0 226,107 - GFF499 0.49 +0.1 226,137 + GFF499 0.53 -0.5 226,137 + GFF499 0.53 +1.1 226,261 - GFF499 0.70 +0.6 226,521 + -0.2 226,524 + +1.3 226,525 - -0.3 226,525 - -0.3 226,610 - -0.7 226,665 + +1.1 227,018 - GFF500 0.20 +0.6 227,051 - GFF500 0.22 -0.3 227,276 + GFF500 0.33 -0.9 227,311 - GFF500 0.34 -0.1 227,475 + GFF500 0.42 -0.1 227,477 + GFF500 0.42 +0.1 227,568 + GFF500 0.47 +0.1 227,632 + GFF500 0.50 -1.2 227,632 + GFF500 0.50 +0.2 227,633 - GFF500 0.50 -0.1 227,686 - GFF500 0.52 +0.5 227,940 + GFF500 0.65 -0.0 227,940 + GFF500 0.65 +0.3 227,946 + GFF500 0.65 -0.1 227,980 + GFF500 0.67 +1.1 227,980 + GFF500 0.67 -0.7 228,039 + GFF500 0.70 +0.1 228,039 + GFF500 0.70 -0.2 228,172 + GFF500 0.76 +0.2 228,254 + GFF500 0.80 +0.2 228,254 + GFF500 0.80 +0.7 228,255 - GFF500 0.80 +0.2 228,323 - GFF500 0.83 +0.4 228,423 + GFF500 0.88 +0.0 228,442 + GFF500 0.89 -0.1 228,457 + GFF500 0.90 -0.3 228,828 + +0.1 228,829 - -0.3 228,889 - GFF501 0.14 +0.2 228,909 - GFF501 0.15 +0.2 228,983 + GFF501 0.21 +0.6 229,157 + GFF501 0.34 -1.2 229,206 + GFF501 0.38 +0.9 229,207 - GFF501 0.38 -1.8 229,207 - GFF501 0.38 +0.7 229,258 + GFF501 0.42 -1.3 229,262 - GFF501 0.42 +1.1 229,322 + GFF501 0.47 -0.9 229,323 - GFF501 0.47 -0.3
Or see this region's nucleotide sequence