Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4843 and GFF4844 are separated by 82 nucleotides GFF4844 and GFF4845 are separated by 149 nucleotides GFF4845 and GFF4846 are separated by 18 nucleotides
GFF4843 - D-glucarate permease, at 143,203 to 144,561
GFF4843
GFF4844 - FIG01046344: hypothetical protein, at 144,644 to 144,892
GFF4844
GFF4845 - Hypothetical flavoprotein YqcA (clustered with tRNA pseudouridine synthase C), at 145,042 to 145,491
GFF4845
GFF4846 - tRNA pseudouridine synthase C (EC 4.2.1.70), at 145,510 to 146,292
GFF4846
Position (kb) 144
145 Strain fitness (log2 ratio) -2
-1
0
1 at 143.772 kb on + strand, within GFF4843 at 143.772 kb on + strand, within GFF4843 at 143.772 kb on + strand, within GFF4843 at 143.778 kb on - strand, within GFF4843 at 143.805 kb on + strand, within GFF4843 at 143.805 kb on + strand, within GFF4843 at 143.806 kb on - strand, within GFF4843 at 143.806 kb on - strand, within GFF4843 at 143.855 kb on - strand, within GFF4843 at 143.883 kb on + strand, within GFF4843 at 143.912 kb on + strand, within GFF4843 at 143.946 kb on + strand, within GFF4843 at 143.947 kb on - strand, within GFF4843 at 143.977 kb on - strand, within GFF4843 at 144.208 kb on - strand, within GFF4843 at 144.237 kb on + strand, within GFF4843 at 144.550 kb on + strand at 144.551 kb on - strand at 144.587 kb on - strand at 144.587 kb on - strand at 144.591 kb on - strand at 144.632 kb on - strand at 144.658 kb on - strand at 144.658 kb on - strand at 144.707 kb on + strand, within GFF4844 at 144.710 kb on - strand, within GFF4844 at 144.765 kb on + strand, within GFF4844 at 144.927 kb on + strand at 144.928 kb on - strand at 144.986 kb on + strand at 145.051 kb on + strand at 145.124 kb on - strand, within GFF4845 at 145.217 kb on - strand, within GFF4845 at 145.260 kb on + strand, within GFF4845 at 145.261 kb on - strand, within GFF4845 at 145.267 kb on - strand, within GFF4845 at 145.344 kb on + strand, within GFF4845 at 145.344 kb on + strand, within GFF4845 at 145.458 kb on - strand at 145.458 kb on - strand at 145.458 kb on - strand at 145.492 kb on + strand at 145.493 kb on - strand at 145.512 kb on - strand at 145.770 kb on + strand, within GFF4846
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 143,772 + GFF4843 0.42 +0.3 143,772 + GFF4843 0.42 +0.2 143,772 + GFF4843 0.42 -0.3 143,778 - GFF4843 0.42 +0.2 143,805 + GFF4843 0.44 +0.4 143,805 + GFF4843 0.44 -0.4 143,806 - GFF4843 0.44 -0.3 143,806 - GFF4843 0.44 +0.2 143,855 - GFF4843 0.48 +0.5 143,883 + GFF4843 0.50 -0.1 143,912 + GFF4843 0.52 -0.1 143,946 + GFF4843 0.55 -0.5 143,947 - GFF4843 0.55 -0.3 143,977 - GFF4843 0.57 -0.2 144,208 - GFF4843 0.74 -0.2 144,237 + GFF4843 0.76 -0.3 144,550 + +1.0 144,551 - +0.9 144,587 - -0.8 144,587 - +0.6 144,591 - +0.6 144,632 - -0.4 144,658 - +0.0 144,658 - -0.3 144,707 + GFF4844 0.25 -0.5 144,710 - GFF4844 0.27 -0.2 144,765 + GFF4844 0.49 -1.5 144,927 + -0.8 144,928 - +0.1 144,986 + -0.4 145,051 + +0.8 145,124 - GFF4845 0.18 -2.5 145,217 - GFF4845 0.39 -0.2 145,260 + GFF4845 0.48 -0.3 145,261 - GFF4845 0.49 -0.1 145,267 - GFF4845 0.50 +1.0 145,344 + GFF4845 0.67 +0.3 145,344 + GFF4845 0.67 +0.0 145,458 - +0.0 145,458 - -0.4 145,458 - -0.6 145,492 + +0.0 145,493 - -2.0 145,512 - +0.1 145,770 + GFF4846 0.33 -0.4
Or see this region's nucleotide sequence