Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4549

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4548 and GFF4549 are separated by 30 nucleotidesGFF4549 and GFF4550 are separated by 3 nucleotidesGFF4550 and GFF4551 are separated by 328 nucleotides GFF4548 - Penicillin-binding protein 2 (PBP-2), at 332,237 to 334,138 GFF4548 GFF4549 - LSU m3Psi1915 methyltransferase RlmH, at 334,169 to 334,636 GFF4549 GFF4550 - Iojap protein, at 334,640 to 334,957 GFF4550 GFF4551 - 'Alpha-ribazole-5'-phosphate phosphatase (EC 3.1.3.73)' transl_table=11, at 335,286 to 335,894 GFF4551 Position (kb) 334 335Strain fitness (log2 ratio) -1 0 1at 334.148 kb on - strandat 334.263 kb on - strand, within GFF4549at 334.386 kb on - strand, within GFF4549at 334.570 kb on - strand, within GFF4549at 334.808 kb on - strand, within GFF4550at 334.814 kb on - strand, within GFF4550at 334.850 kb on - strand, within GFF4550at 334.884 kb on - strand, within GFF4550at 334.884 kb on - strand, within GFF4550at 335.066 kb on - strandat 335.069 kb on - strandat 335.099 kb on - strandat 335.312 kb on + strandat 335.316 kb on + strandat 335.346 kb on + strandat 335.346 kb on + strandat 335.347 kb on - strand, within GFF4551at 335.347 kb on - strand, within GFF4551at 335.347 kb on - strand, within GFF4551at 335.480 kb on + strand, within GFF4551

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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334,148 - +1.4
334,263 - GFF4549 0.20 +1.6
334,386 - GFF4549 0.46 +0.9
334,570 - GFF4549 0.86 +1.2
334,808 - GFF4550 0.53 -0.1
334,814 - GFF4550 0.55 +0.4
334,850 - GFF4550 0.66 +0.2
334,884 - GFF4550 0.77 +0.3
334,884 - GFF4550 0.77 +0.0
335,066 - -0.3
335,069 - -0.3
335,099 - -0.8
335,312 + +0.3
335,316 + -0.4
335,346 + +0.8
335,346 + -0.7
335,347 - GFF4551 0.10 +1.2
335,347 - GFF4551 0.10 -1.0
335,347 - GFF4551 0.10 +0.5
335,480 + GFF4551 0.32 -0.4

Or see this region's nucleotide sequence