Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4400

Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4399 and GFF4400 are separated by 172 nucleotidesGFF4400 and GFF4401 are separated by 116 nucleotidesGFF4401 and GFF4402 are separated by 201 nucleotides GFF4399 - Fosmidomycin resistance protein, at 182,455 to 183,675 GFF4399 GFF4400 - 'UDP-sugar hydrolase (EC 3.6.1.45); 5'-nucleotidase (EC 3.1.3.5)' transl_table=11, at 183,848 to 185,500 GFF4400 GFF4401 - Cys-tRNA(Pro) deacylase YbaK, at 185,617 to 186,096 GFF4401 GFF4402 - FIG00921183: possible ligase, at 186,298 to 187,092 GFF4402 Position (kb) 183 184 185 186Strain fitness (log2 ratio) -2 -1 0 1 2at 182.918 kb on + strand, within GFF4399at 182.951 kb on + strand, within GFF4399at 182.980 kb on + strand, within GFF4399at 183.006 kb on + strand, within GFF4399at 183.329 kb on - strand, within GFF4399at 183.379 kb on - strand, within GFF4399at 183.420 kb on + strand, within GFF4399at 183.421 kb on - strand, within GFF4399at 183.561 kb on + strandat 183.561 kb on + strandat 183.613 kb on - strandat 183.729 kb on + strandat 183.785 kb on + strandat 183.924 kb on - strandat 184.007 kb on + strandat 184.103 kb on - strand, within GFF4400at 184.103 kb on - strand, within GFF4400at 184.193 kb on - strand, within GFF4400at 184.221 kb on + strand, within GFF4400at 184.270 kb on + strand, within GFF4400at 184.271 kb on - strand, within GFF4400at 184.380 kb on + strand, within GFF4400at 184.459 kb on + strand, within GFF4400at 184.652 kb on + strand, within GFF4400at 184.652 kb on + strand, within GFF4400at 184.657 kb on - strand, within GFF4400at 184.724 kb on - strand, within GFF4400at 184.728 kb on - strand, within GFF4400at 184.728 kb on - strand, within GFF4400at 184.728 kb on - strand, within GFF4400at 184.845 kb on + strand, within GFF4400at 184.999 kb on + strand, within GFF4400at 185.098 kb on + strand, within GFF4400at 185.099 kb on - strand, within GFF4400at 185.099 kb on - strand, within GFF4400at 185.101 kb on + strand, within GFF4400at 185.102 kb on - strand, within GFF4400at 185.106 kb on - strand, within GFF4400at 185.107 kb on + strand, within GFF4400at 185.151 kb on - strand, within GFF4400at 185.151 kb on - strand, within GFF4400at 185.183 kb on + strand, within GFF4400at 185.225 kb on + strand, within GFF4400at 185.407 kb on + strandat 185.608 kb on + strandat 185.704 kb on - strand, within GFF4401at 185.718 kb on - strand, within GFF4401at 185.721 kb on + strand, within GFF4401at 185.722 kb on - strand, within GFF4401at 185.964 kb on - strand, within GFF4401at 185.992 kb on - strand, within GFF4401at 185.992 kb on - strand, within GFF4401at 185.994 kb on - strand, within GFF4401at 186.110 kb on - strandat 186.112 kb on + strandat 186.113 kb on - strandat 186.249 kb on - strandat 186.362 kb on + strandat 186.425 kb on - strand, within GFF4402at 186.444 kb on + strand, within GFF4402

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
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182,918 + GFF4399 0.38 -0.5
182,951 + GFF4399 0.41 +0.0
182,980 + GFF4399 0.43 -0.1
183,006 + GFF4399 0.45 +0.5
183,329 - GFF4399 0.72 +0.0
183,379 - GFF4399 0.76 +0.3
183,420 + GFF4399 0.79 -0.5
183,421 - GFF4399 0.79 +0.6
183,561 + +0.2
183,561 + +0.4
183,613 - -0.7
183,729 + +0.5
183,785 + -0.3
183,924 - -0.1
184,007 + -0.4
184,103 - GFF4400 0.15 -0.8
184,103 - GFF4400 0.15 +0.2
184,193 - GFF4400 0.21 -0.5
184,221 + GFF4400 0.23 +0.5
184,270 + GFF4400 0.26 -0.0
184,271 - GFF4400 0.26 +0.6
184,380 + GFF4400 0.32 -0.0
184,459 + GFF4400 0.37 +2.1
184,652 + GFF4400 0.49 +0.5
184,652 + GFF4400 0.49 +0.1
184,657 - GFF4400 0.49 -0.1
184,724 - GFF4400 0.53 +0.1
184,728 - GFF4400 0.53 -1.6
184,728 - GFF4400 0.53 +0.8
184,728 - GFF4400 0.53 +1.4
184,845 + GFF4400 0.60 +0.1
184,999 + GFF4400 0.70 +1.0
185,098 + GFF4400 0.76 -0.0
185,099 - GFF4400 0.76 +1.1
185,099 - GFF4400 0.76 +0.0
185,101 + GFF4400 0.76 -0.4
185,102 - GFF4400 0.76 +0.3
185,106 - GFF4400 0.76 -0.7
185,107 + GFF4400 0.76 -0.2
185,151 - GFF4400 0.79 +0.1
185,151 - GFF4400 0.79 -1.8
185,183 + GFF4400 0.81 +0.2
185,225 + GFF4400 0.83 +1.3
185,407 + -0.2
185,608 + -0.4
185,704 - GFF4401 0.18 +0.2
185,718 - GFF4401 0.21 +0.5
185,721 + GFF4401 0.22 -2.4
185,722 - GFF4401 0.22 -0.5
185,964 - GFF4401 0.72 +0.1
185,992 - GFF4401 0.78 -0.6
185,992 - GFF4401 0.78 +0.2
185,994 - GFF4401 0.79 +0.1
186,110 - -0.7
186,112 + -0.5
186,113 - +1.5
186,249 - -0.7
186,362 + -0.7
186,425 - GFF4402 0.16 -0.7
186,444 + GFF4402 0.18 -0.1

Or see this region's nucleotide sequence