Experiment: LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4313 and GFF4314 are separated by 187 nucleotides GFF4314 and GFF4315 overlap by 26 nucleotides GFF4315 and GFF4316 are separated by 74 nucleotides GFF4316 and GFF4317 are separated by 44 nucleotides GFF4317 and GFF4318 are separated by 3 nucleotides
GFF4313 - Nucleoside-specific channel-forming protein Tsx precursor, at 97,749 to 98,612
GFF4313
GFF4314 - hypothetical protein, at 98,800 to 98,937
GFF4314
GFF4315 - Hypothetical lipoprotein yajI, at 98,912 to 99,409
GFF4315
GFF4316 - hypothetical protein, at 99,484 to 99,630
GFF4316
GFF4317 - Ribonucleotide reductase transcriptional regulator NrdR, at 99,675 to 100,052
GFF4317
GFF4318 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 100,056 to 101,159
GFF4318
Position (kb)
99
100 Strain fitness (log2 ratio)
-1
0
1
2 at 98.617 kb on - strand at 98.617 kb on - strand at 98.617 kb on - strand at 98.617 kb on - strand at 98.675 kb on - strand at 98.697 kb on - strand at 98.739 kb on + strand at 98.740 kb on - strand at 98.802 kb on - strand at 98.833 kb on - strand, within GFF4314 at 98.993 kb on - strand, within GFF4315 at 99.098 kb on + strand, within GFF4315 at 99.150 kb on - strand, within GFF4315 at 99.182 kb on - strand, within GFF4315 at 99.204 kb on - strand, within GFF4315 at 99.482 kb on + strand at 99.569 kb on + strand, within GFF4316 at 99.570 kb on - strand, within GFF4316 at 99.628 kb on + strand at 99.705 kb on - strand at 99.717 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.795 kb on - strand, within GFF4317 at 99.875 kb on + strand, within GFF4317 at 99.875 kb on + strand, within GFF4317 at 99.974 kb on + strand, within GFF4317 at 99.974 kb on + strand, within GFF4317 at 100.050 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with FelixO1_phage (old) 15 MOI remove 98,617 - +0.2 98,617 - +0.4 98,617 - +0.3 98,617 - -0.4 98,675 - -0.5 98,697 - +1.0 98,739 + +0.3 98,740 - -0.4 98,802 - -0.0 98,833 - GFF4314 0.24 +0.1 98,993 - GFF4315 0.16 +0.3 99,098 + GFF4315 0.37 +0.1 99,150 - GFF4315 0.48 -0.4 99,182 - GFF4315 0.54 -0.7 99,204 - GFF4315 0.59 +1.6 99,482 + +1.0 99,569 + GFF4316 0.58 +1.3 99,570 - GFF4316 0.59 +0.2 99,628 + +1.5 99,705 - +1.9 99,717 - GFF4317 0.11 -0.4 99,795 - GFF4317 0.32 +0.2 99,795 - GFF4317 0.32 +1.3 99,795 - GFF4317 0.32 +0.6 99,875 + GFF4317 0.53 +1.3 99,875 + GFF4317 0.53 +0.7 99,974 + GFF4317 0.79 -0.0 99,974 + GFF4317 0.79 +1.6 100,050 + -1.3
Or see this region's nucleotide sequence